RESUMO A estimativa de fluxo descarga é um componente essencial do planejamento e da tomada de decisões. Está altamente correlacionado com muitas atividades de desenvolvimento envolvendo recursos hídricos. O estudo do transporte de sedimentos nos rios nos ajudará a desenvolver políticas e planos de conservação do solo, controle de enchentes, irrigação, navegação e problemas de biodiversidade aquática. Usando modelos baseados em dados, como Redes Neurais Artificiais (ANNs), a modelagem de vazão e do transporte de sedimentos é frequentemente adotada devido à sua aplicabilidade e capacidade de resolução de problemas. Este estudo utilizou três algoritmos de treinamento como Gradiente Conjugado Escalonado (SCG), Regularização Bayesiana (BR) e Levenberg-Marquardt (LM) para simular o fluxo de vazão e a concentração de sedimentos suspensos (SSC). Depois de otimizar o melhor algoritmo de treinamento baseado nos parâmetros de eficiência do modelo, o modelo LM-ANN foi utilizado para prever o fluxo vazão por dois anos e a modelagem de sedimentos suspensos foi validada com a ajuda de dados observados. O resultado mostrou que os resultados simulados acompanharam o fluxo de vazão, bem como o SSC com a precisão desejada com base nos parâmetros de eficiência do modelo, como coeficiente de determinação (R2), Nash Sutcliffe Efficiency (NSE), Root Mean Square Error (RMSE) e Root Mean Square Deviation (RMSD). Os resultados do estudo revelam que no fluxo de córregos a concentração de sedimentos suspensos é significativamente afetada pelo material de rocha base, geleiras cobertas por detritos e gelo carregado de detritos não consolidados. O transporte dos sedimentos é alto na bacia de Alaknanda em comparação com as outras bacias e os estudos anteriores. Isso pode acontecer devido às severas atividades antropogênicas na bacia circundante.

ABSTRACT The estimation of stream discharge is an essential component of planning and decision-making. It is highly correlated with many development activities involving water resources. The study of transportation of sediments in the rivers will help us to develop policies and plans for soil conservation, flood control, irrigation, navigation, and aquatic biodiversity problems. Using data-driven models such as Artificial Neural Networks (ANNs), modeling of streamflow and sediment transport is frequently adopted due to their applicability and problem-solving ability. This study has used three training algorithms such as Scaled Conjugate Gradient (SCG), Bayesian Regularization (BR), and Levenberg-Marquardt (LM) to simulate the streamflow and Suspended Sediments Concentration (SSC). After optimizing the best training algorithm based on the model efficiency parameters, L-M based-ANN model has been used to predict streamflow for two years and the modeling of suspended sediments was validated with the help of observed data. The result shows that the simulated results tracked the streamflow as well as SSC with the desired accuracy based on the model efficiency parameters such as coefficient of Determination (R2), Nash Sutcliffe Efficiency (NSE), Root Mean Square Error (RMSE), and Root Mean Square Deviation (RMSD). The study's outcomes reveal that in the streamflow the concentration of suspended sediments is significantly affected by the base rock material, glaciers covered by debris, and moraine-laden ice. The transportation of the sediments is high in the Alaknanda basin as compared to the other basins and the previous studies. This might happen due to the severe anthropogenic activities in the surrounding basin.

Owing to better mechanical properties and shape memory effect, the NiTi composites fabricated by powder metallurgy are suitable for biomedical implants. However, the excessive porosity and formation of micro-cracks are the major issues related to the NiTi composite. This work focused on developing crack-free dense NiTi composites by newly developed uni-axial compaction die. The work includes the design and manufacturing of uni-axial compaction die. The die was tested by SOLIDWORKS software in a simulated environment. Further, composite samples were successfully fabricated without circumferential micro-cracks at 1910.82 MPa compaction pressure. The effects of compaction pressure on microstructural, densification, and mechanical behavior of NiTi composites were also analyzed. Microstructural characterization shows that the Ni-rich phase increased and the Ti-rich phase decreased with the increase of compaction pressure. The porosity reduces from 17.04 to 8.75% by increasing the compaction pressure from 1273.88 to 1910.82 MPa, and a maximum density of 5.50 g.cm–3 was obtained. The NiTi150 composite has similar Young’s modulus, and compressive strength (6.93 GPa and 94.36 MPa) compared to cortical and cancellous bone. The high compaction pressure also increases the micro-hardness of NiTi composite up to 453.8 HV0.5.

Resumo Um experimento foi conduzido para estudar a variabilidade genética e o desempenho de nove cultivares exóticas de lilium (Lilium x hybrida) para adequação ao cultivo comercial nas regiões montanhosas do noroeste de Uttarakhand na Estação de Pesquisa e KVK, Lohaghat, GBPUA & T, Pantnagar, Champawat, Uttarakhand, India. Bulbos de nove cultivares híbridos de lilium viz., Acapulco (rosa), Sorbonne (branco), Yelloween (amarelo), Tresor (laranja), Brindisi (rosa), Blackout (vermelho), Pavia (amarelo), Ercolano (branco) e Samur (rosa) foram selecionados como material experimental. Houve diferença significativa nos dias de iniciação das gemas entre as nove cultivares. É evidente, a partir dos dados que demoram dias mínimos para o início da brotação na cultivar Blackout (44 dias), seguido de Tresor (45 dias). O número máximo de flores por planta relatado nas cultivares Acapulco (10,20) seguido por Yelloween (8,20) e Sorbonne (7,80) que está no mesmo nível de Blackout (7,40) e Pavia (7,20). A vida máxima de vaso foi registrada com Acapulco (12,4 dias) seguido por Sorbonne (11,80 dias), que está em paridade com Yelloween (11,8 dias), enquanto a vida mínima de vaso foi registrada com Ercolano (8,40 dias) e Tresor (8,40 dias). O valor médio encontrado foi máximo para o comprimento do caule da flor (87,29) e o valor médio mínimo foi exibido para o número de flores por planta. O valor de herdabilidade foi encontrado máximo para caracteres como dias para a abertura do botão da flor seguido pelo comprimento do caule da flor e dias necessários para o início da brotação.

Abstract An experiment was conducted to study genetic variability and performance of nine exotic lilium cultivars (Lilium x hybrida) for suitability for commercial cultivation in North Western hilly regions of Uttarakhand at Research Station and KVK, Lohaghat, GBPUA&T, Pantnagar, Champawat, Uttarakhand India. Bulbs of nine lilium hybrid cultivars viz., Acapulco (Pink), Sorbonne (White), Yelloween (Yellow), Tresor (Orange), Brindisi (Pink), Blackout (Red), Pavia (Yellow), Ercolano (White) and Samur (Pink) were selected as experimental material. There was significant difference in days taken for bud initiation among nine cultivars. It is evident from data that minimum days take for bud initiation in cultivar Blackout (44 days) followed by Tresor (45 days). The maximum number of flowers per plant reported in cultivars Acapulco (10.20) followed by Yelloween (8.20) and Sorbonne (7.80) which is at par with Blackout (7.40) and Pavia (7.20). Maximum vase life was recorded with Acapulco (12.4 days) followed by Sorbonne (11.80 days) which is at par with Yelloween (11.8 days) while Minimum vase life was recorded with Ercolano (8.40 days) and Tresor ( 8.40 days). Mean value was found maximum for flower stem length (87.29) and minimum mean value was exhibited by number of flowers per plant. Heritability value was found maximum for characters like days to flower bud opening followed by length of flower stalk and days taken to bud initiation.

Abstract: With standardized screening tools, research studies have shown that developmental disabilities can be detected reliably and with validity in children as young as 4 months of age by using the instruments such as the Ages and Stages Questionnaire. In this review, we will focus on one tool, the Ages and Stages Questionnaire, to illustrate the usefulness of developmental screening across the globe.

Resumen: Mediante el uso de herramientas de evaluación estandarizada, algunos estudios de investigación han demostrado que discapacidades de desarrollo se pueden detectar con fiabilidad y validez en niños desde los 4 meses de edad mediante el uso de los instrumentos estandarizados como el Ages and Stages Questionnaire (Cuestionario de las Edades y Etapas). Para ilustrar la utilidad de la evaluación del desarrollo infantil a escala global, en este trabajo se revisará la herramienta Ages and Stages Questionnaire.

Organizations of today face numerous challenges, and training their sales force is definitely high on its agenda. This paper endeavors to contribute to the sales training field by providing a comprehensive stateof- the art and contemporary review through the study of publications in sales training research in the last 30 years (1985-2014). The specific objectives of the review are to study the chronological trends and structural distribution of sales training research in terms of the research type, research focus, methods, research themes and other issues such as methodology, analytical techniques, geographical region etc. Findings of 56 articles on sales training research published in the last three decades are analyzed and directions for future research are identified.

Las organizaciones actuales se enfrentan a numerosos retos, siendo prioritaria en su agenda la formación de su fuerza de ventas. El presente documento pretende contribuir en este campo, mediante la aportación de una revisión amplia, vanguardista y contemporánea del estudio de las publicaciones sobre la investigación de la formación en ventas durante los últimos 30 años (1985-2014). Los objetivos específicos de la revisión son el estudio de las tendencias cronológicas y la distribución estructural de la investigación de la formación en ventas, en términos de tipo, objetivo y temas de la investigación, así como otras cuestiones tales como la metodología, las técnicas analíticas, la región geográfica, etc. Se analizan los hallazgos de 56 artículos sobre investigación de la formación en ventas, publicados durante los últimos treinta años, y se identifican las orientaciones de la investigación futura.

O efeito de doses sub-letais (40% e 80% de LC50/24h) de moluscicidas derivados de plantas com combinações unitárias, binárias (1:1) e terciárias (1:1:1) de Rutin, ácido Elágico, Betulin e taraxerol com látex da J. gossypifolia, folhas e extrato em pó de casca de caule e seus componentes ativos foram estudados na reprodução do caramujo de água fresca Lymnaea acuminata. Foi observado que o látex da J. gossypifolia, casca do caule, folhas individualmente e suas combinações com componentes moluscicidas ativos derivados de outras plantas causaram redução significante na fecundidade, incubação e sobrevivência dos caramujos jovens. Acredita-se que a exposição sub-letal destes moluscicidas sobre a reprodução dos caramujos é processo complexo envolvendo mais de um fator na redução da capacidade reprodutiva.

The effect of sub-lethal doses (40% and 80% of LC50/24h) of plant derived molluscicides of singly, binary (1:1) and tertiary (1:1:1) combinations of the Rutin, Ellagic acid, Betulin and taraxerol with J. gossypifolia latex, leaf and stem bark powder extracts and their active component on the reproduction of freshwater snail Lymnaea acuminata have been studied. It was observed that the J. gossypifolia latex, stem bark, individual leaf and their combinations with other plant derived active molluscicidal components caused a significant reduction in fecundity, hatchability and survival of young snails. It is believed that sub-lethal exposure of these molluscicides on snail reproduction is a complex process involving more than one factor in reducing the reproductive capacity.

The present study was conducted on the 24 healthy and normal embryos/foeti of non- descript goat (Capra hircus) varying from day old to 150 days of gestation. The embryo/foeti were assigned into three groups according to their gestational ages; Groups I (0-50 days), II (51-100 days) and III (101-till term). At 38 days of gestation the primordia of right and left adrenal gland were observed as a pea shaped spherical swelling located just cranial to the respective metanephros. Biometrical studies revealed the increase in the all parameters of the adrenal gland with the advancement of age. Adrenal glands were harvested first grossly at 50 days of gestation.

El presente estudio se realizó en 24 embriones / fetos de cabra (Capra hircus) sanos de un día hasta los 150 días de gestación. Los embriónes fueron separados en tres grupos de acuerdo a la edad gestacional: grupos I (0-50 días), II (51 a 100 días) y III (101-till plazo). A los 38 días de gestación se observó el primordio de las glándulas suprarrenales derecha e izquierda como una protuberancia de forma esférica, situada justo en la zona craneal en relación al metanefros. C on el avance de la edad, estudios biométricos revelan aumento de todos los parámetros de la glándula suprarrenal. Las glándulas suprarrenales fueron extraídas inicialmente a los 50 días de gestación.

Las hojas y semillas de alholva o fenogreco (Trigonella foenum-graecum L.) se han utilizado ampliamente con fines medicinales. Se conoce que las semillas de alholva presentan propiedades antidiabéticas y efectos tales como hipocolesterolémico, anti-cancerígeno y tiroxina inducida de la hiperglucemia. Los resultados de los experimentos realizados revelan una considerable variabilidad entre los genotipos de alholva. Se diferencian en la morfología, hábito de crecimiento, biomasa, y capacidad de producción de semillas. Los componentes químicos de la semilla como por ejemplo los contenidos de polifenol, ácido fítico, saponina, carbohidratos, proteínas y análisis proximal (% de humedad, cenizas, fibra, Zn, Fe, Mn, y Mg) también diferían notablemente. Esta variabilidad es a menudo pasado por alto o es subestimada en los ensayos clínicos. Los resultados sugieren que la variabilidad genética y la interacción genotipo x ambiente desempeñarán un papel importante cuando el cultivo es utilizado por la industria nutracéutica. Además la variabilidad de rasgos importantes de la alholva indican que su base genética, permite la selección para el mejoramiento de los niveles de estos rasgos. La alholva juega un papel importante para avanzar hacia la explotación de su cultivo y superar la deficiencia de micronutrientes en los seres humanos. Los temas asociados para fortalecer la biofortificación de cultivos a través de la investigación y desarrollo de la alholva, serán tratados en esta publicación.

Fenugreek (Trigonella foenum-graecum L.) leaves and seeds have been used extensively for medicinal purposes. Fenugreek seed is known to exhibit anti-diabetic properties and effects such as hypocholesterolaemic, anticancerous and thyroxine-induced hyperglycaemia. The result of the present experiment reveals considerable variability among fenugreek genotypes. They differ in morphology, growth habit, biomass and seed production capability. Chemical constituents of the seed, e.g. polyphenol, phytic acid, saponin, carbohydrate, protein and proximate analysis (moisture %, ash, fiber, Zn, Fe, Mn, and Mg) contents also differed markedly. This variability is most often overlooked or underestimated in clinical trials. Our research suggests that the genetic variability and the genotype by environmental interaction will play a significant role when the crop is used by the nutraceutical industry. Our research results indicate that the variability for important traits in fenugreek have a genetic base, making selection for improved levels of these traits possible. Fenugreek plays a major role to progress towards the exploitation of crop to overcome micronutrient deficiency in human beings. The associated issues to strengthen crop biofortification through fenugreek research and development have been dealt in this piece of research work.

En la India, los recursos acuáticos de agua dulce están sufriendo debido a la creciente población humana, la urbanización y la escasez de todo tipo de recursos naturales como el agua. Para mitigar esto, se ha planificado bajo un amplio esquema que todos los grandes ríos estén interconectados a través de un sistema de canales, sin embargo, la información básica sobre las condiciones ecológicas de los ríos tropicales y sus comunidades de peces es escasa en la actualidad. En vista de ello, el presente estudio se realizó para evaluar el estado ecológico, mediante la comparación de los parámetros tróficos de la comunidad de peces, estado de conservación y el agua de los dos ríos tropicales de la cuenca del Ganges, de octubre 2007 a noviembre 2009. El análisis de los nichos tróficos de las especies de peces disponibles indican dominancia de carnívoros (19 especies) en el río Ken y omnívoros (23 especies) en Betwa. El nivel trófico de las especies carnívoras fue similar en ambos ríos (33.33%), mientras que las especies omnívoras fueron mayores en Betwa (36.51%) que en Ken (28.07%). Sitios relativamente inalterados de Betwa (B1, B2 y B3) y Ken (K2, K3 y K5) se caracterizaron por la alta diversidad y riqueza de especies de peces amenazadas. Los puntajes medios más altos del nivel trófico se registraron en B4 de Betwa y K4 de Ken. El índice de Bray-Curtis para el nivel trófico identificó las especies carnívoras (>0.32) como indicadoras de contaminación. Exposición antropogénica, reflejada en la calidad del agua, así como en la estructura de la comunidad de peces, fue más alta, especialmente en los tramos inferiores de ambos ríos. Nuestros resultados sugieren la importancia de métricas tróficas en la comunidad de peces, para la evaluación de las condiciones ecológicas, lo que permite hacer predicciones sobre el efecto de futuros cambios morfodinámicos (en las fases de post-interconexión), y proporcionar un marco y condición de referencia para apoyar los esfuerzos de restauración de hábitat de peces relativamente alterados en los ríos tropicales de la India.

In India, freshwater aquatic resources are suffering from increasing human population, urbanization and shortage of all kind of natural resources like water. To mitigate this, all the major rivers have been planned for a river-interlinking through an interlinking canal system under a huge scheme; yet, the baseline information on ecological conditions of those tropical rivers and their fish communities is lacking at present. In view of that, the present study was undertaken to assess the ecological condition by comparing the trophic metrics of the fish community, conservation status and water chemistry of the two tropical rivers of the Ganga basin, from October 2007 to November 2009. The analysis of trophic niches of the available fish species indicated dominancy of carnivorous (19 species) in river Ken and omnivorous (23 species) in Betwa. The trophic level score of carnivorous species was recorded similar (33.33%) in both rivers, whereas omnivorous species were mostly found in Betwa (36.51%) than Ken (28.07%). Relatively undisturbed sites of Betwa (B1, B2 and B3) and Ken (K2, K3 and K5) were characterized by diverse fish fauna and high richness of threatened species. The higher mean trophic level scores were recorded at B4 of Betwa and K4 of Ken. The Bray-Curtis index for trophic level identified the carnivorous species (>0.32) as an indicator species for pollution. Anthropogenic exposure, reflected in water quality as well as in fish community structure, was found higher especially in the lower stretches of both rivers. Our results suggest the importance of trophic metrics on fish community, for ecological conditions evaluation, which enables predictions on the effect of future morphodynamic changes (in the post-interlinking phases), and provide a framework and reference condition to support restoration efforts of relatively altered fish habitats in tropical rivers of India.

Pollution control measures have resulted in replacement of chlorine by peroxide as bleaching chemical. Change of chemical affects corrosion aspects, the suitability of existing plant metallurgy and materials of construction of bleach plants. Accordingly long term immersion and electrochemical corrosion tests were conducted on stainless steel 304L, 316L, 2205 and 6% Mo and mild steel in peroxide solutions of pH 10. The materials were tested for uniform corrosion, pitting and crevice corrosion and attack around the weld area. Corrosion attack estimated from long term immersion tests is found in agreement, by and large, with that analyzed from electrochemical test. E-pH diagrams drawn for water-peroxide system have been used to understand the corrosivity of the peroxide media. An attempt has been made to suggest a suitable material of construction for handling the test media on the basis of degree of corrosion attack on them and their cost and the mechanical properties.

O presente trabalho foi levado a efeito para avaliar a atividade moluscicida do ingrediente ativo Taraxerol junto com o extrato de acetona da Euphorbia tirucalli contra o caramujo Lymnaea acuminata. Foram estudados a fecundidade, o desenvolvimento e sobrevivência do caramujo Lymnaea acuminata exposto a estes extratos. Os efeitos danosos destes extratos testados na vida do caramujo L. acuminata foram também avaliados e este estudo demonstra os efeitos inibidores destes extratos isolados ou em combinação binária (relação l:1). Concluiu-se que estes produtos vegetais agem como fontes potenciais de moluscicidas e que eles também têm a vantagem de obtenção fácil, baixo custo, biodegradabilidade e maior aceitação entre os usuários do que os pesticidas sintéticos.

The present work was carried out to evaluate the molluscicidal activity of active ingredient Taraxerol with the acetone extract of Euphorbia tirucalli against Lymnaea acuminata snail. The (fecundity, hatchability and survivability) of snail L. acuminata exposed to this extract was studied. The effects of the tested extracts on life-history traits of harmful snail L. acuminata have also been evaluated, and this study also expounds the inhibitory effects of these extracts singly as well as in binary combination (1:1 ratio). It concluded that these herbal products act as a potential source of molluscicides, and that they would also have the advantage of easy availability, low cost, biodegradability and greater acceptance amongst users than synthetic pesticide.

Channa punctatus foi exposta a quatro diferentes concentrações de Rutina, Taraxerol e Apigenina. Alterações de alguns parâmetros hematológicos da Channa punctatus foram acessados para determinar a influência destes compostos no peixe teste. Peixes foram expostos a concentrações sub-letais (80% 0f LC50 em 24h) destes compostos por uma semana. Os peixes controles foram também expostos durante uma semana. A seguir, amostras de sangue foram obtidas do peixe controle e do experimental. O sangue foi estudado por parâmetros hematológicos selecionados (hematócrito, hemoglobina, contagem de células vermelhas e brancas, proteína plasmática total e concentração de glucose plasmática). Os índices hematológicos derivados da média da concentração corpuscular da hemoglobina (MCHC), a média de hemoglobina corpuscular (MCH) e a média de volume corpuscular (MCV), foram calculados. Concentrações sub-letais destes compostos causaram decréscimo dose-dependente dos valores da hemoglobina unidos a decréscimo de valores de hematócrito e das contagens de células sanguíneas vermelhas o que caracteriza indicação óbvia de anemia. As contagens totais de células brancas e a contagem diferencial destas células estavam diminuídas exceto pelos linfócitos que mostraram leve aumento. A proteína plasmática e a glicose estavam também baixas nos peixes expostos quando comparados com o controle. Os índices hematológicos MCH, MCHC, MCV estavam também diminuídos. Os resultados deste estudo revelam alto percentual de mortalidade e conseqüências deletérias à saúde de peixes submetidos à exposição aguda de Rutina, Talaxerol e Apigenina e portanto eles não devem ser usados diretamente em aquacultura sem conhecimento apropriado.

Channa punctatus was exposed to four different concentrations of Rutin, Taraxerol and Apigenin. Changes in some hematological parameters of Channa punctatus were assessed to determine the influence of these compounds on test fish. Fish were exposed to sublethal concentrations (80% of LC50 of 24h) of these compounds for one week. Control fish were also administered for one week. Thereafter, blood samples were obtained from the control and experimental fish. Blood was assayed for selected hematological parameters (hematocrit, hemoglobin, red blood cell count, white blood cell count total plasma protein and plasma glucose concentration). The derived hematological indices of mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH) and mean corpuscular volume (MCV) were calculated. Sublethal concentrations of these compounds caused a dose dependent decrease in hemoglobin values coupled with a decrease in hematocrit values and red blood cell counts are an obvious indication of anemia. The total white blood cell counts and the differential white blood cell counts were decreased except for the lymphocytes, where there was a slight increase. Plasma protein and glucose were also lower in exposed fish when compared with control. The hematological indices MCH, MCHC, MCV were also lowered. The result from this study reveals high mortality rate and deleterious consequences on the health of fish subjected to acute exposure of Rutin, Taraxerol and Apigenin and therefore, should not be used directly in aquaculture without having the proper knowledge.

Os efeitos tóxicos das combinações binárias e terciárias do pó de látex da Euphorbia hirta Linn assim como outros compostos vegetais moluscicidas foram avaliados em sua ação sobre caramujos de água doce Lymnaea (Radix) acuminata e Indoplanorbis exustus em represas. Estas combinações mostraram significante efeito dose e tempo dependente contra ambos os caramujos. Estes compostos em doses altas foram também letais para peixes de água doce Channa punctatus (Bloch) (Channidae {Ophicephalidae}), que compartilham o ambiente com estes caramujos mas a dose LC90 (24h) para caramujos aparentemente não tem propriedade de matar as populações de peixes quando uma população mista de peixes e caramujos são tratadas.

The toxic effect of binary and tertiary combinations of Euphorbia hirta Linn latex powder with other plant molluscicidal compounds, were evaluated against the freshwater snails Lymnaea (Radix) acuminata and Indoplanorbis exustus in pond. These combinations showed significant time and dose dependent effect against both the snails. These compounds at higher doses were also lethal to freshwater fish Channa punctatus (Bloch) (Channidae {Ophicephalidae}), which shares the habitat with these snails, but the LC90 (24h) doses of snails have no apparent killing properties in fish populations when treated in mixed population of snails and fish.

Staphylococcal food poisoning (SFP) is caused by the members of superantigen family called staphylococcal enterotoxins (SEs). About 20 different types of SEs are produced by Staphylococcus aureus out of which type A (SEA), B (SEB), C (SEC) and D (SED) are commonly implicated in SFP. Among these, SEB is the most potent toxin and has also gained the status of biological warfare (BW) agent. Therefore, detection of SEB is of utmost importance. Any immunological detection system for SEB requires specific and sensitive antibodies which inturn depends on the purity of the SEB. In the present investigation, seb gene of S. aureus was cloned and expressed in E. coli along with biotin as fusion partner to facilitate the purification process. The yield of purified recombinant SEB was 13.1 mg/L of culture broth. Biotin tag from the biotinylated toxin was removed by protease cleavage, and both biotinylated and non-biotinylated toxin types were used for raising hyperimmune antiserum. Antisera were also specific for SEB amongst different kinds of food poisoning agents tested by indirect plate ELISA and western blot analysis. The quality of the antisera raised in this study was found superior to the commercially available antiserum. The investigation suggests that construction of recombinant staphylococcal enterotoxin B is a good alternative for production of pure enterotoxin to be used in antibody generation.