A cabacinha (Luffa operculata) é uma espécie que vem sendo utilizada empiricamente na medicina popular contra a sinusite. Seus frutos possuem substâncias farmacêuticas importantes e ainda são desconhecidos estudos relacionados à sua germinação; por isso justifica-se o conhecimento das condições apropriadas que viabilizem a germinação de suas sementes. Avaliou-se a influência de diferentes volumes de água no substrato e temperaturas na germinação e vigor de sementes da cabacinha. Os tratamentos consistiram do umedecimento do substrato com volumes de água equivalentes a 2,0; 2,5; 3,0 e 3,5 vezes o peso do papel seco e as sementes foram mantidas em germinadores regulados nas temperaturas constantes de 25, 30 e 35°C e alternada de 20-30°C. Foram avaliados a porcentagem e velocidade de germinação, comprimento do hipocótilo e das raízes. O delineamento experimental foi inteiramente ao acaso, com os tratamentos distribuídos em esquema fatorial 4x4 (volumes de água x temperaturas), em 4 repetições de 25 sementes. A temperatura constante de 30°C e o volume de água de 2,0 vezes o peso do substrato seco é a melhor combinação para conduzir os testes de germinação em sementes de cabacinha.

Cabacinha (Luffa operculata) is a species that has been used empirically in folk medicine against sinusitis. The fruits have important pharmaceutical substances and studies related to its germination are not available, justifying the determination of the appropriate conditions that make possible the germination of its seeds. Thus, we evaluated the influence of different volumes of water in substrate and temperatures on germination and vigor of cabacinha. The treatments consisted of moistened substrate with water volumes equal to 2.0; 2.5; 3.0 and 3.5 times its weight of the dry paper and the seeds were kept in germination regulated at constant temperatures of 25, 30 and 35°C and 20-30°C alternating. The percentage and speed of germination, hypocotyl and roots of the plants were evaluated. The experimental design was completely randomized with treatments arranged in a 4x4 factorial (volumes of water x temperatures), in 4 replications of 25 seeds. The constant temperature of 30°C and the water volume of 2.0 times the weight of dry substrate is the best combination to lead the germination tests of cabacinha seeds.

A sorologia tem sido o método de escolha para o diagnóstico da toxoplasmose. Devido a isto, padronizamos um ensaio imunoenzimático (ELISA) e comparamos seus resultados com a técnica de imunofluorescência indireta (IFI). A técnica padronizada apresentou na pesquisa de IgG sensibilidade (S) de 96,7% e especificidade (E) de 75%, com valor de predição de positividade (VPP) de 83,3% e de negatividade (VPN) de 94,7%, com uma concordância ajustada (K) de 73,5%. A IFI apresentou S de 83,8%, E de 79,1% com VPP de 83,8 % e VPN de 79,1% com K de 63%. A concordância bruta entre os dois testes (ELISA/IFI) foi de 88,3% para pesquisa de IgG e de 81,5% para pesquisa de IgM, sendo o K de 70,8% para IgG e de 1,3% para IgM, sendo o índice de correlação (r) de 0,556 para IgG e de -0,023 para IgM. Podemos concluir que a ELISA-IgG padronizada é indicada nos processos de triagem sorológica, sendo a ELISA-IgM desaconselhada uma vez que apresentou baixos índices de concordância ajustada com a técnica de referência, sugerindo pouca confiabilidade dos resultados.

Serology has been the most popular method to diagnose toxoplasmosis. Accordingly, this study standardizes an enzyme-linked immunosorbent assay (ELISA) and compares its results with the IFI technique. In the IgG detection test, the standardized technique presented a sensibility (S) of 96.77%, a specificity (SP) of 75%, with a positive predictive value (PPV) of 83.33%, a negative predictive value (NPV) of 94.74%, and an adjusted concordance (K) of 73.50%. The IFI exhibited 83.87% for S, 79.16% for SP, 83.81% for PPV, 79.16% for NPV, and 63% for K. The rough concordance between these two tests (ELISA/IFI) was 88.35% for the IgG detection test and 81.55% for the IgM detection test. K was 70.82% and 1.31% for IgG and IgM, respectively, the correlation index (r) being 0.556 for IgG and -0.023 for IgM. We can conclude that standardized ELISA-IgG is indicated in serologic selection processes, whereas the ELISA-IgM is not recommended for presenting low values for the adjusted concordance with the reference technique, which suggests not very reliable results.

Triatoma brasiliensis is considered as one of the most important Chagas disease vectors in the northeastern Brazil. This species presents chromatic variations which led to descriptions of subspecies, synonymized by Lent and Wygodzinsky (1979). In order to broaden bionomic knowledge of these distinct colour patterns of T. brasiliensis, captures were performed at different sites, where the chromatic patterns were described: Caicó, Rio Grande do Norte (T. brasiliensis brasiliensis Neiva, 1911), it will be called the "brasiliensis population"; Espinosa, Minas Gerais (T. brasiliensis melanica Neiva & Lent 1941), the "melanica population" and Petrolina, Pernambuco (T. brasiliensis macromelasoma, Galvão 1956), the "macromelasoma population". A fourth chromatic pattern was collected in Juazeiro, Bahia the darker one in overall cuticle coloration, the "Juazeiro population". At the sites of Caicó, Petrolina and Juazeiro, specimens were captured in peridomiciliar ecotopes and in wilderness. In Espinosa the specimens were collected only in wilderness, even though several exhaustive captures have been performed in peridomicile at different sites of this municipality. A total of 298 specimens were captured. The average registered infection rate was 15% for "brasiliensis population" and of 6.6% for "melanica population". Specimens of "macromelasoma" and of "Juazeiro populations" did not present natural infection. Concerning trophic resources, evaluated by the precipitin test, feeding eclecticism for the different colour patterns studied was observed, with dominance of goat blood in household surroundings as well as in wilderness