ABSTRACT Lead is a heavy metal and an important cause of acute or chronic toxicosis in humans, domestic, and wild animals. This report aims to describe a case of chronic lead poisoning in a jaguar (Panthera onca) kept under human care that was rescued from the wild environment. The animal was rescued in poor condition in 2004 and kept under human care at the Belo Horizonte Zoological Garden (Minas Gerais, Brazil) until 2020, when it presented with anorexia, vomiting and ataxia. Over the past years the animal had episodes of anemia and increased serum urea and creatinine. Radiography demonstrated 21 radiopaque projectiles on the right side of the face. At necropsy there were multiple projectiles surrounded by fibrous tissue in the subcutaneous of the right side of the face, fibrinous peritonitis, multiple gastric ulcers, and melena. The lead dosage was performed using the atomic absorption spectrometry technique using renal tissue collected at necropsy, with a result of 908 ppb (µg/kg). The findings of projectiles associated with the dosage of lead above the reference limits allow the diagnosis of chronic intoxication in this case.

RESUMO Chumbo é um metal pesado e uma causa importante de toxicose crônica no homem e em animais domésticos e selvagens. Este relato teve como objetivo descrever um caso de intoxicação crônica por chumbo em uma onça-pintada (Panthera onca), mantida sob cuidados humanos, que foi resgatada de seu ambiente natural em 2004, em pobre condição corporal. O animal foi encaminhado ao zoológico de Belo Horizonte (MG, Brasil), onde permaneceu até 2020, quando desenvolveu anorexia, vômitos e ataxia. Durante os últimos anos, o animal teve episódios de anemia e aumento da concentração sérica de ureia e creatinina. O exame radiográfico demonstrou 21 projéteis radiodensos na face direita. À necropsia, foram observados vários projéteis envoltos por tecido conjuntivo no subcutâneo da face esquerda, peritonite fibrinosa, múltiplas úlceras gástricas e melena. A concentração de chumbo foi determinada por espectrometria de absorção atômica em amostras de tecido renal, indicando 908 ppb (µg/kg). O achado de projéteis de chumbo associado à elevada concentração de chumbo é compatível com intoxicação crônica por chumbo neste caso.

RESUMO Este estudo avaliou os aspectos clínicos e produtivos de matrizes Nelore, suplementadas ou não com P. Noventa, e cinco nulíparas prenhes foram distribuídas em dois grupos (G40 e G0). Os animais do G40 receberam uma mistura mineral com 224g de Na e 40g de P/kg, até a data do parto, e o G0 recebeu apenas NaCl. Após o parto, as primíparas foram divididas em quatro grupos: do G40, 28 continuaram a receber a mistura contendo 224g de Na e 40g de P/kg (grupo 40/40), e 12 passaram a receber apenas NaCl (grupo 40/0); do G0, 26 continuaram recebendo apenas NaCl (grupo 0/0), e 15 passaram a ser suplementadas com a mistura contendo 224g de Na e 40g de P/kg (grupo 0/40). Na segunda fase experimental, 205 vacas foram divididas em dois grupos: G1 (40/40), composto por 40 vacas, suplementadas com a mistura mineral contendo 244g de Na e 40g de P/kg, durante a gestação e a lactação, e o G2 (0/0) foi formado por 165 vacas, as quais foram suplementadas apenas com NaCl, durante a gestação e a lactação. Em ambos os experimentos, seja nas matrizes em gestação ou naquelas em lactação, não houve qualquer sinal da deficiência de P, e isso se refletiu na ausência de diferenças nos pesos dos bezerros aos 120 e 210 dias.

ABSTRACT The aim of this case study was to evaluate the clinical and productive data of Nellore cows during pregnancy and lactation, supplemented or not with P. Ninety-five pregnant heifers were divided into two groups (G40 and G0). Until the date of calving animals from G40 received a mineral mixture composed of 224g of Na and 40g of P/kg, and G0 received only NaCl. After calving heifers were divided into four treatments: from G40, 28 first-calf cows continued to receive the mineral mixture containing 224g of Na and 40g of P/kg (group 40/40) and 12 started to receive only NaCl (group 40/0); from G0, 26 continued to receive only NaCl (group 0/0) and 15 started to receive a mixture containing 224g of Na and 40g of P/kg (group 0/40). In the second experimental phase, 205 multiparous cows were divided into two groups: G1 (40/40) consisting of 40 cows, receiving a mineral mixture containing 244g of Na and 40g/P per kg, during pregnancy and lactation, and group 2 (0/0) formed by 165 cows, which were supplemented only with NaCl. In both experiments, whether in pregnant or lactating breeders, there was not any sign of P deficiency or differences in calves LW at 120 and 210 days.

Abstract Pediculosis mainly affects school-age children worldwide. The aim of this study was to identify and analyze the knowledge of the parents and guardians of children in elementary schools in Niterói, Brazil, regarding pediculosis. Questionnaires were applied to 237 guardians of children at five 1-5 grade municipal schools. The responses were analyzed and correlated with positivity to louse infestation, detected by scalp aspiration. 73.8% of the respondents reported that their child had already been infested with lice. 32.9% presented correct responses about transmission. Incorrect responses were attributed to the air/wind, blood type and the fact that lice jump and fly. 40.1% of the respondents erroneously correlated control over the parasitosis with hygiene. A majority of the participants (58.6%) responded that pediculosis is harmful to health, while a small proportion (20.7%) considered it to be a disease. The prevalence of pediculosis was 19.8% among schoolchildren. Female sex, pruritus on the head and indifference regarding infestation were shown to be risk factors for pediculosis. The lack of perception of pediculosis as a disease may lead to naturalization of this parasitosis. Incorrect responses may add difficulty to implementation of preventive and curative approaches, which highlights the importance of dissemination of correct information about pediculosis.

Abstract Liqueur is an alcoholic beverage composed of a mixture of water, alcohol, sugar and substances that add flavour and aroma. Wild passion fruit is a product with good agricultural and nutritional characteristics, and is a low-cost, regional fruit that could be used to elaborate new products. The goal of this study was to develop passion fruit (Passiflora cincinnata Mast.) liqueurs and evaluate their chemical, physical and sensory characteristics. 5 formulations were prepared with defined pulp and syrup concentrations (F1, F2, F3, F4 and F5). The following physicochemical parameters were evaluated: alcohol degree (ºGL), density, pH, total titratable acidity, total soluble solids (TSS), reducing and non-reducing sugars and colour parameters. A sensory acceptance test was applied. The formulations F4 and F5, produced with 640g pulp/70ºBrix syrup and 500g pulp/55ºBrix syrup, respectively, showed the highest acceptance scores, probably due to their acid pH, high acidity and soluble solids values. In general, the beverages developed were considered feasible, aimed at aggregating value to a regional fruit and increasing family incomes. The high sensory acceptance indicated market potential for this aggregated value product.

Abstract In 2019 an oil spill spread over more than 4000 km of the Brazilian coastline. Monitoring programs that allow for the evaluation of potential impacts on communities and ecosystems and their recovery through time are needed and rely on baseline information previous to the oil spill. Here we compiled qualitative and quantitative data available from 8 studies on fish and benthic species from different habitats of Rio Grande do Norte (RN) from 2007 to 2018. We assessed the number of species from different locations and compiled information on conservation status and human use. We mapped the study areas and habitats and overlapped them with those affected by the oil spill. The RN state has a high diversity of fish (175 species) and benthic species (285 species), of which more than half of fish (52%) and 2% of benthic species are threatened and 72,8% of fish and 7,9% of benthic species are of commercial interest. Information of most habitats is still poorly known (i.e.majority ofthe available data are from unique and punctual sampling), which may weaken future evaluations of the real impact of the oil spill. Nonetheless, it is possible to design reliable monitoring based on our dataset, persistent contamination and future loss of diversity.

RESUMO O teratoma é uma neoplasia rara, com diferenciação em duas ou três linhagens de células germinativas. Teratomas intracranianos, em aves, são raramente relatados, principalmente, com localização cerebral. Este é o primeiro relato de teratoma cerebral em um pato-real com sinais clínicos neurológicos. A neoplasia foi caracterizada como um teratoma cerebral maduro, estendendo do cerebelo ao tronco encefálico e com um nódulo no córtex.

ABSTRACT Teratoma is a rare neoplasia with differentiation in two or three germ cell lines. Intracranial teratoma in birds has rarely been reported, especially affecting the brain. This is the first report of a brain teratoma in a mallard with neurological clinical signs. The neoplasm was characterized as a mature brain teratoma, extending from the cerebellum to the brainstem, and with one nodule in the cortex.

The consumption of alcoholic beverages influences carbohydrate and lipid metabolism, although it is not yet clear whether metabolism during physical exercise at different intensities is also affected. This was the objective of the present study. Eight young and healthy volunteers performed a treadmill test to identify the running speed corresponding to a lactate concentration of 4 mM (S4mM). At least 48 h later, they were subjected to two experimental trials (non-alcohol or alcohol) in which they performed two 1-km running sessions at the following intensities: 1) S4mM; 2) 15% above S4mM. In both trials, blood lactate, triglycerides, and glucose concentrations were measured before and after exercise. The acute alcohol intake increased triglycerides, but not lactate concentration under resting conditions. Interestingly, alcohol intake enhanced the exercise-induced increase in lactate concentration at the two intensities: S4mM (non-alcohol: 4.2±0.3 mM vs alcohol: 4.8±0.9 mM; P=0.003) and 15% above S4mM trial (P=0.004). When volunteers ingested alcohol, triglycerides concentration remained increased after treadmill running (e.g., at S4mM - at rest; non-alcohol: 0.2±0.5 mM vs alcohol: 1.3±1.3 mM; P=0.048). In contrast, glucose concentration was not modified by either alcohol intake, exercise, or their combination. We concluded that an acute alcohol intake changed lactate and lipid metabolism without affecting blood glucose concentration. In addition, the increase in lactate concentration caused by alcohol was specifically observed when individuals exercised, whereas augmented triglycerides concentration was already observed before exercise and was sustained thereafter.

RESUMO Avaliou-se a influência da somatotropina recombinante bovina (rbST) sobre os metabolismos energético e mineral de búfalas entre 63e 154 dias em lactação. Foram utilizadas 22 búfalas, distribuídas em dois grupos experimentais: grupo rbST - aplicação de 500mg de rbST a cada 14 dias; grupo Controle - sem aplicação de rbST. A cada sete dias, foram coletadas amostras de sangue para a determinação do perfil bioquímico e mensuraram-se a produção de leite e o escore de condição corporal dos animais. As médias dos parâmetros estudados para os grupos rbST e Controle foram, respectivamente: produção de leite (PL): 6,44kg vs. 6,68kg; escore de condição corporal-ECC (1-5): 3,51 vs. 3,57; glicose: 70,58 vs. 64,81mg/dL (P = 0,0003); colesterol: 132,38 vs. 133,40mg/dL; triglicérides: 29,18 vs. 28,32mg/dL; proteína total: 8,57 vs. 8,75g/dL; albumina: 3,47 vs. 3,60g/dL; ureia: 32,46 vs. 33,86mg/dL; creatinina: 1,27 vs. 1,39mg/dL; cálcio:10,25 vs. 10,73mg/dL; fósforo:5,76 vs. 5,62mg/dL; e magnésio:3,70 vs. 3,70mg/dL. O uso de 500mg de rbSTinfluenciou o metabolismo da glicose, porém não modificou a PL, o ECC e os níveis dos demais parâmetros metabólicos estudados.

ABSTRACT The aim was to evaluate the influence of recombinant bovine somatotropin (rbST) on the energy and mineral metabolism of buffaloes between 63 - 154 days in milk. Twenty-two buffaloes distributed in two experimental groups were used: Group rbST (n= 11) - application of 500mg of rbST every 14 days; Control Group (n= 11) - no rbST. Every seven days, blood samples were taken to determine the biochemical profile, and milk production and body condition score were measured. The averages of the variables for rbST and Control groups were, respectively: milk yield (MY) - 6.44kg vs. 6.68kg; body condition score (BCS) - 3.51 vs 3.57 (1-5); glucose - 70.58 vs. 64.81mg/dL (P = 0.0003); cholesterol - 132.38 vs. 133.40mg/dL; triglycerides -29.18 vs. 28.32mg/dL; total protein - 8.57 vs. 8.75g/dL; albumin - 3.47 vs 3.60g/dL; urea - 32.46 vs 33.86mg/dL; creatinine - 1.27 vs 1.39mg/dL; calcium - 10.25 vs. 10.73mg/dL; phosphorus - 5.76 vs 5.62mg/dL; and magnesium - 3.70 vs 3.70mg/dL. Use of 500mg rbST influenced glucose metabolism, but did not modify the MY, BCS and the levels of the other metabolic parameters studied.

RESUMO Neste trabalho, foi avaliada a prevalência das principais causas de condenação de fígado bovino em abatedouro frigorífico sob Serviço de Inspeção Federal, situado na Zona da Mata mineira. Para obtenção dos dados, foram consultadas fichas relativas ao movimento mensal de abate de bovinos e de causas de condenação, no período compreendido entre 2007 e 2013, no referido estabelecimento. Durante esse período, foram abatidos 150.752 bovinos, e a prevalência de condenação hepática entre os anos variou de 9,38% a 14,09%. As principais patologias envolvidas foram abscesso, telangiectasia e fasciolose, responsáveis por 32,03%, 29,20% e 21,95% das condenações, respectivamente. Além disso, foi observada tendência de redução na frequência de condenação por abscesso (3,26%) e telangiectasia (0,68%) ao ano e, em contraposição, aumento por fasciolose de 3,40% ao ano. Conclui-se que as patologias responsáveis pelas condenações de fígado bovino foram abscesso, telangiectasia e fasciolose e que a inspeção post mortem tem grande importância no monitoramento da saúde e da produção animal e na qualidade da carne a ser comercializada.

ABSTRACT The aim of the present study was to evaluate the frequency of the main causes for condemnation of bovine liver in an abattoir/cold store under the Federal Inspection Services, located in the Zona da Mata of the state of Minas Gerais, between 2007 and 2013. Study data was collated from forms containing information about the number of cattle slaughtered per month and the causes for liver condemnation at the studied abattoir. During the period under study, 150,752 cattle were slaughtered, and the frequency of liver condemnation varied between 9.38% and 14.09% from year to year. The main pathologies that led to liver condemnation were abscesses, telangiectasis, and fasciolosis, which were responsible for 32.03%, 29.20%, and 21.95% of the condemnations, respectively. The frequency of condemnation because of abscesses and telangiectasis showed a trend of decline, 3.26% and 0.68% per year, respectively. The frequency of condemnation because of fasciolosis, however, showed a trend of increase by 3.40% per year. This study shows the usefulness of post mortem inspection of animal health and production monitoring.

O teste intradérmico para o diagnóstico da tuberculose bovina utiliza derivados proteicos purificados (PPD) de Mycobacterium bovis que são capazes de induzir reações de hipersensibilidade em animais infectados. No entanto, apresenta baixa especificidade devido à ocorrência de reações cruzadas com outras micobactérias. Neste sentido, o objetivo desse trabalho foi produzir proteínas recombinantes (ESAT-6, PE13, PE5 e ESX-1) de Mycobacterium bovis e avaliá-las como antígenos em teste intradérmico utilizando Cavia porcellus como modelo, e verificar se as condições empregadas na purificação (nativa ou desnaturante) interferem no desempenho antigênico dessas proteínas. As proteínas foram testadas em Cavia porcellus previamente sensibilizados com cepa M. bovis AN5 inativada, individualmente (160 µg) ou combinadas na forma de um coquetel (40 µg cada). O coquetel de proteínas induziu reações de hipersensibilidade nos animais sensibilizados significativamente superiores (p=0,002) as observadas nos animais não sensibilizados, possibilitando diferenciação. No entanto, as proteínas isoladamente não foram capazes de promover essa diferenciação. As condições de solubilização e purificação influenciaram o desempenho antigênico da proteína ESAT-6, pois, quando produzida em condição desnaturante desencadeou reações inespecíficas nos animais não sensibilizados, enquanto que aquela produzida em condições nativas e aplicada em concentrações de 6, 12, 24 e 48µg induziu reações significativas apenas nos animais sensibilizados, confirmando o seu potencial como antígeno.

The intradermal skin test for diagnosis of bovine tuberculosis has been used the purified protein derivative (PPD) of Mycobacterium bovis, that is able to induce a hypersensitivity reaction in infected animals. However, shows low specificity due to the occurrence of cross reactions with other mycobacteria. Thus, the aim of this study was to produce recombinant proteins (ESAT-6, PE13, PE5 and ESX-1) of Mycobacterium bovis and assess them as antigens in skin test using guinea pigs (Cavia porcellus) as a model, and check if the conditions employed in the purification (native or denaturing condition) interfere in the antigenic performance of these proteins. The proteins were tested in guinea pigs previously sensitized with inactivated M. bovis strain AN5, individually (160 µg/µl), or as a mixed cocktail (40 µg each). The cocktail of proteins induced hypersensitivity reactions in sensitized animals significantly (p=0.002) higher than those observed in non-sensitized animals, allowing differentiation. On the other hand, the proteins individually were not able to promote this differentiation. The conditions of solubilization and purification influenced the antigenic performance of the protein ESAT-6, since, when produced in denaturing condition triggered nonspecific reaction in non-sensitized animals. Whereas when produced under native conditions and used at concentrations (6, 12, 24 and 48µg/µl) induced a significant response only in sensitized animals, confirming its potential as antigen.

OBJECTIVES: Conflicting data from studies on the potential role of multidrug resistance 1 gene polymorphisms in inflammatory bowel disease may result from the analysis of genetically and geographically distinct populations. Here, we investigated whether multidrug resistance 1 gene polymorphisms are associated with inflammatory bowel diseases in patients from Rio de Janeiro. METHODS: We analyzed 123 Crohn's disease patients and 83 ulcerative colitis patients to determine the presence of the multidrug resistance 1 gene polymorphisms C1236T, G2677T and C3435T. In particular, the genotype frequencies of Crohn's disease and ulcerative colitis patients were analyzed. Genotype-phenotype associations with major clinical characteristics were established, and estimated risks were calculated for the mutations. RESULTS: No significant difference was observed in the genotype frequencies of the multidrug resistance 1 G2677T/A and C3435T polymorphisms between Crohn's disease and ulcerative colitis patients. In contrast, the C1236T polymorphism was significantly more common in Crohn's disease than in ulcerative colitis (p = 0.047). A significant association was also found between the multidrug resistance 1 C3435T polymorphism and the stricturing form of Crohn's disease (OR: 4.13; p = 0.009), whereas no association was found with penetrating behavior (OR: 0.33; p = 0.094). In Crohn's disease, a positive association was also found between the C3435T polymorphism and corticosteroid resistance/refractoriness (OR: 4.14; p = 0.010). However, no significant association was found between multidrug resistance 1 gene polymorphisms and UC subphenotypic categories. CONCLUSION: The multidrug resistance 1 gene polymorphism C3435T is associated with the stricturing phenotype and an inappropriate response to therapy in Crohn's disease. This association with Crohn's disease may support additional pathogenic roles for the multidrug resistance 1 gene in regulating gut-microbiota interactions and in mediating fibrosis. Understanding the effects of several drugs associated with multidrug resistance 1 gene variants may aid in the selection of customized therapeutic regimens.

Este artigo descreve a anteriormente desconhecida diversidade molecular de amostras brasileiras de Coronavírus canino (CCoV). Vinte e duas amostras foram submetidas à análise da sequência parcial do gene codificador da proteína de membrana, sendo 12 classificadas como CCoV Tipo II e 10 como CCoV Tipo I e uma possível sublinhagem tipicamente brasileira foi encontrada para o CCoV Tipo II.

Em estudos anteriores descrevemos o isolamento de nove substâncias, a partir das folhas de Derris urucu, uma espécie encontrada amplamente na floresta Amazônica, as quais foram identificadas como cinco estilbenos e quatro diidroflavonóis. Neste trabalho, três desses diidroflavonóis [urucuol A (1), urucuol B (2) e isotirumalina (3)] foram avaliados para identificar seus potenciais como aleloquímicos. Estamos relatando também, o isolamento e a determinação estrutural de um novo flavonóide [5,3′-diidroxi-4′-metoxi-(7,6:5″,6″)-2″,2″-dimetilpiranoflavanona (4)]. Investigamos os efeitos dos diidroflavonóis 1-3 sobre a germinação de sementes e desenvolvimento da radícula e do hipocótilo da planta daninha Mimosa pudica, usando soluções a 150 mg.L–1. Urucuol B, isoladamente, foi quem apresentou o maior potencial para inibir a germinação de sementes (26%), por sua vez, isotirulamina exibiu maior capacidade para reduzir o desenvolvimento do hipocótilo (25%), porém nenhuma das três substâncias mostrou potencial para inibir o desenvolvimento da radícula. Quando combinadas aos pares, as substâncias mostraram sinergismo ao desenvolvimento da radícula e do hipocótilo e efeitos, na germinação de sementes, que poderiam ser atribuídos a antagonismo. Quando testadas separadamente, as substâncias apresentaram maior tendência para inibir a germinação de sementes, enquanto que, quando testadas aos pares, observou-se aumento no efeito de inibição do desenvolvimento da planta daninha.

In some previous studies, we described the isolation of nine compounds from leaves of Derris urucu, a species found widely in the Amazon rainforest, identified as five stilbenes and four dihydroflavonols. In this work, three of these dihydroflavonols [urucuol A (1), urucuol B (2) and isotirumalin (3)] were evaluated to identify their potential as allelochemicals, and we are also reporting the isolation and structural determination of a new flavonoid [5,3′-dihydroxy-4′-methoxy-(7,6:5″,6″)-2″,2″-dimethylpyranoflavanone (4)]. We investigated the effects of the dihydroflavonols 1-3 on seed germination and radicle and hypocotyl growth of the weed Mimosa pudica, using solutions at 150 mg.L–1. Urucuol B, alone, was the substance with the greatest potential to inhibit seed germination (26%), while isotirumalin showed greater ability to reduce the development of the hypocotyl (25%), but none of the three substances showed the potential to inhibit radicle. When combined in pairs, the substances showed synergism for the development of root and hypocotyl and effects on seed germination that could be attributed to antagonism. When tested separately, the trend has become more intense effects on seed germination, while for the substances tested in pairs, the intensity of the effect was greater on development of weed.

A presença do limiar anaeróbio (LAn) em exercício resistido tem sido objeto de estudo nos últimos anos. O objetivo do estudo foi identificar e comparar o LAn em uma população idosa no leg press (LP) e supino reto (SR). Catorze homens idosos (68.9 ± 4.0 anos, 170.5 ± 0.05 cm e 76.1 ± 7.8 kg) realizaram dois testes incrementais máximos baseados em porcentagens de uma repetição máxima (1RM) para a determinação do LAn no LP e SR. As variáveis lactacidemia, frequência cardíaca (FC) e percepção subjetiva de esforço (PSE) foram coletadas ao final de cada estágio e comparadas entre os exercícios na intensidade referente ao LAn. O nível de significância adotado foi p = .05. O LAn no LP ocorreu em 27.9 ± 5.0% de 1RM, estatisticamente superior ao SR, que foi identificado em 21.5 ± 3.1% de 1RM. No entanto, a lactacidemia foi estatisticamente inferior no LP (1.36 ± 0.40 mmol.L-1) em relação ao SR (1.92 ± 0.38 mmol.L-1). Apesar de intensidades diferentes do LAn entre os exercícios, as variáveis FC e PSE não apresentaram diferenças significativas. Foi possível identificar o LAn em idosos nos aparelhos LP e SR, ocorrendo em intensidades inferiores no SR.

The presence of anaerobic threshold (AT) in resistance exercise has been studied in recent years. The aim of this study was to identify and to compare the AT in elderly population during leg press (LP) and bench press (BP). Fourteen elderly men (68.9 ± 4.0 years, 170.5 ± 0.05 cm e 76.1 ± 7.8 kg) performed two maximal incremental tests based on percentages of one repetition maximum (1RM) for the determination of AT during LP and BP. Blood lactate, heart rate (HR) and rating of perceived exertion (RPE) were measured at the final of each stage and it were compared between the exercises at the intensity of the AT. The level of significance was p = .05. The AT in the LP was 27.9 ± 5.0% of 1RM and in the BP was 21.5 ± 3.1% of 1RM, with statistical differences between the exercises. However, blood lactate was significantly lower on LP (1.36 ± 0.40 mmol.L-1) when compared with BP (1.92 ± 0.38 mmol.L-1). Although the intensity of AT has been different between the exercises, HR and RPE did not presented statistical significant differences. It was possible to identify the AT on elderly population during LP and BP exercises and it occurred in lower intensities for BP.

Neste trabalho estamos reportando o isolamento de cinco compostos de Aspergillus sp EJC08 isolado como endofítico de Bauhinia guianensis uma planta típica da Amazônia. Os compostos ergosterol (1), peróxido de ergosterol (2), mevalolactona (3), monometilsulocrina (4) e tripacidina A (5) foram isolados através de procedimentos cromatográficos e identificados por métodos espectrais de RMN 1D e 2D e EM. Os compostos 3, 4 e 5 foram testados contra Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis e Staphylococcus aureus e mostraram boa atividade.

The present work reports the isolation of five compounds from Aspergillus sp EJC08 isolated as endophytic from Bauhinia guianensis, a tipical plant of the Amazon. The compounds ergosterol (1), ergosterol peroxide (2), mevalolactone (3), monomethylsulochrin (4) and trypacidin A (5) were isolated by chromatographic procedures and identified by spectral methods of 1D and 2D NMR and MS. Compounds 3, 4 and 5 were tested against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus and showed good activity.