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Resumo A atividade antioxidante do mel de Tetragonisca angustula (TAH) e seu extrato etanólico (TAEE) foram investigados. Os níveis totais de fenólicos (TPC) e flavonóides (TFC) também foram avaliados. Os resultados para TPC foram 19,91 ± 0,38 e 29,37 ± 1,82 mg GAE g-1 e para TFC 0,20 ± 0,02 e 0,14 ± 0,01 mg QE g-1 de TAH e TAEE, respectivamente. As atividades antioxidantes foram 73,29 ± 0,49% e 93,36 ± 0,27% no ensaio DPPH● e 71,73 ± 4,07% e 97,86 ± 0,35% no ABTS●+ para TAH e TAEE, respectivamente. A atividade redutora total foi determinada pelo método de poder redutor (PR) e íon ferrico (Fe III) e os resultados variaram em PR de 151,7 ± 25,7 e 230,7 ± 25,2 mg GAE L-1, para TAH e TAEE respectivamente e para (Fe III) em EC50 0,284 em TAEE e 0,687 em TAH. A análise química por HPLC-DAD do extrato etanólico (TAEE) revelou a presença de ácido ferúlico como componente majoritário no extrato. A análise de RMN 1H confirmou esta estrutura e mostrou a presença de glicose, ácido cítrico, ácido succínico, prolina e derivados de hidrocarbonetos no TAEE. Além disso, a origem botânica também foi investigada e apresentou característica multifloral, tendo encontrado 19 tipos polínicos com predomínio botânico da família Anacardiaceae, sendo o pólen Tapirira predominante (42,6%) e o Schinus secundário (25,7%). Os resultados mostraram que o mel de T. angustula é uma interessante fonte de compostos fenólicos antioxidantes devido à sua origem floral e pode atuar como protetor da saúde humana quando consumido.

Abstract The antioxidant activity of Tetragonisca angustula honey (TAH) and its ethanolic extract (TAEE) were investigated. The total levels of phenolic (TPC) and flavonoids (TFC) were also evaluated. The results for TPC were 19.91 ± 0.38 and 29.37 ± 1.82 mg GAE g-1 and for TFC 0.20 ± 0.02 and 0.14 ± 0.01 mg QE g-1 of TAH and TAEE, respectively. Antioxidant activities were 73.29 ± 0.49% and 93.36 ± 0.27% in the DPPH● assay and 71.73 ± 4.07% and 97.86 ± 0.35% in ABTS●+ for TAH and TAEE, respectively. The total reducing activity was determined by the method of reducing power (PR) and iron ion (Fe III) and the results varied in PR from 151.7 ± 25.7 and 230.7 ± 25.2 mg GAE L-1, for TAH and TAEE respectively and for (Fe III) in EC50 0.284 in TAEE and 0.687 in TAH. Chemical analysis by HPLC-DAD of the ethanolic extract (TAEE) revealed the presence of ferulic acid as majority phenolic component in the extract. The 1H NMR analysis confirmed this structure and showed the also presence of glucose, citric acid, succinic acid, proline and hydrocarbon derivatives. In addition, the botanical origin was also investigated and showed a multifloral characteristic, having found 19 pollen types with a botanical predominance of the Anacardiaceae family, with Tapirira pollen occurring as predominant (42.6%) and Schinus as secondary (25.7%). The results showed that T. angustula honey is an interesting source of antioxidant phenolic compounds due to its floral origin and can act as a protector of human health when consumed.

https://doi.org/10.1590/1519-6984.253599

[SciELO Preprints] - Brazilian Guidelines for In-office and Out-of-office Blood Pressure Measurement – 2023
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Feitosa, Audes Diógenes de Magalhães Barroso, Weimar Kunz Sebba Mion Júnior, Décio Nobre, Fernando Mota-Gomes, Marco Antonio Jardim, Paulo Cesar Brandão Veiga Amodeo, Celso Camargo, Adriana Alessi, Alexandre Sousa, Ana Luiza Lima Brandão, Andréa Araujo Pio-Abreu, Andrea Sposito, Andrei Carvalho Pierin, Angela Maria Geraldo Paiva, Annelise Machado Gomes de Spinelli, Antonio Carlos de Souza Machado, Carlos Alberto Poli-de-Figueiredo, Carlos Eduardo Rodrigues, Cibele Isaac Saad Forjaz, Cláudia Lúcia de Moraes Sampaio, Diogo Pereira Santos Barbosa, Eduardo Costa Duarte Freitas, Elizabete Viana de Cestário , Elizabeth do Espírito Santo Muxfeldt, Elizabeth Silaid Lima Júnior, Emilton Campana, Erika Maria Gonçalves Feitosa, Fabiana Gomes Aragão Magalhães Consolim-Colombo, Fernanda Marciano Almeida, Fernando Antônio de Silva, Giovanio Vieira da Moreno Júnior, Heitor Finimundi, Helius Carlos Guimarães, Isabel Cristina Britto Gemelli, João Roberto Barreto Filho, José Augusto Soares Vilela-Martin, José Fernando Ribeiro, José Marcio Yugar-Toledo, Juan Carlos Magalhães, Lucélia Batista Neves Cunha Drager, Luciano Ferreira Bortolotto, Luiz Aparecido Alves, Marco Antonio de Melo Malachias, Marcus Vinícius Bolívar Neves, Mario Fritsch Toros Santos, Mayara Cedrim Dinamarco, Nelson Moreira Filho, Osni Passarelli Júnior, Oswaldo Valverde de Oliveira Vitorino, Priscila Valverde de Oliveira Miranda, Roberto Dischinger Bezerra, Rodrigo Pedrosa, Rodrigo Pinto Paula, Rogério Baumgratz de Okawa, Rogério Toshiro Passos Póvoa, Rui Manuel dos Santos Fuchs, Sandra C. Inuzuka, Sayuri Ferreira-Filho, Sebastião R. Paffer Fillho, Silvio Hock de Jardim, Thiago de Souza Veiga Guimarães Neto, Vanildo da Silva Koch, Vera Hermina Gusmão, Waléria Dantas Pereira Oigman, Wille Nadruz, Wilson

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Data de publicação: 2023-09-23

Resumo: En Es Pt

Hypertension is one of the primary modifiable risk factors for morbidity and mortality worldwide, being a major risk factor for coronary artery disease, stroke, and kidney failure. Furthermore, it is highly prevalent, affecting more than one-third of the global population. Blood pressure measurement is a MANDATORY procedure in any medical care setting and is carried out by various healthcare professionals. However, it is still commonly performed without the necessary technical care. Since the diagnosis relies on blood pressure measurement, it is clear how important it is to handle the techniques, methods, and equipment used in its execution with care. It should be emphasized that once the diagnosis is made, all short-term, medium-term, and long-term investigations and treatments are based on the results of blood pressure measurement. Therefore, improper techniques and/or equipment can lead to incorrect diagnoses, either underestimating or overestimating values, resulting in inappropriate actions and significant health and economic losses for individuals and nations. Once the correct diagnosis is made, as knowledge of the importance of proper treatment advances, with the adoption of more detailed normal values and careful treatment objectives towards achieving stricter blood pressure goals, the importance of precision in blood pressure measurement is also reinforced. Blood pressure measurement (described below) is usually performed using the traditional method, the so-called casual or office measurement. Over time, alternatives have been added to it, through the use of semi-automatic or automatic devices by the patients themselves, in waiting rooms or outside the office, in their own homes, or in public spaces. A step further was taken with the use of semi-automatic devices equipped with memory that allow sequential measurements outside the office (ABPM; or HBPM) and other automatic devices that allow programmed measurements over longer periods (HBPM). Some aspects of blood pressure measurement can interfere with obtaining reliable results and, consequently, cause harm in decision-making. These include the importance of using average values, the variation in blood pressure during the day, and short-term variability. These aspects have encouraged the performance of a greater number of measurements in various situations, and different guidelines have advocated the use of equipment that promotes these actions. Devices that perform HBPM or ABPM, which, in addition to allowing greater precision, when used together, detect white coat hypertension (WCH), masked hypertension (MH), sleep blood pressure alterations, and resistant hypertension (RHT) (defined in Chapter 2 of this guideline), are gaining more and more importance. Taking these details into account, we must emphasize that information related to diagnosis, classification, and goal setting is still based on office blood pressure measurement, and for this reason, all attention must be given to the proper execution of this procedure.

La hipertensión arterial (HTA) es uno de los principales factores de riesgo modificables para la morbilidad y mortalidad en todo el mundo, siendo uno de los mayores factores de riesgo para la enfermedad de las arterias coronarias, el accidente cerebrovascular (ACV) y la insuficiencia renal. Además, es altamente prevalente y afecta a más de un tercio de la población mundial. La medición de la presión arterial (PA) es un procedimiento OBLIGATORIO en cualquier atención médica o realizado por diferentes profesionales de la salud. Sin embargo, todavía se realiza comúnmente sin los cuidados técnicos necesarios. Dado que el diagnóstico se basa en la medición de la PA, es claro el cuidado que debe haber con las técnicas, los métodos y los equipos utilizados en su realización. Debemos enfatizar que una vez realizado el diagnóstico, todas las investigaciones y tratamientos a corto, mediano y largo plazo se basan en los resultados de la medición de la PA. Por lo tanto, las técnicas y/o equipos inadecuados pueden llevar a diagnósticos incorrectos, subestimando o sobreestimando valores y resultando en conductas inadecuadas y pérdidas significativas para la salud y la economía de las personas y las naciones. Una vez realizado el diagnóstico correcto, a medida que avanza el conocimiento sobre la importancia del tratamiento adecuado, con la adopción de valores de normalidad más detallados y objetivos de tratamiento más cuidadosos hacia metas de PA más estrictas, también se refuerza la importancia de la precisión en la medición de la PA. La medición de la PA (descrita a continuación) generalmente se realiza mediante el método tradicional, la llamada medición casual o de consultorio. Con el tiempo, se han agregado alternativas a través del uso de dispositivos semiautomáticos o automáticos por parte del propio paciente, en salas de espera o fuera del consultorio, en su propia residencia o en espacios públicos. Se dio un paso más con el uso de dispositivos semiautomáticos equipados con memoria que permiten mediciones secuenciales fuera del consultorio (AMPA; o MRPA) y otros automáticos que permiten mediciones programadas durante períodos más largos (MAPA). Algunos aspectos en la medición de la PA pueden interferir en la obtención de resultados confiables y, en consecuencia, causar daños en las decisiones a tomar. Estos incluyen la importancia de usar valores promedio, la variación de la PA durante el día y la variabilidad a corto plazo. Estos aspectos han alentado la realización de un mayor número de mediciones en diversas situaciones, y diferentes pautas han abogado por el uso de equipos que promuevan estas acciones. Los dispositivos que realizan MRPA o MAPA, que además de permitir una mayor precisión, cuando se usan juntos, detectan la hipertensión de bata blanca (HBB), la hipertensión enmascarada (HM), las alteraciones de la PA durante el sueño y la hipertensión resistente (HR) (definida en el Capítulo 2 de esta guía), están ganando cada vez más importancia. Teniendo en cuenta estos detalles, debemos enfatizar que la información relacionada con el diagnóstico, la clasificación y el establecimiento de objetivos todavía se basa en la medición de la presión arterial en el consultorio, y por esta razón, se debe prestar toda la atención a la ejecución adecuada de este procedimiento.

A hipertensão arterial (HA) é um dos principais fatores de risco modificáveis para morbidade e mortalidade em todo o mundo, sendo um dos maiores fatores de risco para doença arterial coronária, acidente vascular cerebral (AVC) e insuficiência renal. Além disso, é altamente prevalente e atinge mais de um terço da população mundial. A medida da PA é procedimento OBRIGATÓRIO em qualquer atendimento médico ou realizado por diferentes profissionais de saúde. Contudo, ainda é comumente realizada sem os cuidados técnicos necessários. Como o diagnóstico se baseia na medida da PA, fica claro o cuidado que deve haver com as técnicas, os métodos e os equipamentos utilizados na sua realização. Deve-se reforçar que, feito o diagnóstico, toda a investigação e os tratamentos de curto, médio e longo prazos são feitos com base nos resultados da medida da PA. Assim, técnicas e/ou equipamentos inadequados podem levar a diagnósticos incorretos, tanto subestimando quanto superestimando valores e levando a condutas inadequadas e grandes prejuízos à saúde e à economia das pessoas e das nações. Uma vez feito o diagnóstico correto, na medida em que avança o conhecimento da importância do tratamento adequado, com a adoção de valores de normalidade mais detalhados e com objetivos de tratamento mais cuidadosos no sentido do alcance de metas de PA mais rigorosas, fica também reforçada a importância da precisão na medida da PA. A medida da PA (descrita a seguir) é habitualmente feita pelo método tradicional, a assim chamada medida casual ou de consultório. Ao longo do tempo, foram agregadas alternativas a ela, mediante o uso de equipamentos semiautomáticos ou automáticos pelo próprio paciente, nas salas de espera ou fora do consultório, em sua própria residência ou em espaços públicos. Um passo adiante foi dado com o uso de equipamentos semiautomáticos providos de memória que permitem medidas sequenciais fora do consultório (AMPA; ou MRPA) e outros automáticos que permitem medidas programadas por períodos mais prolongados (MAPA). Alguns aspectos na medida da PA podem interferir na obtenção de resultados fidedignos e, consequentemente, causar prejuízo nas condutas a serem tomadas. Entre eles, estão: a importância de serem utilizados valores médios, a variação da PA durante o dia e a variabilidade a curto prazo. Esses aspectos têm estimulado a realização de maior número de medidas em diversas situações, e as diferentes diretrizes têm preconizado o uso de equipamentos que favoreçam essas ações. Ganham cada vez mais espaço os equipamentos que realizam MRPA ou MAPA, que, além de permitirem maior precisão, se empregados em conjunto, detectam a HA do avental branco (HAB), HA mascarada (HM), alterações da PA no sono e HA resistente (HAR) (definidos no Capítulo 2 desta diretriz). Resguardados esses detalhes, devemos ressaltar que as informações relacionadas a diagnóstico, classificação e estabelecimento de metas ainda são baseadas na medida da PA de consultório e, por esse motivo, toda a atenção deve ser dada à realização desse procedimento.

https://doi.org/10.1590/SciELOPreprints.7057

Evaluation of pediatric diabetes mellitus after SARS-CoV-2 infection: A long-term prospective case series
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Fink, Thais T.

; Canton, Ana P.M.

; Pereira, Maria Fernanda B.

; Bain, Vera

; Matsuo, Olívia

; Astley, Camilla

; Marques, Heloisa H.S.

; Correa-Silva, Simone

; Montenegro, Marilia M.

; Palmeira, Patricia

; Garanito, Marlene P.

; Duarte, Alberto J.S.

; Carneiro-Sampaio, Magda

; Latronico, Ana C.

; Silva, Clovis A.

Clinics

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2023, Volume 78 elocation 100299

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1016/j.clinsp.2023.100299

Toxicity of Agave sisalana extracts on Cordyceps and their effect and the association with fungi on Nasutitermes corniger (Isoptera: Termitidae)
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Da Silva Lopes, Rosineide

; Barroso Martins, Mônica Cristina

; De Lima, Geiziquele

; Gonçalves De Oliveira, Luciana

; Félix Da Costa, Antonio

; Felipe Dos Santos, Venézio

; Dos Santos Correia, Maria Tereza

; Da Silva, Nicácio Henrique

; Correia De Albuquerque, Auristela

; De Luna Alves Lima, Elza Áurea

; De Menezes Lima, Vera Lúcia

Revista Colombiana de Entomología

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Dez 2022, Volume 48 Nº 2 elocation e11537

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Nasutitermes corniger es una termita arbórea que causa daños económicos en las zonas urbanas, y su control es mediante insecticidas químicos. Una alternativa para el control de insectos es el uso de hongos entomopatógenos y extractos de plantas, o el efecto sinérgico de la asociación de estos. Se examinó la toxicidad de los extractos acuosos e hidroetanólicos de Agave sisalana sobre los aspectos biológicos de los hongos Cordyceps farinosa, C. fumosorosea y C. javanica y la acción de los extractos y su efecto sinérgico sobre N. corniger. Se evaluó el efecto de los extractos sobre hongos por germinación, esporulación y crecimiento micelial de los hongos en medio Sabouraud mediante el estudio de aspectos biológicos bajo condiciones de laboratorio y se examinó el control de N. corniger, por ingestión de extractos y de la asociación extracto y hongo sobre papel de filtro, siendo el porcentaje de mortalidad de soldados y obreras de la termita diaria. En general, los extractos resultaron inofensivos para los hongos a concentraciones de 10, 25, 50, 100 y 200 mg.mL-1. Los extractos demostraron acciones termiticidas a las concentraciones ensayadas, provocando la mortalidad de alrededor del 100% de los trabajadores después del tercer y sexto día (LC50: 0.778 a 0.803 mg.mL-1) y del 100% de los soldados entre el cuarto y séptimo días (LC50: 0.146 a 0.956 mg.mL-1). La asociación de los extractos con C. farinosa ESALQ1355 fue más eficiente para inducir la mortalidad en N. corniger. Estos resultados demostraron la eficiencia in vitro de los extractos de sisal en el control de las termitas de N. corniger, solas o en combinación con hongos, lo que sugiere su potencial utilidad conjunta en el control biológico de plagas de insectos.

Abstract Nasutitermes corniger is an arboreal termite that causes economic damage in urban areas, and its control is for chemical insecticides. An alternative for insect control is the use of entomopathogenic fungi and plant extracts, or the synergistic effect of the association of these. The toxicity of aqueous and hydroethanolic extracts of Agave sisalana on the biological aspects of the fungal Cordyceps farinosa, C. fumosorosea and C. javanica and the action of the extracts and their synergistic effect on N. corniger was examined. The effect of the extracts on fungi was evaluated by germination, sporulation and mycelial growth of fungi in Sabouraud medium through the study of biological aspects under laboratory conditions; and control of N. corniger were examined, by ingestion of extracts and of the association extract plus fungus on filter paper, being the percentage of mortality of soldiers and workers of the termite daily. In general, the extracts were inoffensive to the fungi at concentrations 10, 25, 50, 100 and 200 mg.mL-1. The extracts demonstrated termiticidal actions at concentrations tested, causing the mortalities about 100% of the workers after the third and sixth days (LC50: 0.778 to 0.803 mg.mL-1) and 100% of the soldiers between the fourth and seventh days (LC50:0.146 to 0.956 mg.mL-1). The association of the extracts with C. farinosa ESALQ1355 was more efficient in inducing the mortality in N. corniger. These results demonstrated the efficiency in vitro of the sisal extracts in controlling N. corniger termites, alone or in combination with fungi, suggesting their potential joint usefulness in the biological control of insect pests.

https://doi.org/10.25100/socolen.v48i2.11537

Definition and management of very high fracture risk in women with postmenopausal osteoporosis: a position statement from the Brazilian Society of Endocrinology and Metabolism (SBEM) and the Brazilian Association of Bone Assessment and Metabolism (ABRASSO)
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Silva, Barbara C.

; Madeira, Miguel

; d’Alva, Catarina Brasil

; Maeda, Sergio Setsuo

; Holanda, Narriane Chaves Pereira de

; Ohe, Monique Nakayama

; Szejnfeld, Vera

; Zerbini, Cristiano A. F.

; Paula, Francisco José Albuquerque de

; Bandeira, Francisco

Archives of Endocrinology and Metabolism

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Out 2022, Volume 66 Nº 5 Páginas 591 - 603

Resumo: En | Texto: En | PDF: En

ABSTRACT Several drugs are available for the treatment of osteoporosis in postmenopausal women. Over the last decades, most patients requiring pharmacological intervention were offered antiresorptive drugs as first-line therapy, while anabolic agents were considered a last resource for those with therapeutic failure. However, recent randomized trials in patients with severe osteoporosis have shown that anabolic agents reduce fractures to a greater extent than antiresorptive medications. Additionally, evidence indicates that increases in bone mineral density (BMD) are maximized when patients are treated with anabolic agents first, followed by antiresorptive therapy. This evidence is key, considering that greater increases in BMD during osteoporosis treatment are associated with a more pronounced reduction in fracture risk. Thus, international guidelines have recently proposed an individualized approach to osteoporosis treatment based on fracture risk stratification, in which the stratification risk has been refined to include a category of patients at very high risk of fracture who should be managed with anabolic agents as first-line therapy. In this document, the Brazilian Society of Endocrinology and Metabolism and the Brazilian Association of Bone Assessment and Metabolism propose the definition of very high risk of osteoporotic fracture in postmenopausal women, for whom anabolic agents should be considered as first-line therapy. This document also reviews the factors associated with increased fracture risk, trials comparing anabolic versus antiresorptive agents, efficacy of anabolic agents in patients who are treatment naïve versus those previously treated with antiresorptive agents, and safety of anabolic agents.

https://doi.org/10.20945/2359-3997000000522

Biological properties of Schinus terebinthifolia Raddi essential oil
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Vasconcelos, Priscilla Guimarães Silva

; Alves, Érika Ponchet

; Maia, Carolina Medeiros de Almeida

; Brito, Arella Cristina Muniz

; Silva, Diego Romário

; Freires, Irlan Almeida

; Cavalcanti, Yuri Wanderley

; Rehder, Vera Lucia Garcia

; Ruiz, Ana Lúcia Tasca Gois

; Duarte, Marta Cristina Teixeira

; Rosalen, Pedro Luiz

; Godoy, Gustavo Pina

; Costa, Edja Maria Melo de Brito

Brazilian Journal of Pharmaceutical Sciences

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2022, Volume 58 elocation e20417

Resumo: En | Texto: En | PDF: En

Abstract Schinus terebinthifolia Raddi green fruits essential oil (EO) was evaluated regarding its phytochemical profile, antimicrobial and cytotoxic activities, and toxicity. Gas chromatography with mass spectrometry was applied to identify its constituents, thereafter the minimum inhibitory concentration, minimum bactericidal and fungicidal concentrations, and its antibiofilm activity were evaluated. The EO cytotoxicity was assessed in tumor and non-tumor human cells, and in vivo toxicity was evaluated in a Galleria mellonella model. The major constituents of S. terebinthifolia EO were alpha-phellandrene and beta-phellandrene. The EO had a weak activity against all strains of Candida albicans (MIC 1000μg/mL) and had no activity against non-albicans strains, bacteria, and C. albicans biofilm. Cytostatic activity against all tumor cell lines was shown. Additionally, cell viability remained at EO concentrations up to 62.5 μg/mL. At 16 mg/mL, 50% hemolysis was observed, and it had low toxicity in vivo. Overall, the S. terebinthifolia EO was characterized by low antimicrobial and antibiofilm activities, with no evidence of toxicity to human tumor and non-tumor cells.

https://doi.org/10.1590/s2175-97902021000x2e20417

Identification of genomic imbalances in oral clefts
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Lustosa-Mendes, Elaine

; Santos, Ana P. dos

; Vieira, Társis P.

; Ribeiro, Erlane M.

; Rezende, Adriana A.

; Fett-Conte, Agnes C.

; Cavalcanti, Denise P.

; Félix, Têmis M.

; Monlleó, Isabella L.

; Gil-da-Silva-Lopes, Vera Lúcia

Jornal de Pediatria

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Jun 2021, Volume 97 Nº 3 Páginas 321 - 328

Resumo: En | Texto: En | PDF: En

Abstract Objective This article presents a clinical and cytogenomic approach that focuses on the diagnosis of syndromic oral clefts (OCs). Methods The inclusion criteria were individuals with OC presenting four or more minor signs and no major defects (non-syndromic oral clefts [NSOCs]) as well as individuals with OC presenting at least another major defect, regardless of the number of minor signs (syndromic oral clefts [SOCs]). The exclusion criteria included NSOC with less than four minor signs, SOC with known etiology, as well as atypical oral clefts. Results Of 1647 individuals with OC recorded in the Brazilian Database of Craniofacial Anomalies, 100 individuals were selected for chromosome microarray analysis (CMA). Among these, 44 individuals were clinically classified as NSOC and 56 as SOC. CMA was performed for both groups, and abnormal CMA was identified in 9%, all previously classified as SCO. The clinical and CMA data analyses showed a significant predominance of abnormal CMA in individuals classified as SOC (p = 0.0044); prematurity, weight, length, and head circumference at birth were significantly lower in the group with abnormal CMA. Besides, minor signs were significantly higher in this group (p = 0.0090). Conclusion The rigorous selection of cases indicates that the significant variables could help in early recognition of SOC. This study reinforces the importance of applying the CMA technique to establish the diagnosis of SOC. This is an important and universal issue in clinical practice for intervention, care, and genetic counseling.

https://doi.org/10.1016/j.jped.2020.06.005 65 downloads

Assistance and health care provided to adolescents with chronic and immunosuppressive conditions in a tertiary university hospital during the COVID-19 pandemic
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Lavorato, Sofia S.M.

; Helito, Alberto C.

; Barros, Vera P.M.F.R.

; Roz, Deborah F.P.

; Saccani, Ligia P.

; Martiniano, Lorena V.M.

; Lima, Lívia M.L.

; Lima, Dandara C.C.

; Lourenço, Benito

; Pereira, Rosa M.R.

; Gualano, Bruno

; Silva, Clovis A.

; Queiroz, Ligia B.

Clinics

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2021, Volume 76 elocation e2688

Resumo: En | Texto: En | PDF: En

https://doi.org/10.6061/clinics/2021/e2688 316 downloads

Poor Sleep quality and health-related quality of life impact in adolescents with and without chronic immunosuppressive conditions during COVID-19 quarantine
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Helito, Alberto C.

; Lindoso, Livia

; Sieczkowska, Sofia M.

; Astley, Camilla

; Queiroz, Ligia B.

; Rose, Natalia

; Santos, Claudia Renata P.

; Bolzan, Thalis

; Peralta, Rita María I.A.

; Franco, Ruth R.

; Cominato, Louise

; Pereira, Rosa Maria R.

; Tannuri, Uenis

; Campos, Lucia Maria A.

; Lourenço, Benito

; Toma, Ricardo K.

; Medeiros, Karina

; Watanabe, Andréia

; Grangeiro, Patricia Moreno

; Farhat, Sylvia C.

; Casella, Caio B.

; Polanczyk, Guilherme V.

; Gualano, Bruno

; Silva, Clovis A.

; Sallum, Adriana M. E.

; Iraha, Amanda Y.

; Ihara, Bianca P.

; Mazzolani, Bruna C.

; Martinez, Claudia A.

; Strabelli, Claudia A. A.

; Fonseca, Claudia B.

; Lima, Dandara C. C.

; Setoue, Debora N. D.

; Roz, Deborah F. P.

; Smaira, Fabiana I.

; Roschel, Hamilton

; Miyatani, Helena T.

; Marques, Isabela G.

; Oba, Jane

; Ferreira, Juliana C. O.

; Simon, Juliana R.

; Kozu, Katia

; Saccani, Ligia P.

; Martiniano, Lorena V. M.

; Miranda, Luana C. A.

; Silva, Luiz E. V.

; Laurentino, Moisés F.

; Aikawa, Nadia E.

; Sakita, Neusa K.

; Tanigava, Nicolas Y.

; Pereira, Paulo R. A.

; Palmeira, Patrícia

; Angelo, Simone S.

; Lavorato, Sofia S. M.

; Bernardes, Tamires M.

; Franco, Tathiane C.

; Viana, Vivianne S. L.

; Barros, Vera P. M. F. R.

; Zheng, Yingying

Clinics

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2021, Volume 76 elocation e3501

Resumo: En | Texto: En | PDF: En

OBJECTIVE: To assess the possible factors that influence sleep quality in adolescents with and without chronic immunosuppressive conditions quarantined during the coronavirus disease 2019 (COVID-19) pandemic. METHODS: This cross-sectional study included 305 adolescents with chronic immunocompromised conditions and 82 healthy adolescents. Online surveys were completed, which included questions on socio-demographic data and self-rated healthcare routine during COVID-19 quarantine and the following validated questionnaires: the Pittsburgh Sleep Quality Index (PSQI), Pediatric Quality of Life Inventory 4.0 (PedsQL4.0), and Pediatric Outcome Data Collection Instrument (PODCI). RESULTS: The median current age [14 (10-18) vs. 15 (10-18) years, p=0.847] and frequency of female sex (62% vs. 58%, p=0.571) were similar in adolescents with chronic conditions compared with healthy adolescents. The frequency of poor sleep quality was similar in both groups (38% vs. 48%, p=0.118). Logistic regression analysis, including both healthy adolescents and adolescents with chronic conditions (n=387), demonstrated that self-reported increase in screen time (odds ratio [OR] 3.0; 95% confidence interval [CI] 1.3-6.8; p=0.008) and intrafamilial violence report (OR 2.1; 95% CI 1.2-3.5; p=0.008) were independently associated with poor sleep quality in these adolescents. However, the PODCI global function score was associated with a lower OR for poor sleep quality (OR 0.97; 95% CI 0.94-0.99; p=0.001). Further logistic regression, including only adolescents with chronic conditions (n=305), demonstrated that self-reported increase in screen time (OR 3.1; 95% CI 1.4-6.8; p=0.006) and intrafamilial violence report (OR 2.0; 95% CI 1.2-3.4; p=0.011) remained independently associated with poor quality of sleep, whereas a lower PODCI global function score was associated with a lower OR for sleep quality (OR 0.96; 95% CI 0.94-0.98; p<0.001). CONCLUSION: Self-reported increases in screen time and intrafamilial violence report impacted sleep quality in both healthy adolescents and those with chronic conditions. Decreased health-related quality of life was observed in adolescents with poor sleep quality.

https://doi.org/10.6061/clinics/2021/e3501

10.

Persistent symptoms and decreased health-related quality of life after symptomatic pediatric COVID-19: A prospective study in a Latin American tertiary hospital
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Fink, Thais T.

; Marques, Heloisa H.S.

; Gualano, Bruno

; Lindoso, Livia

; Bain, Vera

; Astley, Camilla

; Martins, Fernanda

; Matheus, Denise

; Matsuo, Olivia M.

; Suguita, Priscila

; Trindade, Vitor

; Paula, Camila S.Y.

; Farhat, Sylvia C.L.

; Palmeira, Patricia

; Leal, Gabriela N.

; Suzuki, Lisa

; Odone Filho, Vicente

; Carneiro-Sampaio, Magda

; Duarte, Alberto José S.

; Antonangelo, Leila

; Batisttella, Linamara R.

; Polanczyk, Guilherme V.

; Pereira, Rosa Maria R.

; Carvalho, Carlos Roberto R.

; Buchpiguel, Carlos A.

; Latronico, Ana Claudia

; Seelaender, Marilia

; Silva, Clovis Artur

; Pereira, Maria Fernanda B.

; Sallum, Adriana M. E.

; Brentani, Alexandra V. M.

; Neto, Álvaro José S.

; Ihara, Amanda

; Santos, Andrea R.

; Canton, Ana Pinheiro M.

; Watanabe, Andreia

; Santos, Angélica C. dos

; Pastorino, Antonio C.

; Franco, Bernadette D. G. M.

; Caruzo, Bruna

; Ceneviva, Carina

; Martins, Carolina C. M. F.

; Prado, Danilo

; Abellan, Deipara M.

; Benatti, Fabiana B.

; Smaria, Fabiana

; Gonçalves, Fernanda T.

; Penteado, Fernando D.

; Castro, Gabriela S. F. de

; Gonçalves, Guilherme S.

; Roschel, Hamilton

; Disi, Ilana R.

; Marques, Isabela G.

; Castro, Inar A.

; Buscatti, Izabel M.

; Faiad, Jaline Z.

; Fiamoncini, Jarlei

; Rodrigues, Joaquim C.

; Carneiro, Jorge D. A.

; Paz, Jose A.

; Ferreira, Juliana C.

; Ferreira, Juliana C. O.

; Silva, Katia R.

; Bastos, Karina L. M.

; Kozu, Katia

; Cristofani, Lilian M.

; Souza, Lucas V. B.

; Campos, Lucia M. A.

; Silva Filho, Luiz Vicente R. F.

; Sapienza, Marcelo T.

; Lima, Marcos S.

; Garanito, Marlene P.

; Santos, Márcia F. A.

; Dorna, Mayra B.

; Aikawa, Nadia E.

; Litvinov, Nadia

; Sakita, Neusa K.

; Gaiolla, Paula V. V.

; Pasqualucci, Paula

; Toma, Ricardo K.

; Correa-Silva, Simone

; Sieczkowska, Sofia M.

; Imamura, Marta

; Forsait, Silvana

; Santos, Vera A.

; Zheng, Yingying

Clinics

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2021, Volume 76 elocation e3511

Resumo: En | Texto: En | PDF: En

OBJECTIVES: To prospectively evaluate demographic, anthropometric and health-related quality of life (HRQoL) in pediatric patients with laboratory-confirmed coronavirus disease 2019 (COVID-19) METHODS: This was a longitudinal observational study of surviving pediatric post-COVID-19 patients (n=53) and pediatric subjects without laboratory-confirmed COVID-19 included as controls (n=52) was performed. RESULTS: The median duration between COVID-19 diagnosis (n=53) and follow-up was 4.4 months (0.8-10.7). Twenty-three of 53 (43%) patients reported at least one persistent symptom at the longitudinal follow-up visit and 12/53 (23%) had long COVID-19, with at least one symptom lasting for >12 weeks. The most frequently reported symptoms at the longitudinal follow-up visit were headache (19%), severe recurrent headache (9%), tiredness (9%), dyspnea (8%), and concentration difficulty (4%). At the longitudinal follow-up visit, the frequencies of anemia (11% versus 0%, p=0.030), lymphopenia (42% versus 18%, p=0.020), C-reactive protein level of >30 mg/L (35% versus 0%, p=0.0001), and D-dimer level of >1000 ng/mL (43% versus 6%, p=0.0004) significantly reduced compared with baseline values. Chest X-ray abnormalities (11% versus 2%, p=0.178) and cardiac alterations on echocardiogram (33% versus 22%, p=0.462) were similar at both visits. Comparison of characteristic data between patients with COVID-19 at the longitudinal follow-up visit and controls showed similar age (p=0.962), proportion of male sex (p=0.907), ethnicity (p=0.566), family minimum monthly wage (p=0.664), body mass index (p=0.601), and pediatric pre-existing chronic conditions (p=1.000). The Pediatric Quality of Live Inventory 4.0 scores, median physical score (69 [0-100] versus 81 [34-100], p=0.012), and school score (60 [15-100] versus 70 [15-95], p=0.028) were significantly lower in pediatric patients with COVID-19 at the longitudinal follow-up visit than in controls. CONCLUSIONS: Pediatric patients with COVID-19 showed a longitudinal impact on HRQoL parameters, particularly in physical/school domains, reinforcing the need for a prospective multidisciplinary approach for these patients. These data highlight the importance of closer monitoring of children and adolescents by the clinical team after COVID-19.

https://doi.org/10.6061/clinics/2021/e3511

11.

Intercalation of Apocarotenoids from Annatto (Bixa orellana L.) into Layered Double Hydroxides
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Perotti, Gustavo F.

; Silva, Felipe F.

; Couto, Ricardo A. A. de

; Lima, Filipe C. D. A.

; Petrilli, Helena M.

; Leroux, Fabrice

; Ferreira, Ana M. C.

; Constantino, Vera R. L.

Journal of the Brazilian Chemical Society

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Nov 2020, Volume 31 Nº 11 Páginas 2211 - 2223

Resumo: En | Texto: En | PDF: En

Synthetic conditions were evaluated to intercalate norbixin into layered double hydroxides (LDH). Norbixin was isolated from a hydrosoluble commercial annatto extract. LDH samples comprising M2+/Al3+ (M = Mg, Zn) were synthesized by coprecipitation and characterized by X-ray diffraction, thermal analysis coupled to mass spectrometry, vibrational spectroscopies, and solid-state 13C nuclear magnetic resonance. Radical scavenging activity of LDH hybrids was monitored by electron paramagnetic resonance towards the stable radical DPPH (1,1-diphenyl-2-picrylhydrazyl). LDH hosts presented more than 50 wt.% of norbixin and d-spacing (003) values of about 2.5 or 3.0 nm depending of the cation LDH composition. Vibrational spectra assignment (supported by density functional theory calculations) confirmed the chemical integrity of norbixin after intercalation into LDH. The inorganic layers were found to enhance the thermal stability of norbixin upon intercalation. The free radical scavenging capacity of norbixin was retained when immobilized into LDH. Considering that zinc and magnesium-LDH matrices are biocompatible and that norbixin presents biological activity, the hybrid materials appear as promising systems for biomedical assessment.

https://doi.org/10.21577/0103-5053.20200065 441 downloads

12.

CHEMICAL COMPOSITION AND ACARICIDAL ACTIVITIES OF Indigofera suffruticosa ESSENTIAL OIL AGAINST TWO-SPOTTED SPIDER MITE
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Silva, Cleideana B. da

; Moraes, Marcilio M. de

; Camara, Claudio A. G. da

; Ribeiro, Nicolle de C.

; Melo, João P. R. de

; Lima, Vera L. M. de

; Navarro, Daniela M. A. F.

Química Nova

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Mar 2019, Volume 42 Nº 3 Páginas 313 - 318

Resumo: En | Texto: En | PDF: En

Laboratory bioassays on fumigation, ovicidal and oviposition deterrence activity of the essential oil from Indigofera suffruticosa and its constituents [eugenol, (E)-asarone, dill apiole, (Z)-asarone, thymol, (E)-nerolidol and safrole] against Tetranychus urticae were carried out. Components of Indigofera leaf oil were characterized by gas chromatography (GC) ad mass spectrometry (GC-MS), which revealed the identification of 17 components, representing 97.7 ± 0.6% of the total oil, with a high percentage of phenylpropanoids (89.9 ± 0.5%), among which eugenol (45.6 ± 0.4%) and (E)-asarone (17.4 ± 0.2%) were major constituents. The ovicidal activity and oviposition deterrence tests revealed that the essential oil as well as its main compounds affected the oviposition and hatchability of mite eggs. Eugenol and thymol were the most potent compounds and were approximately 225-fold more potent than the oil. The acaricidal action exerted by sesquiterpenes and phenylpropanoids of the leaf oil is also discussed. The results suggest that the Indigofera oil and selected components associated with ovicidal activity and oviposition deterrence can serve as the basis for a promising agent that could be used in the formulation of a product for use against the T. urticae.

https://doi.org/10.21577/0100-4042.20170329 720 downloads

13.

Organic fertilization to attenuate water salinity effect on papaya growth
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Sousa, Mônica S. da S.

; Lima, Vera L. A. de

; Brito, Marcos E. B.

; Silva, Luderlândio de A.

; Moreira, Rômulo C. L.

; Oliveira, Carlos J. A.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Fev 2019, Volume 23 Nº 2 Páginas 79 - 83

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO A salinidade da água e do solo reduz o crescimento e a produção de culturas, em especial às fruteiras, como o mamoeiro, sendo necessário obter alternativas de manejo que viabilizem o cultivo sob tais condições. Assim, objetivou-se, com este estudo, avaliar o crescimento e fitomassa do mamoeiro cultivado sob irrigação com águas salinizadas e adubação orgânica. Usou-se o delineamento de blocos casualizados, com os tratamentos distribuídos em esquema fatorial, 5 x 2, constituídos de cinco níveis de salinidade da água de irrigação (0,6; 1,2; 1,8; 2,4 e 3,0 dS m-1) e dois níveis de adubação orgânica (10 e 20 L de esterco bovino por planta), com três repetições, totalizando trinta parcelas experimentais. Foram avaliadas variáveis de crescimento do mamoeiro. As plantas de mamoeiro foram afetadas negativamente pela salinidade da água de irrigação, com maior efeito no número de folhas e na massa seca das folhas, não havendo efeito dos níveis de esterco bovino.

ABSTRACT The salinity of water and soil reduces the growth and production of crops, especially the fruit trees, such as papaya. Thus, it is necessary to obtain management alternatives for cultivation under these conditions. Therefore, the objective of this study was to evaluate the growth and phytomass of papaya cultivated under irrigation with saline water and organic fertilization. An experiment was set up using a randomized block design, with the treatments distributed in a 5 x 2 factorial scheme, consisting of five levels of salinity of irrigation water (0.6, 1.2, 1.8, 2.4 and 3.0 dS m-1) and two levels of organic fertilization (10 and 20 L of bovine manure per plant), with three replications, totaling thirty experimental plots. Growth variables of papaya were evaluated. Papaya plants were negatively affected by irrigation water salinity, with a greater effect on the number of leaves and on dry phytomass of leaves, with no effect of bovine manure levels.

https://doi.org/10.1590/1807-1929/agriambi.v23n2p79-83 699 downloads

14.

Production of chili pepper under organic fertilization and irrigation with treated wastewater
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Silva, Viviane F.

; Bezerra, Carlos V. C.

; Nascimento, Elka C. S.

; Ferreira, Tricia N. F.

; Lima, Vera L. A.

; Andrade, Leando O.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Fev 2019, Volume 23 Nº 2 Páginas 84 - 89

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Com a ocorrência de secas prolongadas na região do Semiárido brasileiro, a escassez hídrica influencia diretamente na agricultura nessa região, de forma que o reuso de água e aplicação de esterco bovino torna-se uma alternativa viável para a produção de pimenta. Nesse contexto, a presente pesquisa foi realizada objetivando-se avaliar a produção sustentável de pimenta malagueta (Capsicum frutescens) sob níveis de irrigação com água residuária tratada e doses de esterco bovino. Foram consideradas as seguintes doses de esterco bovino D1 (0% de esterco e 100% solo), D2 (10% esterco e 90% solo), D3 (20% esterco e 80% solo), D4 (30% esterco e 70% solo), D5 (40% esterco e 60% solo) e D6 (50% esterco e 50% solo), em base de volume. Foram aplicados três níveis de irrigação, baseando-se na necessidade hídrica da cultura (NH), sendo 100% NH (N1), 75% NH (N2) e 50% NH (N3). Foram avaliadas as variáveis de produção e características físicas das pimentas produzidas. Houve aumento da produção de pimenta (g planta-1) ao incrementar 10% de esterco bovino na composição do substrato. O aumento na concentração de esterco bovino e utilizando-se 50% da necessidade hídrica da cultura propiciaram condições favoráveis para a produção de pimenta malagueta orgânica.

ABSTRACT With the occurrence of prolonged droughts in the Brazilian semi-arid region, water scarcity directly influences agriculture in this region, so that water reuse and bovine manure application become a viable alternative for pepper production. In this context, the present study aimed to evaluate the sustainable production of chili peppers (Capsicum frutescens) under levels of irrigation with treated wastewater and doses of bovine manure. The following bovine manure doses were considered: D1 (0% manure and 100% soil), D2 (10% manure and 90% soil), D3 (20% manure and 80% soil), D4 (30% manure and 70% soil), D5 (40% manure and 60% soil) and D6 (50% manure and 50% soil), based on volume. Three levels of irrigation were applied, based on the water requirement of the crop (WR), namely: 100% WR (L1), 75% WR (L2) and 50% WR (L3). Production variables and physical characteristics of the produced peppers were evaluated. Pepper production (g plant-1) increased as 10% of bovine manure were added in substrate composition. Increasing concentration of bovine manure and using 50% WR provided favorable conditions for the organic production of chili pepper.

https://doi.org/10.1590/1807-1929/agriambi.v23n2p84-89 1255 downloads

15.

The use of surface electromyography to assess transfemoral amputees: methodological and functional perspective
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Silva, Rafaella C.

; Zoccoli, Thanyze A. V.

; Marães, Vera R.F.S.

Motriz: Revista de Educação Física

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2019, Volume 25 Nº 3 elocation e101995

Resumo: En | Texto: En | PDF: En

Abstract Aim: Surface electromyography (sEMG) has been established as a safe non-invasive method to investigate neuromuscular function. However, the use of this instrument to assess lower limbs of transfemoral amputees still presents a lack of standardization in its methods of signal acquisition and processing. The aim of this study was to review the current state of sEMG utilization to assess transfemoral amputees, the procedures adopted for the acquisition and the functional findings. Methods: This is a literature review. Five electronic databases were searched to find the studies: All relevant information of each study was extracted and registered. Methodological quality was evaluated using a customized checklist. Results: Eight studies followed the inclusion criteria and were included in this paper. Four studies did not reach more than 80% of the quality checklist, few studies fully described the methodology applied. The muscles assessed were similar in all studies, electrodes placement was determined by different criteria. Conclusion: This paper demonstrates that a few studies have used this method to assess this population and the main variable aspect is concerned to the placement of the electrodes. More researches are needed to better understand the neuromuscular behavior of amputees by using sEMG and assist future researches to develop more reproducible and reliable studies.

https://doi.org/10.1590/s1980-6574201900030012 692 downloads

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