Pesquisa | SciELO

Abstract Hereditary transthyretin amyloidosis with peripheral neuropathy (ATTRv-PN) is an autosomal dominant inherited sensorimotor and autonomic polyneuropathy with over 130 pathogenic variants identified in the TTR gene. Hereditary transthyretin amyloidosis with peripheral neuropathy is a disabling, progressive and life-threatening genetic condition that leads to death in ~ 10 years if untreated. The prospects for ATTRv-PN have changed in the last decades, as it has become a treatable neuropathy. In addition to liver transplantation, initiated in 1990, there are now at least 3 drugs approved in many countries, including Brazil, and many more are being developed. The first Brazilian consensus on ATTRv-PN was held in the city of Fortaleza, Brazil, in June 2017. Given the new advances in the area over the last 5 years, the Peripheral Neuropathy Scientific Department of the Brazilian Academy of Neurology organized a second edition of the consensus. Each panelist was responsible for reviewing the literature and updating a section of the previous paper. Thereafter, the 18 panelists got together virtually after careful review of the draft, discussed each section of the text, and reached a consensus for the final version of the manuscript.

Resumo Polineuropatia amiloidótica familiar associada a transtirretina (ATTRv-PN) é uma polineuropatia sensitivo-motora e autonômica hereditária autossômica dominante com mais de 130 variantes patogênicas já identificadas no gene TTR. A ATTRv-PN é uma condição genética debilitante, progressiva e que ameaça a vida, levando à morte em ~ 10 anos se não for tratada. Nas últimas décadas, a ATTRv-PN se tornou uma neuropatia tratável. Além do transplante de fígado, iniciado em 1990, temos agora 3 medicamentos modificadores de doença aprovados em muitos países, incluindo o Brasil, e muitas outras medicações estão em desenvolvimento. O primeiro consenso brasileiro em ATTRv-PN foi realizado em Fortaleza em junho de 2017. Devido aos novos avanços nesta área nos últimos 5 anos, o Departamento Científico de Neuropatias Periféricas da Academia Brasileira de Neurologia organizou uma segunda edição do consenso. Cada panelista ficou responsável por rever a literatura e atualizar uma parte do manuscrito. Finalmente, os 18 panelistas se reuniram virtualmente após revisão da primeira versão, discutiram cada parte do artigo e chegaram a um consenso sobre a versão final do manuscrito.

https://doi.org/10.1055/s-0043-1764412

Spintherobolus papilliferus in the rio Ribeira de Iguape basin: implications for the biogeography and conservation of an endangered species (Ostariophysi: Characiformes)
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Mattox, George M. T.

; Cetra, Mauricio

; Souza, Camila S.

; Oyakawa, Osvaldo T.

; Bento, Rodrigo M. M.

; Oliveira, Claudio

Neotropical Ichthyology

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2023, Volume 21 Nº 2 elocation e230026

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Spintherobolus papilliferus is an endangered characid endemic of the Atlantic Rainforest, known from sparse locations in the upper rio Tietê basin around the metropolitan area of São Paulo city, and from an affluent of rio Itapanhaú, a coastal stream in Bertioga, São Paulo State. In 2020, S. papilliferus was sampled from the rio Ribeira de Iguape basin in Juquitiba, São Paulo State, representing a new distributional record. We compared 17 morphometric and six meristic characters from all specimens with data from the rios Tietê and Itapanhaú. An overlap in the morphological data from the three populations was detected, except for five measure whose values are lower in rio Ribeira de Iguape. MANOVA and LDA revealed that this population differs significantly from the other two, showing shallower body and caudal peduncle, among other features. These morphological differences may be due to environmental selective pressures since rio Ribeira de Iguape drainage is marked by fast waters which can influence the shape of fish bodies over time. DNA-barcoding of all Spintherobolus species corroborate that the rio Ribeira de Iguape population belongs to S. papilliferus. We also present a hypothesis for the disjunct distribution of S. papilliferus involving headwater capture and discuss the implications for the conservation of this endangered species.

Resumo Spintherobolus papilliferus é um caracídeo endêmico da Mata Atlântica e ameaçado de extinção, conhecido de localidades esparsas na bacia do alto rio Tietê, região metropolitana da cidade de São Paulo, e de um afluente do rio Itapanhaú, um riacho costeiro em Bertioga, Estado de São Paulo. Em 2020, S. papilliferus foi amostrada na bacia do rio Ribeira de Iguape em Juquitiba, São Paulo, representando um novo registro de distribuição. Comparamos 17 caracteres morfométricos e seis merísticos dos exemplares com dados dos rios Tietê e Itapanhaú. Houve sobreposição nos valores dos dados exceto em cinco medidas cujos valores são menores no rio Ribeira de Iguape. MANOVA e LDA revelaram que esta população difere significativamente das demais populações, sendo estes exemplares caracterizados pelas menores alturas do corpo e pedúnculo caudal, dentre outras características. Estas diferenças morfológicas possivelmente decorrem de pressões ambientais, pois a drenagem do rio Ribeira de Iguape possui ambientes de corredeiras velozes que podem influenciar no formato do corpo dos peixes. DNA-barcoding de todas as espécies de Spintherobolus corroborou que a população do rio Ribeira de Iguape pertence à S. papilliferus. Apresentamos também uma hipótese para a distribuição disjunta de S. papilliferus envolvendo captura de cabeceiras e discutimos implicações para a conservação dessa espécie ameaçada.

https://doi.org/10.1590/1982-0224-2023-0026

Perfil epidemiológico de pacientes com degeneração macular relacionada à idade
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Carvalho, Jamil Augusto

; Rodrigues, Fernando

; Oda, Fernanda M.

; Caldas, Juliana M. Oliveira

; Brandão, Camila

; Souza, Maria Augusta Ornelas de

; Fonseca, Maria Luiza Gois da

; Rocha, Felipe Bekman

; Damasceno, Nadyr Antonia

; Lima, Luiz Claudio Santos S.

; Damasceno, Eduardo F.

Revista Brasileira de Oftalmologia

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2023, Volume 82 elocation e0061

Resumo: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Purpose To demonstrate the epidemiological profile and risk factors of patients with age-related macular degeneration (ARMD). Methods Observational and sectional study. One eye of each patient with greater visual impairment due to AMD treated at the University Hospital Antônio Pedro was included. The main variable to be compared was the presence of AMD divided into inexpressive, initial, intermediate, and advanced stages, according to the classification of the AREDS study. The secondary variables were demographics (gender, age, race, age group), body mass index, iris color, family history of ARMD, lens status, long-term exposure to ultraviolet light and smoking. Statistical tests were performed with Kruskal-Wallis and Chi-square analyses and Student’s t test. The significance level was set at 5%. Results After the inclusion and exclusion criteria, 126 patients were included in this study, 20 patients with non-expressive AMD, 30 patients with early AMD, 30 patients with intermediate AMD, and 46 patients with advanced AMD. Among all the risk factors surveyed, only smoking was statistically significant (p = 0.03). Conclusion Smoking as a risk factor for AMD had significant relevance prevalent in this research and even as a preventive factor for this ocular morbidity.

RESUMO Objetivo Demonstrar o perfil epidemiológico e fatores de riscos de pacientes com degeneração macular relacionada à idade. Métodos Estudo observacional e seccional. Foi incluído um olho de cada paciente com maior comprometimento visual por degeneração macular relacionada à idade atendido no Hospital Universitário Antônio Pedro. A variável principal a ser comparada foi a presença de degeneração macular relacionada à idade dividida nos estágios inexpressivo, inicial, intermediário e avançado, segundo classificação do estudo AREDS. As variáveis secundárias foram os dados demográficos (sexo, idade, raça, faixa etária), índice de massa corporal, cor da íris, história familiar de degeneração macular relacionada à idade, status do cristalino, longo tempo exposição à luz ultravioleta e tabagismo. Foram realizados testes estatísticos com análise de Kruskal-Wallis, do teste do qui-quadrado e teste t de Student. O nível de significância foi definido em 5%. Resultados Após os critérios de inclusão e exclusão, 126 pacientes foram incluídos neste estudo, sendo 20 pacientes com degeneração macular relacionada à idade inexpressiva, 30 pacientes com degeneração macular relacionada à idade inicial, 30 pacientes com degeneração macular relacionada à idade intermediária e 46 pacientes com degeneração macular relacionada à idade avançada. Dentre todos os fatores de risco pesquisados, apenas o tabagismo se mostrou estatisticamente significativo (p=0,03). Conclusão O tabagismo como fator de risco para degeneração macular relacionada à idade apresentou ter importância expressiva prevalente nesta pesquisa e até mesmo como fator preventivo dessa morbidade ocular.

https://doi.org/10.37039/1982.8551.20230061

Deep sea ecosystem exploration and ‘health check’: sampling strategy and methods applied during the iAtlantic_BR10_Petrobras cruise in the Santos Basin, Southwest Atlantic
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Perez, Jose Angel A.

; Barros Neto, Halésio M.C. de

; Arantes, Renata C.M.

; Gaurisas, Daniela Y.

; Silva, Camila F.

; Alves, Fernanda Maria M.

; Costa, Júlia A.

; Eloi, Paula D.C.

; Fonseca, Thayse S.

; Gavazzoni, Lucas

; Lonskis, Isabella da S.

; Nardi, Ricardo U.

; Nascimento, Pedro O.

; Rodrigues, João Vitor M.

; Santos, Ana Lara F. dos

; Santos, Edson C. dos

; Schroeder, Rafael

; Silva, Luis Carlos da

; Souza, Fernanda dos S.S. de

; Bernardino, Ângelo F.

; Cavalcanti, Guarani H.

; Lindner, Alberto

; Mahiques, Michel M. de

; Millo, Christian

; Reis, Priscila

; Sweetman, Andrew K.

; Roberts, J. Murray

Ocean and Coastal Research

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2023, Volume 71 elocation e23046

Resumo: En | Texto: En | PDF: En

Abstract The iAtlantic Project has established an international collaborative strategy to improve mapping and characterization of deep and open ocean ecosystems in understudied regions of the Atlantic and evaluate their health. In December 2022, the first iAtlantic expedition in the South Atlantic set off to map and explore seafloor ecosystems in the Santos Basin slope (200-1,000 m depths) in collaboration with the Petrobras ‘Santos Basin - Regional Characterization Project.’ The 17-day ‘iAtlantic_BR10-Petrobras’ cruise was conducted on board the research vessel NPqHOc Vital de Oliveira (Brazilian Navy) and performed (a) water column structure characterization, (b) seafloor morphology mapping, (c) description of benthic habitats and communities by seafloor imagery and biological/ geological sampling, and (d) ex-situ experiments to assess the functioning of sedimentary ecosystems and their responses to climate-related environmental changes. This study describes the rationale behind the iAtlantic_BR10-Petrobras cruise science plan, reports its sampling strategy and methods, and summarizes its collected data and preliminary results.

https://doi.org/10.1590/2675-2824071.23069jaap

Adjustment of inoculum levels for the evaluation of carrot genotypes resistance to Meloidogyne incognita
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Magalhães, Caroline da C

; Carvalho, Agnaldo DF de

; Biscaia, Danielle

; Melo, Raphael Augusto de C e

; Souza, Ludmila R de

; Alves, Camila dos S

; Silva, Giovani Olegário da

; Pinheiro, Jadir B

Horticultura Brasileira

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Set 2022, Volume 40 Nº 3 Páginas 321 - 325

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Um dos principais fitopatógenos que causam prejuízos econômicos em diversas espécies agrícolas, inclusive cenoura, são os nematoides-das-galhas, pertencentes ao gênero Meloidogyne. Assim, com o objetivo de identificar o melhor nível populacional de inóculo de Meloidogyne incognita para a máxima expressão de sintomas do ataque de nematoides-das-galhas em cenoura, foi realizado um experimento que avaliou os genótipos de cenoura cv. Brasília (CBR) e a população Pop-750 (CRO). O experimento foi conduzido em casa de vegetação da Embrapa Hortaliças, Brasília-DF em vasos de 5 L, com seis repetições, em esquema fatorial 4x2 em delineamento inteiramente casualizado, nas dosagens de 0, 1, 5 e 10 mil ovos e eventuais juvenis de segundo estádio (O+J2R). O tomateiro suscetível ‘Rutgers’ foi utilizado como testemunha para verificar eficiência do inóculo. A inoculação foi realizada 30 dias após a semeadura e, a avaliação, 60 dias após a inoculação. Foi realizada a avaliação dos índices de galhas (IG) e de massas de ovos (IMO), do número de ovos mais eventuais juvenis de segundo estádio por grama de raiz (O+J2R) e do fator de reprodução (FR). Houve diferenças entre os genótipos e entre os níveis de inóculo para todas as variáveis avaliadas. Para CRO, os níveis de inóculo de M. incognita a partir de mil O+J2 as plantas já manifestaram sintomas e alterações para todas as variáveis avaliadas, com os níveis ideais em torno de 5 a 7 mil O+J2R, sendo que acima de 7 mil O+J2R a multiplicação do nematoide para expressar sintomas diminuiu. Para a CBR, as variáveis índices O+J2R na raiz e FR, os níveis de inóculo próximos a 5 mil O+J2R também apresentam os melhores resultados. Quando a caracterização for baseada na avaliação da formação de galha (IG) e massa de ovos (IMO), os níveis de inóculo adequados seriam entre 9 e 12 mil O+J2R.

ABSTRACT One of the main phytopathogens that cause enormous economic losses in several agricultural species, including carrots, are the root-knot nematodes belonging to the genus Meloidogyne. Thus, in order to identify the best population level for inoculum of the root-knot nematode (Meloidogyne incognita) for maximum expression of symptoms of this nematode attack on carrots, an experiment was carried out to evaluate the carrot cultivar Brasília (CBR) and the population Pop-750 (CRO). The experiment was carried out in a greenhouse at Embrapa Vegetable, Brasília-DF, in 5 L pots, with six replications, in a 4x2 factorial scheme in a completely randomized design, in the dosages of 0, 1, 5 and 10 thousand eggs and occasional juveniles of 2nd stage (E+J2R). ‘Rutgers’ susceptible tomato cultivar was used as control to verify inoculum efficiency. Inoculation was carried out 30 days after sowing and evaluation 60 days after inoculation. Gall index (GI), egg mass index (EMI), number of eggs plus occasional second stage juveniles per gram of root (E+J2R) and reproduction factor (RF) were performed. There were differences between genotypes and between inoculum levels for all variables evaluated. For CRO, inoculum levels of M. incognita from 1,000 E+J2 the plants already manifested symptoms and changes in all evaluated variables, with ideal levels around 5 to 7 thousand E+J2R, above 7 thousand E+J2R nematode multiplication to express symptoms decreased. For CBR the response variables E+J2 at root and RF inoculum levels close to 5 thousand E+J2R also present the best results, but when the characterization is based on the evaluation of GI and EMI, suitable inoculum levels would be close to 9 and 12 thousand E+J2R.

https://doi.org/10.1590/s0102-0536-20220311

Social school contacts of multibacillary leprosy cases in children living in the hyperendemic region of the Midwest of Brazil
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Sato, Camila Massae

; Rodrigues, Thaisa da Silva Vargas

; Silva, Pãmela Rodrigues de Souza

; Santos, Emerson Soares dos

; Xavier, Diego Ricardo

; Baptista, Ida Maria Foschiani Dias

; Cortela, Denise da Costa Boamorte

; Ignotti, Eliane

; Ferreira, Silvana Margarida Benevides

Jornal de Pediatria

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Jul 2022, Volume 98 Nº 4 Páginas 431 - 437

Resumo: En | Texto: En | PDF: En

Abstract Objective: To analyze the prevalence of Mycobacterium leprae detection and the associated factors among social contacts in the school environment of multibacillary cases living in a hyperendemic municipality of the state of Mato Grosso. Methods: Cross-sectional study with 236 social contacts of multibacillary leprosy from public schools and residents in Cuiabá (Mato Grosso) in 2018. The sources of information were interviews and nasal swab tests for molecular analysis by polymerase chain reaction - PCR. For the prevalence ratio estimates, crude and adjusted analyses were performed using robust Poisson regression and their respective confidence intervals (95% CI). The ArcGIS 9.1 software was used for the geographic distribution analyses. Results: The prevalence of detection of M. leprae in social contacts was 14%. A total of 63.6% of the schools surveyed had 5.1% to 50% of the social contacts of leprosy with positive PCR. The analysis of the geographic distribution in the neighborhoods showed a high prevalence of infection, being higher than 50% in some localities. The highest proportion of positive results occurred in the northern region of the city and from a precarious socioeconomic class. Conclusion: The results showed a high prevalence of detection of M. leprae among social contacts in areas with poor socioeconomic conditions. In these regions, there is a greater risk of

https://doi.org/10.1016/j.jped.2021.11.009

Official position of the Brazilian Association of Bone Assessment and Metabolism (ABRASSO) on the evaluation of body composition by densitometry: part I (technical aspects)— general concepts, indications, acquisition, and analysis
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Maeda, Sergio Setsuo

; Peters, Barbara Santarosa Emo

; Martini, Lígia Araújo

; Antunes, Hannah Karen Moreira

; Gonzalez, Maria Cristina

; Arantes, Henrique Pierotti

; Prado, Carla M.

; Pinto, Camila Lemos

; Araújo, Iana Mizumukai de

; Paula, Francisco José Albuquerque de

; Borges, Joao Lindolfo Cunha

; Albergaria, Ben-Hur

; Ushida, Marcela

; Souza, Guilherme Cardenaz de

; Mendonça, Laura Maria Carvalho de

; Prado, Mirley do

; Pinheiro, Marcelo de Medeiros

Advances in Rheumatology

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2022, Volume 62 elocation 7

Resumo: En | Texto: En | PDF: En

Abstract Objective: To review the technical aspects of body composition assessment by dual-energy X-ray absorptiometry (DXA) and other methods based on the most recent scientific evidence. Materials and methods: This Official Position is a result of efforts by the Scientific Committee of the Brazilian Association of Bone Assessment and Metabolism (Associação Brasileira de Avaliação Óssea e Osteometabolismo, ABRASSO) and health care professionals with expertise in body composition assessment who were invited to contribute to the preparation of this document. The authors searched current databases for relevant publications. In this first part of the Official Position, the authors discuss the different methods and parameters used for body composition assessment, general principles of DXA, and aspects of the acquisition and analysis of DXA scans. Conclusion: Considering aspects of accuracy, precision, cost, duration, and ability to evaluate all three compartments, DXA is considered the gold-standard method for body composition assessment, particularly for the evaluation of fat mass. In order to ensure reliable, adequate, and reproducible DXA reports, great attention is required regarding quality control procedures, preparation, removal of external artifacts, imaging acquisition, and data analysis and interpretation.

https://doi.org/10.1186/s42358-022-00241-8

RANDOM FOREST MODEL TO PREDICT THE HEIGHT OF EUCALYPTUS
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Lima, Elizeu de S.

; Souza, Zigomar M. de

; Oliveira, Stanley R. de M.

; Montanari, Rafael

; Farhate, Camila V. V.

Engenharia Agrícola

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2022, Volume 42 Nº spe elocation e20210153

Resumo: En | Texto: En | PDF: En

ABSTRACT Eucalyptus (Eucalyptus urograndis) production has significantly advanced over the past few years in Brazil, especially with regard to acreage and productivity. Machine learning has made significant advances in most varied fields of agrarian sciences. In this context, this study aimed to use physicochemical variables of the soil as well as climatic and dendrometric variables of eucalyptus to predict its height using the random forest algorithm. The study was conducted in the municipality of Três Lagoas, in Mato Grosso do Sul, Brazil. The original database consisted of 49 soil physicochemical variables collected at 0–0.20 m and 0.20–0.40 m, two dendrometric and four climatic variables, and one response variable related to the height of eucalyptus. A correlation matrix was applied to select variables. Furthermore, modeling was performed using the random forest algorithm, which performed well (r = 0.98, R2 = 0.96) in predicting the height of eucalyptus. Overall, the most important variables to predict the eucalyptus plant height included diameter at breast height (DBH), phosphorus content (P1), gravimetric moisture (GM1) at a soil depth between 0.00 m and 0.20 m, and exchangeable aluminum content (Al2) between 0.20 m to 0.40 m of soil.

https://doi.org/10.1590/1809-4430-eng.agric.v42nepe20210153/2022

Does [18F]F-FDG-PET/MRI add metabolic information to magnetic resonance image in childhood-onset Takayasu’s arteritis patients? A multicenter case series
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Clemente, Gleice

; Souza, Alexandre W. de

; Leão Filho, Hilton

; Coelho, Fernando M. A.

; Buchpiguel, Carlos

; Lima, Marcos

; Carneiro, Camila

; Pereira, Rosa M. R.

; Aikawa, Nadia

; Silva, Clovis A.

; Campos, Lucia M. A.

; Alves, Gabriel

; Astley, Camilla

; Gualano, Bruno

; Terreri, Maria Teresa

Advances in Rheumatology

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2022, Volume 62 elocation 28

Resumo: En | Texto: En | PDF: En

Abstract Background: The observation that 2-deoxy-2[18F]fluoro-D-glucose-positron emission tomography/magnetic resonance imaging ([18F]F-FDG-PET/MRI) revealed high-grade arterial wall FDG uptake, without arterial wall thickening with contrast-enhancement, in a considerable number of c-TA patients in our previous study, encouraged us to compare patients with both PET and MR angiography (MRA) positives, with those with PET positive but MRA negative. Our aim was to evaluate the relevance of these two imaging modalities together. Methods: A three-center cross-sectional study with 17 patients who fulfilled the EULAR/PRINTO/PReS criteria for c-TA and who underwent [18F]F-FDG-PET/MRI was previously performed. Herein we compared patients/vessels with positive PET (arterial wall 18F-FDG uptake higher than liver) and positive MRA (arterial wall thickening with contrast-enhancement)—group 1, with those with positive PET but negative MRA—group 2. Results: Median disease duration of 17 c-TA patients was 10.4 years. Nine patients were classified as group 1 and six as group 2. Median of metabolic inflammatory volume (MIV) of all arterial segments was significantly higher in group 1 (2346 vs. 1177 cm3; p = 0.036). Fifty-four (19%) from 284 available arterial segments presented positive findings in vessel wall in one or both images. Positive findings were concordant between PET and MRA in only 13% arterial segments (group 1); most changes (28–59.6%) that were discordant between both images, were positive in PET and negative in MRA (group 2). Conclusions: Our study demonstrated that [18F]F-FDG-PET/MRI added information about inflammation in vessel wall of c-TA patients. Prospective multicenter studies are needed in order to get solid data to guide immunosuppressive tapering and withdrawal.

https://doi.org/10.1186/s42358-022-00260-5

10.

Application of Binary PdSb/C as an Anode in a Polymeric Electrolyte Reactor-Fuel Cell Type for Electrosynthesis of Methanol from Methane
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Godoi, Camila M.

; Santos, Monique C.L.

; Nunes, Livia C.

; Silva, Araceli J.

; Ramos, Andrezza S.

; Souza, Rodrigo F.B. de

; O. Neto, Almir

Materials Research

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2022, Volume 25 elocation e20210542

Resumo: En | Texto: En | PDF: En

Abstract PdSb catalyst prepared in different compositions were applied as an anode in a polymeric electrolyte reactor - fuel cell type (PER-FC) to convert methane into oxygenated products and energy in mild conditions. The PER-FC polarization curves for Pd90Sb10/C presented maximum current density about 0.92 mW cm-2 about 15% higher than PdSb materials. However, the material Pd50Sb50/C showed higher reaction rate for methanol generation than the other materials occurring close to the OCV (r ~ 7 mol L-1 h-1). The qualitative analyses of PER-FC effluent by FT-IR identified products as methanol, carbonate and formate ions from the partial oxidation of methane for all materials.

https://doi.org/10.1590/1980-5373-mr-2021-0542

11.

ELSA-Brasil: a 4-year incidence of hearing loss in adults with and without hypertension
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Padilha, Fernanda Yasmin Odila Maestri Miguel

; Oenning, Nágila Soares Xavier

; Santos, Itamar de Souza

; Rabelo, Camila Maia

; Moreira, Renata Rodrigues

; Bensenor, Isabela M.

; Lotufo, Paulo A.

; Samelli, Alessandra Giannella

Revista de Saúde Pública

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2022, Volume 56 elocation 28

Resumo: En | Texto: En | PDF: En

ABSTRACT OBJECTIVE To compare the incidence of hearing loss among adults stratified by the occurrence of hypertension, and to investigate the association between hypertension and hearing loss. METHODS Longitudinal observational study, part of the Estudo Longitudinal da Saúde do Adulto (ELSA-Brasil, Longitudinal Study on Adult’s Health). Data from the first and second waves were analyzed, including information from audiological assessment and general health of the subjects. As outcome, we considered the presence of hearing loss (hearing thresholds above 25 dBHL at frequencies from 500 Hz to 8 kHz) and, as exposure variable, hypertension (report of medical diagnosis of hypertension; and/or use of drugs to treat hypertension; and/or pressure systolic blood pressure ≥ 140 mmHg; or diastolic blood pressure ≥ 90 mmHg). As covariables for adjustment were considered: sex, age, education, race / ethnicity, income, smoking, diabetes, and occupational exposure to noise. Poisson regression analysis was conducted, estimating the crude and adjusted relative risks, with 95% confidence intervals, in order to assess the factors associated with hearing loss. RESULTS In crude analyses, the incidence of hearing loss was higher for subjects with hypertension (9.7% versus 5.4%). The crude relative risks for hearing loss was almost double (1.93; 95%CI: 1.10–3.39) for subjects with hypertension in the right ear. In the adjusted analyses, the relative risks was not significant for the hypertension variable (1.42; 95%CI: 0.75–2.67). Being 60 years or older (RR: 5.41; 95%CI: 2.79–10.50) showed a statistically significant association with hearing loss, indicating that older adults have higher relative risks for hearing loss. CONCLUSION In the adjusted analyses controlled for multiple risk factors there was no association between hypertension and hearing loss. The dichotomous variable age (being 60 years or older), on the other hand, has shown a significant association with hearing loss.

https://doi.org/10.11606/s1518-8787.2022056003796

12.

ELSA-Brasil: a 4-year incidence of hearing loss in adults with and without hypertension
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Padilha, Fernanda Yasmin Odila Maestri Miguel

; Oenning, Nágila Soares Xavier

; Santos, Itamar de Souza

; Rabelo, Camila Maia

; Moreira, Renata Rodrigues

; Bensenor, Isabela M.

; Lotufo, Paulo A.

; Samelli, Alessandra Giannella

Revista de Saúde Pública

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2022, Volume 56 elocation 28

Resumo: En | Texto: En | PDF: En

https://doi.org/10.11606/s1518-8787.2022056003796

13.

A novel collagenolytic protease from Mucor subtilissimus UCP 1262: Comparative analysis of production and extraction in submerged and stated-solid fermentation
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SOUZA, KESSIA P.S.

; CUNHA, MÁRCIA N.C.

; BATISTA, JUANIZE M.S.

; OLIVEIRA, VAGNE M.

; NASCIMENTO, THIAGO P.

; CONNIFF, AMANDA E.S.

; COSTA, ROMERO M.P.B.

; PORTO, TATIANA S.

; PORTO, CAMILA S.

; PORTO, ANA LÚCIA F.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 4 elocation e20201438

Resumo: En | Texto: En | PDF: En

Abstract This work aimed to compare the production of collagenolytic proteases produced by M. subtilissimus UCP1262 in submerged fermentation (SF) and solid-state fermentation (SSF) as well as extracting in aqueous two-phase system (ATPS). Collagenolytic protease production was performed in using MS-2 culture medium (SF) and soybean bran as substrate (SSF). Subsequently, the fermented liquid from both fermentations were used for the extraction of enzyme by ATPS, it was verified the influence of different variables from a factorial design 23. In SSF the highest protease and collagenolytic activities were achieved with 362.66 U/mL and 179.81 U/mL, respectively. When compared with SF (26.33 and 18.70 U/mL) higher values were obtained in the activities. The protease partitioning from SF and SSF in ATPS showed a similar profile showing higher affinity for the polymer rich phase. The highest value for the response variable purification factor (3.49) was obtained in the system using SSF. Thus, SSF shows promise as a bioprocess for extracellular production of collagenolytic proteases, using of soybean bran as substrate had used sustainable raw material, aiming application this possible enzyme in the treatment of burns and postoperative scarring.

https://doi.org/10.1590/0001-3765202220201438

14.

Seep hunting in the Santos Basin, Southwest Atlantic: sampling strategy and employed methods of the multidisciplinary cruise BIOIL 1
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Sumida, Paulo Y. G.

; Pellizari, Vivian H.

; Lourenço, Rafael A.

; Signori, Camila N.

; Bendia, Amanda G.

; Carrerette, Orlemir

; Nakamura, Fernanda M.

; Ramos, Raissa B.

; Bergamo, Gilberto

; Souza, Bruno H. M.

; Butarelli, Ana C. A.

; Passos, Júlia G.

; Dias, Rodolfo J. S.

; Maly, Mascimiliano

; Banha, Thomás N. S.

; Güth, Arthur Z.

; Soares, Luiza F.

; Perugino, Paula D. N.

; Santos, Felipe R.

; Santana, Frederico R.

; Mahiques, Michel M. de

Ocean and Coastal Research

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2022, Volume 70 Supl. 2 elocation e22031

Resumo: En | Texto: En | PDF: En

Abstract The continental margin off the southeastern Brazilian coast is punctuated by a series of geological-geomorphological features, such as subsurface saline diapirs and pockmarks at the seafloor interface, which evidence the abundant presence of oil and gas in the region. In several of these sites, hydrocarbons can be naturally released into the water column, areas are cold seep areas. These are marked by the presence of oil- and gas-dependent ecosystems, where specific organisms are able to fix carbon from hydrocarbon chemosynthesis. In addition, light hydrocarbon fluid flow through the sediment may build up authigenic carbonates that can be further colonized by cold-water corals, generating large carbonate mounds over geological time, normally positioned at the border of these pockmark features. The present work reports on a multidisciplinary oceanographic cruise carried out in the Santos Basin, SW Atlantic, to seek, map, and collect geological, chemical, and biological data from different deep-sea habitats. The cruise occurred in November 2019 on the R/V Alpha Crucis of the Oceanographic Institute of the University of São Paulo (IOUSP). We intended to discover and detail different geomorphological features, characterize free-living and symbiotic microorganisms, determine the chemosynthetic rates in relation to heterotrophic microbial production, and characterize the fauna and study their ecological and evolutionary links within and across ocean basins. All discoveries made during the cruise and their respective results will be presented separately in several papers that comprise this special volume.

https://doi.org/10.1590/2675-2824070.22077pygs

15.

COVID-19 in the Perioperative Period of Cardiovascular Surgery: the Brazilian Experience
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Gomes, Walter J.

; Rocco, Isadora

; Pimentel, Wallace S.

; Pinheiro, Aislan H. B.

; Souza, Paulo M. S.

; Costa, Luiz A. A.

; Teixeira, Marjory M. P.

; Ohashi, Leonardo P.

; Bublitz, Caroline

; Begot, Isis

; Moreira, Rita Simone L

; Hossne Jr, Nelson A.

; Vargas, Guilherme F.

; Branco, João Nelson R.

; Teles, Carlos A.

; Medeiros, Eduardo A. S.

; Sáfadi, Camila

; Rampinelli, Amândio

; Moratelli Neto, Leopoldo

; Rosado, Anderson Rosa

; Mesacasa, Franciele Kuhn

; Capriata, Ismael Escobar

; Segalote, Rodrigo Coelho

; Palmieri, Deborah Louize da Rocha Vianna

; Jardim, Amanda Cristina Mendes

; Vianna, Diego Sarty

; Coutinho, Joaquim Henrique de Souza Aguiar

; Jazbik, João Carlos

; Coutinho, Henrique Madureira da Rocha

; Kikuta, Gustavo

; Almeida, Zely Sant'Anna Marotti de

; Feguri, Gibran Roder

; Lima, Paulo Ruiz Lucio de

; Franco, Anna Carolina

; Borges, Danilo de Cerqueira

; Cruz, Felipe Ramos Honorato De La

; Croti, Ulisses Alexandre

; Borim, Bruna Cury

; Marchi, Carlos Henrique De

; Goraieb, Lilian

; Postigo, Karolyne Barroca Sanches

; Jucá, Fabiano Gonçalves

; Oliveira, Fátima Rosane de Almeida

; Souza, Rafael Bezerra de

; Zilli, Alexandre Cabral

; Mas, Raul Gaston Sanchez

; Bettiati Junior, Luiz Carlos

; Tranchesi, Ricardo

; Bertini Jr, Ayrton

; Franco, Leandro Vieira

; Fernandes, Priscila

; Oliveira, Fabiana

; Moraes Jr, Roberto

; Araújo, Thiago Cavalcanti Vila Nova de

; Braga, Otávio Penna

; Pedrosa Sobrinho, Antônio Cavalcanti

; Teixeira, Roberta Tavares Barreto

; Camboim, Irla Lavor Lucena

; Gomes, Eduardo Nascimento

; Reis, Pedro Horigushi

; Garcia, Luara Piovan

; Scorsioni, Nelson Henrique Goes

; Lago, Roberto

; Guizilini, Solange

Brazilian Journal of Cardiovascular Surgery

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Dez 2021, Volume 36 Nº 6 Páginas 725 - 735

Resumo: En | Texto: En | PDF: En

Abstract Introduction: We investigated the clinical course and outcomes of patients submitted to cardiovascular surgery in Brazil and who had developed symptoms/signs of coronavirus disease 2019 (COVID-19) in the perioperative period. Methods: A retrospective multicenter study including 104 patients who were allocated in three groups according to time of positive real time reverse transcriptase-polymerase chain reaction (RT-PCR) for the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2): group 1, patients who underwent cardiac surgery > 10 days after positive RT-PCR; group 2, patients with a positive RT-PCR within 10 days before or after surgery; group 3, patients who presented positive RT-PCR > 10 days after surgery. The primary outcome was mortality and secondary outcomes were postoperative complications, intensive care unit (ICU) length of stay, and postoperative days of hospitalization. Results: The three groups were similar with respect to age, the European System of Cardiac Operative Risk Evaluation score, and comorbidities, except hypertension. Postoperative complications and death were significantly higher in groups 2 and 3 than in group 1, and no significant difference between groups 2 and 3 was seen. Group 2 showed a high prevalence of surgery performed as an urgent procedure. Although no significant differences were observed in ICU length of stay, total postoperative hospitalization time was significantly higher in group 3 than in groups 1 and 2. Conclusion: COVID-19 affecting the postoperative period of patients who underwent cardiovascular surgery is associated with a higher rate of morbidity and mortality. Delaying procedures in RT-PCR-positive patients may help reduce risks of perioperative complications and death.

https://doi.org/10.21470/1678-9741-2021-0960

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