Abstract Filamentous fungi are the organisms of choice for most industrial biotechnology. Some species can produce a variety of secondary metabolites and enzymes of commercial interest, and the production of valuable molecules has been enhanced through different molecular tools. Methods for genetic manipulation and transformation have been essential for the optimization of these organisms. The genus Simplicillium has attracted increased attention given several potential biotechnological applications. The Simplicillium genus harbors several entomopathogenic species and some isolates have been explored for bioremediation of heavy metal contaminants. Furthermore, the myriad of secondary metabolites isolated from Simplicillium spp. render these organisms as ideal targets for deep exploration and further biotechnological mining possibilities. However, the lack of molecular tools hampered the exploration of this genus. Thus, an Agrobacterium tumefaciens-mediated transformation method was established for Simplicillium subtropicum, employing the far-red fluorescent protein TURBOFP635/Katushka, as a visual marker, and the selection marker SUR gene, that confers resistance to chlorimuron ethyl. Notably, one round of transformation using the established method yielded almost 400 chlorimuron resistant isolates. Furthermore, these transformants displayed mitotic stability for, at least, five generations. We anticipate that this method can be useful for deep molecular exploration and improvement of strains in the Simplicillium genus.

RESUMO OBJETIVO Apresentar opções estratégicas para apoiar a adoção de políticas de fortalecimento da saúde mental de universitários da área da saúde, a serem implementadas por instituições universitárias. MÉTODOS Revisão rápida, sem delimitação de período, com buscas realizadas de maio a junho de 2020, em 21 fontes de dados bibliográficos, incluindo literatura cinzenta. Utilizaram-se as palavras-chave: saúde mental, estudantes e universidade. O processo de seleção priorizou revisões sistemáticas sobre intervenções em saúde mental para estudantes universitários em cursos da área da saúde, e considerou, também, outros tipos de revisão e estudos primários relevantes. RESULTADOS Foram incluídos 45 estudos: 34 revisões sistemáticas, uma síntese de evidências, um overview, uma revisão de escopo, três revisões narrativas, três relatos de experiência e dois artigos de opinião. As evidências desses estudos apoiaram a elaboração de quatro opções: 1) estabelecer e apoiar políticas de fortalecimento da saúde mental de estudantes dos cursos da área da saúde; 2) integrar programas de atenção à saúde mental, ampliar sua oferta e facilitar seu acesso pelos estudantes; 3) promover programas educacionais e estratégias de comunicação relacionadas ao sofrimento psíquico contemporâneo e ao seu enfrentamento, para que os estudantes conheçam os serviços e recursos e identifiquem práticas de fortalecimento; 4) monitorar e avaliar continuamente as necessidades em saúde mental dos estudantes dos cursos da área da saúde. CONCLUSÕES As opções são desafiadoras e exigem que as universidades estabeleçam comissões institucionais para implementar uma política de fortalecimento da saúde mental dos estudantes universitários da área da saúde, com capacidade de reconhecer as diversas necessidades em saúde, incluindo as manifestações de sofrimento psíquico; integrar ações internas da universidade entre si e aos serviços do Sistema Único de Saúde; implementar e monitorar as ações que compõem a política de saúde mental.

ABSTRACT OBJECTIVE To present strategic options to support the adoption of mental health strengthening policies for university students in the field of health, to be implemented by university institutions. METHODS Rapid review, without period delimitation, with searches carried out from May to June 2020, in 21 sources of bibliographic data, including gray literature. The following keywords were used: mental health, students and university. The selection process prioritized systematic reviews of mental health interventions for university students in health care courses, and also considered other types of review and relevant primary studies. RESULTS Forty-five studies were included: 34 systematic reviews, an evidence synthesis, an overview, a scope review, three narrative reviews, three experience reports and two opinion articles. The evidence from these studies supported the development of four options: 1) to establish and support policies to strengthen the mental health of students in health care courses; 2) to integrate mental health care programs, expand their offer and facilitate access by students; 3) to promote educational programs and communication strategies related to contemporary psychic suffering and its confrontation, so that students can get to know the services and resources and identify strengthening practices; 4) to continuously monitor and assess the mental health needs of students in health care courses. CONCLUSIONS The options are challenging and require universities to establish institutional commissions to implement a policy to strengthen the mental health of university students in the health area, with the ability to recognize the different health needs, including manifestations of psychic suffering ; to integrate the university’s internal actions with each other and with the services of the Unified Health System; to implement and monitor the actions that make up the mental health policy.

RESUMO OBJETIVO Apresentar opções estratégicas para apoiar a adoção de políticas de fortalecimento da saúde mental de universitários da área da saúde, a serem implementadas por instituições universitárias. MÉTODOS Revisão rápida, sem delimitação de período, com buscas realizadas de maio a junho de 2020, em 21 fontes de dados bibliográficos, incluindo literatura cinzenta. Utilizaram-se as palavras-chave: saúde mental, estudantes e universidade. O processo de seleção priorizou revisões sistemáticas sobre intervenções em saúde mental para estudantes universitários em cursos da área da saúde, e considerou, também, outros tipos de revisão e estudos primários relevantes. RESULTADOS Foram incluídos 45 estudos: 34 revisões sistemáticas, uma síntese de evidências, um overview, uma revisão de escopo, três revisões narrativas, três relatos de experiência e dois artigos de opinião. As evidências desses estudos apoiaram a elaboração de quatro opções: 1) estabelecer e apoiar políticas de fortalecimento da saúde mental de estudantes dos cursos da área da saúde; 2) integrar programas de atenção à saúde mental, ampliar sua oferta e facilitar seu acesso pelos estudantes; 3) promover programas educacionais e estratégias de comunicação relacionadas ao sofrimento psíquico contemporâneo e ao seu enfrentamento, para que os estudantes conheçam os serviços e recursos e identifiquem práticas de fortalecimento; 4) monitorar e avaliar continuamente as necessidades em saúde mental dos estudantes dos cursos da área da saúde. CONCLUSÕES As opções são desafiadoras e exigem que as universidades estabeleçam comissões institucionais para implementar uma política de fortalecimento da saúde mental dos estudantes universitários da área da saúde, com capacidade de reconhecer as diversas necessidades em saúde, incluindo as manifestações de sofrimento psíquico; integrar ações internas da universidade entre si e aos serviços do Sistema Único de Saúde; implementar e monitorar as ações que compõem a política de saúde mental.

ABSTRACT OBJECTIVE To present strategic options to support the adoption of mental health strengthening policies for university students in the field of health, to be implemented by university institutions. METHODS Rapid review, without period delimitation, with searches carried out from May to June 2020, in 21 sources of bibliographic data, including gray literature. The following keywords were used: mental health, students and university. The selection process prioritized systematic reviews of mental health interventions for university students in health care courses, and also considered other types of review and relevant primary studies. RESULTS Forty-five studies were included: 34 systematic reviews, an evidence synthesis, an overview, a scope review, three narrative reviews, three experience reports and two opinion articles. The evidence from these studies supported the development of four options: 1) to establish and support policies to strengthen the mental health of students in health care courses; 2) to integrate mental health care programs, expand their offer and facilitate access by students; 3) to promote educational programs and communication strategies related to contemporary psychic suffering and its confrontation, so that students can get to know the services and resources and identify strengthening practices; 4) to continuously monitor and assess the mental health needs of students in health care courses. CONCLUSIONS The options are challenging and require universities to establish institutional commissions to implement a policy to strengthen the mental health of university students in the health area, with the ability to recognize the different health needs, including manifestations of psychic suffering ; to integrate the university’s internal actions with each other and with the services of the Unified Health System; to implement and monitor the actions that make up the mental health policy.

Abstract Eukaryotic cells have different mechanisms of post-transcriptional regulation. Among these mechanisms, microRNAs promote regulation of targets by cleavage or degradation of the mRNA. Fungi of the Paracoccidioides complex are the etiological agents of the main systemic mycosis of Latin America. These fungi present a plasticity to adapt and survive in different conditions, and the presence of microRNAs-like molecules could be part of the mechanisms that provide such plasticity. MicroRNAs produced by the host influence the progression of this mycosis in the lungs besides regulating targets involved in apoptosis in macrophage, activation of T and B cells and the production of cytokines. Therefore, this work analyzed the presence of regions in the genome of this fungus with a potential to encode microRNAs-like molecules. Here we show by analysis of sequence similarity the presence of 18 regions, putatively coding for microRNAs-like molecules in the Paracoccidioides brasiliensis genome. We also described the conservation of dicer and argonaut proteins and the cognate transcripts induced in the yeast parasitic phase. This work represents a starting point for the analysis of the presence of those molecules in the morphological stages of the fungus and their role in fungal development.

Abstract Carbonic anhydrases (CA) belong to the family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. In the present work, we characterized the cDNAs of four Paracoccidioides CAs (CA1, CA2, CA3, and CA4). In the presence of CO2, there was not a significant increase in fungal ca1, ca2 and ca4 gene expression. The ca1 transcript was induced during the mycelium-to-yeast transition, while ca2 and ca4 gene expression was much higher in yeast cells, when compared to mycelium and mycelium-to-yeast transition. The ca1 transcript was induced in yeast cells recovered directly from liver and spleen of infected mice, while transcripts for ca2 and ca4 were down-regulated. Recombinant CA1 (rCA1) and CA4 (rCA4), with 33 kDa and 32 kDa respectively, were obtained from bacteria. The enzymes rCA1 (β-class) and rCA4 (α-class) were characterized regarding pH, temperature, ions and amino acids addition influence. Both enzymes were stable at pHs 7.5-8.5 and temperatures of 30-35 °C. The enzymes were dramatically inhibited by Hg+2 and activated by Zn+2, while only rCA4 was stimulated by Fe2+. Among the amino acids tested (all in L configuration), arginine, lysine, tryptophan and histidine enhanced residual activity of rCA1 and rCA4.

Buscou-se identificar e analisar as representações sociais de monitores do PET-Saúde da Universidade Federal de Minas Gerais (UFMG), Brasil, que atuam na linha de pesquisa Saúde na Escola, concernente à influência dessa vivência na sua formação e no seu olhar sobre o trabalho em equipe interdisciplinar. O trajeto metodológico delineou as representações sociais dos monitores após vivenciarem o projeto. As respostas dos relatórios dos monitores foram digitalizadas e importadas para o programa NVivo10, categorizadas e analisadas por meio do discurso do sujeito coletivo. Levantaram-se dados de 33 monitores do PET-Saúde na Escola, que constituíram 16 componentes representacionais positivamente relacionados a sua formação (n=9) e ao trabalho em equipe interdisciplinar (n=7). Verificou-se que a vivência no referido projeto promoveu diferenciais relevantes na formação desses futuros profissionais de saúde, com percepções favoráveis ao trabalho em equipe interdisciplinar, atentas às demandas sociais do Sistema Único de Saúde (SUS).

This study aimed to identify and analyze social representations of PET-Health monitors at the Minas Gerais Federal University (UFMG), Brazil, which participate in the School Healthcare research area, with respect to the influence of this experience on their professional education and their outlook toward interdisciplinary teamwork. The methodology sought to delineate their social representations. Their responses were assessed, entered into a database, and imported to the NVivo.10 software. The data were categorized and evaluated using collective subject discourse analysis. Data from 33 monitors of PET-Saúde were gathered, and these formed 16 representational components: nine related to the monitors’ education and seven related to interdisciplinary teamwork. The experience made a relevant difference to the education of professionals, with favorable perceptions toward interdisciplinary teamwork focusing on the social demands of the Brazilian Health System (SUS).

El objetivo fue delinear las representaciones sociales de los monitores del PET-Salud-UFMG, Brasil, que actúan en la línea de investigación Salud en la Escuela, acerca de la influencia de esa experiencia en su formación y con respecto al trabajo en equipo interdisciplinario. El trayecto metodológico buscó delinear las representaciones sociales de los monitores. Fueron depuradas las respuestas en los informes de los monitores, digitalizadas e importadas para el programa NVivo.10. Posteriormente, fueron categorizadas y analizadas vía discurso del sujeto colectivo. Se pesquisaron datos de 33 monitores del PET-Salud en la Escuela, constituyendo 16 representaciones sociales, siendo nueve relacionadas a su formación y siete al trabajo en equipo interdisciplinario. La experiencia en el proyecto ayudó a promover diferencias para la formación de futuros profesionales de salud, con percepciones favorables para el trabajo en equipo interdisciplinario, dirigidas a las demandas sociales del Sistema Brasileño de Salud (SUS).

Buscou-se identificar e analisar as representações sociais de monitores do PET-Saúde da Universidade Federal de Minas Gerais (UFMG), Brasil, que atuam na linha de pesquisa Saúde na Escola, concernente à influência dessa vivência na sua formação e no seu olhar sobre o trabalho em equipe interdisciplinar. O trajeto metodológico delineou as representações sociais dos monitores após vivenciarem o projeto. As respostas dos relatórios dos monitores foram digitalizadas e importadas para o programa NVivo10, categorizadas e analisadas por meio do discurso do sujeito coletivo. Levantaram-se dados de 33 monitores do PET-Saúde na Escola, que constituíram 16 componentes representacionais positivamente relacionados a sua formação (n=9) e ao trabalho em equipe interdisciplinar (n=7). Verificou-se que a vivência no referido projeto promoveu diferenciais relevantes na formação desses futuros profissionais de saúde, com percepções favoráveis ao trabalho em equipe interdisciplinar, atentas às demandas sociais do Sistema Único de Saúde (SUS).

This study aimed to identify and analyze social representations of PET-Health monitors at the Minas Gerais Federal University (UFMG), Brazil, which participate in the School Healthcare research area, with respect to the influence of this experience on their professional education and their outlook toward interdisciplinary teamwork. The methodology sought to delineate their social representations. Their responses were assessed, entered into a database, and imported to the NVivo.10 software. The data were categorized and evaluated using collective subject discourse analysis. Data from 33 monitors of PET-Saúde were gathered, and these formed 16 representational components: nine related to the monitors’ education and seven related to interdisciplinary teamwork. The experience made a relevant difference to the education of professionals, with favorable perceptions toward interdisciplinary teamwork focusing on the social demands of the Brazilian Health System (SUS).

El objetivo fue delinear las representaciones sociales de los monitores del PET-Salud-UFMG, Brasil, que actúan en la línea de investigación Salud en la Escuela, acerca de la influencia de esa experiencia en su formación y con respecto al trabajo en equipo interdisciplinario. El trayecto metodológico buscó delinear las representaciones sociales de los monitores. Fueron depuradas las respuestas en los informes de los monitores, digitalizadas e importadas para el programa NVivo.10. Posteriormente, fueron categorizadas y analizadas vía discurso del sujeto colectivo. Se pesquisaron datos de 33 monitores del PET-Salud en la Escuela, constituyendo 16 representaciones sociales, siendo nueve relacionadas a su formación y siete al trabajo en equipo interdisciplinario. La experiencia en el proyecto ayudó a promover diferencias para la formación de futuros profesionales de salud, con percepciones favorables para el trabajo en equipo interdisciplinario, dirigidas a las demandas sociales del Sistema Brasileño de Salud (SUS).

The aim of this work was the partial purification and subsequent evaluation of chitinase expression during the various growth phases of Paracoccidioides brasiliensis. Initially, PbCTS1r was expressed as a recombinant protein and displayed enzymatic activity against 4-MU-[N-acetylglucosamine (GlcNAc)]3 and 4-MU-(GlcNAc)2. Two proteins, 45 kDa and 39 kDa in size, were partially purified from P. brasiliensis yeast crude extract using cation-exchange chromatography coupled with HPLC and were characterised as PbCTS1 and PbCTS2, respectively. Anti-PbCTS1r antibody recognised two proteins in the crude extracts of yeast and the transitional stage between mycelial and yeast phases. In crude extracts of mycelium, only the 45 kDa protein was detected. However, quantitative real-time polymerase chain reaction led to the detection of small quantities of Pbcts2 transcript in the mycelial phase. In the yeast cell wall extract, only the 39 kDa protein was detected. Moreover, both proteins were secreted by the yeast parasitic phase, suggesting that these proteins participate in the modulation of the fungal environment. Phylogenetic analysis of the predicted PbCTS1 and PbCTS2 proteins indicated that they code for distinct chitinases in P. brasiliensis. During evolution, P. brasiliensis could have acquired the paralogues Pbcts1 and Pbcts2 for growth and survival in diverse environments in both saprophytic and parasitic phases.

O gênero Babesia compreende protozoários causadores de enfermidades denominadas babesioses. Cães geralmente são acometidos por Babesia canis ou Babesia gibsoni, sendo a primeira classificada em subespécies Babesia canis canis, Babesia canis vogeli e Babesia canis rossi. Entre essas, Babesia canis vogeli predomina no Brasil. O objetivo desse trabalho foi realizar estudo filogenético de amostras de Babesia em cães, em Goiânia, Goiás. Amostras de sangue foram obtidas de 890 cães atendidos no Hospital Veterinário de Goiás, apresentando sinais clínicos de babesiose. Somente amostras com presença de parasitos intraeritrocitários típicos foram utilizadas. Estas foram submetidas a extração de DNA e amplificação de fragmento do gene 18S rRNA pela PCR. Os produtos de PCR foram purificados e sequenciados. Foram sequenciadas 35 amostras, das quais apenas 17 foram mantidas após avaliação de qualidade. A análise de similaridade fornecida pelo BLASTn demonstrou que as 17 sequências deste estudo eram correspondentes a Babesia canis vogeli. Pela utilização do programa Mega4, foi possível verificar que as amostras de Babesia canis vogeli, provenientes de cães da cidade de Goiânia, apresentam, alto grau de similaridade molecular (99,2 a 100%) com isolados de referência de outras regiões do Brasil e do mundo, depositados em GenBank.

The genus Babesia comprises protozoa that cause diseases known as babesiosis. Dogs are commonly affected by Babesia canis or Babesia gibsoni. Babesia canis is divided into the subspecies Babesia canis canis, Babesia canis vogeli and Babesia canis rossi. Among these, Babesia canis vogeli predominates in Brazil. The objective of this study was to conduct a phylogenetic analysis on Babesia isolates from dogs in Goiânia, Goiás. Blood samples were obtained from 890 dogs presenting clinical signs suggestive of canine babesiosis that were attended at a veterinary hospital of Goiás. Only samples presenting typical intraerythrocytic parasites were used in the study. These were subjected to DNA extraction and amplification of a fragment of the 18S rRNA, by means of PCR. The PCR products were purified and sequenced. Sequences were obtained from 35 samples but only 17 of these were kept after quality assessment. Similarity analysis using BLASTn demonstrated that all 17 sequences corresponded to B. canis vogeli. Analysis using the Mega4 software showed that the isolates of B. canis vogeli from dogs in Goiânia present a high degree of molecular similarity (99.2 to 100%) in comparison with other reference isolates from other regions of Brazil and worldwide, deposited in GenBank.

Paracoccidioides brasiliensis causes infection through inhalation by the host of airborne propagules from the mycelium phase of the fungus. This fungus reaches the lungs, differentiates into the yeast form and is then disseminated to virtually all parts of the body. Here we review the identification of differentially-expressed genes in host-interaction conditions. These genes were identified by analyzing expressed sequence tags (ESTs) from P. brasiliensis cDNA libraries. The P. brasiliensis was recovered from infected mouse liver as well as from fungal yeast cells incubated in human blood and plasma, mimicking fungal dissemination to organs and tissues and sites of infection with inflammation, respectively. In addition, ESTs from a cDNA library of P. brasiliensis mycelium undergoing the transition to yeast were previously analyzed. Together, these studies reveal significant changes in the expression of a number of genes of potential importance in the host-fungus interaction. In addition, the unique and divergent representation of transcripts when the cDNA libraries are compared suggests differential gene expression in response to specific niches in the host. This analysis of gene expression patterns provides details about host-pathogen interactions and peculiarities of sites within the host.

Hepatitis A virus (HAV) infection is a public health problem worldwide and the virus has been classified into six genotypes. In Brazil, the only genotype that has been found is genotype I, predominately from subgenotype IA. Here, the HAV genotypes were analyzed of 18 isolates circulating between 1996-2001 in Goiânia, state of Goiás, Brazil. Viral RNA was extracted from 18 serum samples and amplified (RT-PCR/nested-PCR), followed by the genomic sequencing of the VP1/2A junction region of the HAV genome. Sequences of 168 nucleotides were compared and analyzed using the BLAST N, Clustal X and PAUP v. 4.10b programs. All samples were classified as genotype I, with 10 belonging to subgenotype IA and eight to subgenotype IB. The subgenotype IA isolates showed greater diversity than the subgenotype IB isolates at the nucleotide level. Elevated identity values were found between isolates obtained in this study and those from other regions of the world, including Brazil, highlighting the high conservation among different isolates of this virus. However, changes in the HAV subgenotype circulation could also be observed during the evaluated period.

Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development.

Mycoplasmas are the smallest known prokaryotes with self-replication ability. They are obligate parasites, taking up many molecules of their hosts and acting as pathogens in men, animals, birds and plants. Mycoplasma hyopneumoniae is the infective agent of swine mycoplasmosis and Mycoplasma synoviae is responsible for subclinical upper respiratory infections that may result in airsacculitis and synovitis in chickens and turkeys. These highly infectious organisms present a worldwide distribution and are responsible for major economic problems. Proteins of the GTPase superfamily occur in all domains of life, regulating functions such as protein synthesis, cell cycle and differentiation. Despite their functional diversity, all GTPases are believed to have evolved from a single common ancestor. In this work we have identified mycoplasma GTPases by searching the complete genome databases of Mycoplasma synoviae and Mycoplasma hyopneumoniae, J (non-pathogenic) and 7448 (pathogenic) strains. Fifteen ORFs encoding predicted GTPases were found in M. synoviae and in the two strains of M. hyopneumoniae. Searches for conserved G domains in GTPases were performed and the sequences were classified into families. The GTPase phylogenetic analysis showed that the subfamilies were well resolved into clades. The presence of GTPases in the three strains suggests the importance of GTPases in 'minimalist' genomes.

Mycoplasma synoviae and Mycoplasma hyopneumoniae are wall-less eubacteria belonging to the class of Mollicutes. These prokaryotes have a reduced genome size and reduced biosynthetic machinery. They cause great losses in animal production. M. synoviae is responsible for an upper respiratory tract disease of chickens and turkeys. M. hyopneumoniae is the causative agent of enzootic pneumonia in pigs. The complete genomes of these organisms showed 17 ORFs encoding kinases in M. synoviae and 15 in each of the M. hyopneumoniae strain. Four kinase genes were restricted to the avian pathogen while three were specific to the pig pathogen when compared to each other. All deduced kinases found in the non pathogenic strain (J[ATCC25934]) were also found in the pathogenic M. hyopneumoniae strain. The enzymes were classified in nine families composing five fold groups.

This is a report on the analysis of genes involved in translation of the complete genomes of Mycoplasma hyopneumoniae strain J and 7448 and Mycoplasma synoviae. In both genomes 31 ORFs encoding large ribosomal subunit proteins and 19 ORFs encoding small ribosomal subunit proteins were found. Ten ribosomal protein gene clusters encoding 42 ribosomal proteins were found in M. synoviae, while 8 clusters encoding 39 ribosomal proteins were found in both M. hyopneumoniae strains. The L33 gene of the M. hyopneumoniae strain 7448 presented two copies in different locations. The genes encoding initiation factors (IF-1, IF-2 and IF-3), elongation factors (EF-G, EF-Tu, EF-Ts and EF-P), and the genes encoding the ribosome recycling factor (frr) and one polypeptide release factor (prfA) were present in the genomes of M. hyopneumoniae and M. synoviae. Nineteen aminoacyl-tRNA synthases had been previously identified in both mycoplasmas. In the two strains of M. hyopneumoniae, J and 7448, only one set of 5S, 16S and 23S rRNAs had been identified. Two sets of 16S and 23S rRNA genes and three sets of 5S rRNA genes had been identified in the M. synoviae genome.