Pesquisa | SciELO

ABSTRACT Objectives: Currently, not much is known about the interactions between voice and growth hormone (GH). We have described large kindred with isolated GH deficiency (IGHD) due to a GHRH receptor mutation, resulting in severe short stature and high-pitched voice. These IGHD individuals have little interest in GH treatment, as they consider themselves “short long-lived people”, rather than patients. Interestingly, they report normal general quality of life, but they rate their Voice-Related Quality of Life (V-RQOL) as low. Here, we assessed the social and auditory-perceptual impacts of artistic-intervention voice therapy with semioccluded vocal tract exercises (SOVTE) and choral singing, on their voices. Material and methods: Seventeen GH-naïve adult IGHD individuals were enrolled in a single-arm interventional pre-post study with 13 weekly sessions of choir singing over 90 days. Outcome measures were V-RQOL scores, self-assessment of voice, and auditory-perceptual analysis (GRBAS scale, G: grade of the severity of dysphonia; R: roughness; B: breathiness; A: asthenia; and S: strain). Results: Marked improvements in total (p = 0.0001), physical (p = 0.0002), and socioemotional (p = 0.0001) V-RQOL scores and in self-assessment of voice (p = 0.004) were found. The general grades of vocal deviation (p = 0.0001), roughness (p = 0.0001), breathiness (p = 0.0001) and strain (p = 0.0001) exhibited accentuated reductions. Conclusions: Voice therapy with semioccluded vocal tract exercises and choral training improved social impact and perceptual voice assessments in IGHD subjects and markedly improved their voice-related quality of life. This is particularly important in a setting where GH replacement therapy is not widely accepted.

https://doi.org/10.20945/2359-3997000000449

Screening of Alkaloid-Producing Endophytic Penicillium Strains from Amazon Medicinal Plants by Electrospray Ionization Mass Spectrometry (ESI-MS) and Principal Component Analysis (PCA)
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Silva-Filho, Francinaldo A. da

; Souza, Marjory M. M. de

; Rezende, Gabriel O.

; Silva, Felipe M. A. da

; Cruz, Jeferson C. da

; Silva, Gilvan F. da

; Souza, Afonso D. L. de

; Souza, Antonia Q. L. de

Journal of the Brazilian Chemical Society

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Set 2021, Volume 32 Nº 9 Páginas 1832 - 1839

Resumo: En | Texto: En | PDF: En

The genus Penicillium is among the most promising alkaloid-producing fungal and therefore plays an important role in terms of producing molecules with biotechnological potential. Thus, in order to identify alkaloid-producing fungi, 25 endophytic Penicillium strains previously isolated from Amazon medicinal plants were subject to an integrative approach based on direct infusion positive electrospray ionization mass spectrometry (ESI-MS) and principal component analysis (PCA). The multivariate analysis pointed paxiline (1), glandicoline B (2), roquefortine C (3), and oxaline (5) as responsible for the segregation of three promising alkaloid-producing groups, been these groups constituted for P. chrysogenum, P. oxalicum, P. paxilli, and P. rubens strains. These alkaloids and the glandicoline A (4) were tentatively identified by multiple-stage mass spectrometry. In addition, compounds 1 and 2 were isolated and confirmed by using 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. Overall, the chemical profile analysis by ESI-MS along with PCA provided a simple and effective approach to screening alkaloid-producing Penicillium strains for biotechnological applications.

https://doi.org/10.21577/0103-5053.20210074 20 downloads

Multifunctionality of βCD/Ofloxacin and HPβCD/Ofloxacin Complexes: Improvement of the Antimicrobial Activity and Apoptosis Induction on Lung Adenocarcinoma A549 Cells
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Amaro, Bolivar R.

; Alves, Caio C. S.

; Ferreira, Gabriella F.

; Carvalho, Paloma E.

; Silva, Jeferson G. da

; Souza, Cleonice A.

; Cardoso Jr., Oswaldo

; Carli, Alessandra P.

; Machado, Fabiana S.

; Denadai, Ângelo M. L.

; Castro, Sandra B. R.

Journal of the Brazilian Chemical Society

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Dez 2020, Volume 31 Nº 12 Páginas 2628 - 2637

Resumo: En | Texto: En | PDF: En

The ofloxacin (OFLOX) is a second-generation synthetic antibiotic that can be classified as a multifunctional drug, but is a poorly soluble drug, which influences its efficiency. The inclusion complexes of OFLOX with β-cyclodextrin (βCD) or hydroxypropyl-β-cyclodextrin (HPβCD) can improve the chemical characteristics of the drug; however, studies showing the biological activity of these inclusion complexes are still scarce. The present work aimed to investigate the multifunctionality of the OFLOX and their inclusion complexes. Thus, the 1:1 βCD/OFLOX or HPβCD/OFLOX were prepared and analyzed by Fourier transform infrared spectroscopy (FTIR), thermogravimetric and differential thermal analysis (TGA and DTA), 1H nuclear magnetic resonance (NMR) and isothermal titration calorimetry (ITC). The antitumor and antibacterial effects were assessed. The results confirm the formation of the inclusion complexes, which had lower minimum inhibitory concentration (MIC) values, higher cytotoxicity and promoted the apoptosis. The present study showed, for the first time, the promising effects of the inclusion complexes as antitumor, improving the biological activities of the uncomplexed ofloxacin.

https://doi.org/10.21577/0103-5053.20200145 259 downloads

Gaseous exchanges of corn and weeds under competition and water regimes
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Freitas, Claudia D. M.

; Pamplona, Juliana de P.

; Lins, Hamurábi A.

; Souza, Matheus de F.

; Dombroski, Jeferson L. D.

; Silva, Daniel V.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Jul 2020, Volume 24 Nº 7 Páginas 465 - 473

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O déficit hídrico pode alterar funções fisiológicas vitais e afetar negativamente o crescimento das plantas. Esses efeitos podem ser intensificados pelas plantas daninhas que competem pela água disponível para as culturas. O objetivo foi avaliar os efeitos da competição e do déficit hídrico temporário do solo sobre a transpiração (E), condutância estomática (gs) e taxa fotossintética (A) do milho, da Urochloa decumbens e da Bidens pilosa L. O delineamento experimental foi em blocos casualizados, com oito repetições. Os tratamentos foram arranjados em esquema fatorial 5 x 2, com o primeiro fator correspondente às espécies milho + U. decumbens, milho + B. pilosa, e milho, U. decumbens e B. pilosa sem competição, e o segundo fator dois regimes hídricos, irrigação diária e restrição hídrica, que correspondeu à suspensão da irrigação desde o início do estágio V3 do milho até o momento em que seus estômatos ficaram totalmente fechados. As espécies B. pilosa e U. decumbens agravaram em diferentes intensidades os efeitos negativos sobre E, gs e A do milho em condição de restrição hídrica. A U. decumbens foi mais agressiva em relação a B. pilosa em competição com o milho, em ambos regimes hídricos. A competição com o milho afetou a E e gs da U. decumbens nos dois regimes hídricos, mas a A foi alterada sob déficit hídrico. Por outro lado, os componentes fisiológicos de B. pilosa não foram afetadas pela competição com o milho, nos dois regimes hídricos.

ABSTRACT Water deficits can alter vital physiological functions and negatively affect plant growth. These effects can be intensified by weeds competing for water available to crops. The objective of this study was to evaluate the effects of competition and temporary soil water deficit on the transpiration (E), stomatal conductance (gs) and photosynthetic rate (A) of corn, Urochloa decumbens, and Bidens pilosa L. The experimental design was in randomized blocks with eight repetitions. The treatments were arranged in a 5 x 2 factorial scheme, with the first factor corresponding to the species: corn + U. decumbens, corn + B. pilosa, and corn, U. decumbens, and B. pilosa without competition. The second factor was two water regimes: daily irrigation and water restriction, which corresponded to the suspension of irrigation from the beginning of the V3 stage of corn until the time when their stomata were completely closed. The species B. pilosa and U. decumbens worsened the negative effects on E, gs, and A of corn at different intensities under water restriction. The U. decumbens was more aggressive in relation to B. pilosa while coexisting with corn in both water regimes. The competition with corn affected the E and gs of U. decumbens in the two water regimes, but the A was altered under water deficit. On the other hand, the physiological components of B. pilosa were not affected by its coexistence with corn in the two water regimes.

https://doi.org/10.1590/1807-1929/agriambi.v24n7p465-473 433 downloads

Resistance training and hormone replacement increase MMP-2 activity, quality and quantity of bone in ovariectomized rats
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Souza, Markus V.C

; Lino, Anderson D. S

; Ruffoni, Leandro G. D

; Domingos, Mateus M.

; Barbosa, Marina R.

; Rodrigues, Maria F C.

; Ferreira, Fabiano C.

; Tomaz, Luciane M.

; Canevazzi, Gustavo H. R.

; Silva, Natália S.

; Teixeira, Jeferson A. A.

; Leite, Richard D.

; Shiguemoto, Gilberto E.

; Perez, Sérgio E. A.

Motriz: Revista de Educação Física

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2017, Volume 23 Nº 4 elocation e1017107

Resumo: En | Texto: En | PDF: En

Abstract AIMS The aim of the present study was to investigate the influence of resistance training (RT) and hormone replacement (HR) on MMP-2 activity, biomechanical and physical properties bone of ovariectomized (OVX) rats. METHODS Sprague-Dawley female rats were grouped into six experimental groups (n = 11 per group): sham-operated sedentary (SHAM Sed), ovariectomized sedentary (OVX Sed), sham-operated resistance training (SHAM RT), ovariectomized resistance training (OVX RT), ovariectomized sedentary hormone replacement (OVX Sed-HR), and ovariectomized resistance training hormone replacement (OVX RT-HR). HR groups received implanted silastic capsules with a 5% solution of 17β-estradiol (50 mg 17β-estradiol/ml of sunflower oil). In a 12-week RT period (27 sessions; 4-9 climbs) the animals climbed a 1.1 m vertical ladder with weights attached to their tails. Biomechanical and physical bone analyses were performed using a universal testing machine, and MMP-2 activity analysis was done by zymography. RESULTS Bone density and bone mineral content was higher in the RT and HR groups. The MMP-2 activity was higher in the RT and HR groups. The biomechanical analysis (stiffness, fracture load and maximum load) demonstrated better bone tissue quality in the RT associated with HR. CONCLUSION The RT alone as well as when it is associated with HR was efficient in increasing MMP-2 activity, biomechanical and biophysical properties bone of ovariectomized rats.

https://doi.org/10.1590/s1980-6574201700040003 751 downloads

Annularins I and J: New Metabolites Isolated from Endophytic Fungus Exserohilum rostratum
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Pinheiro, Eduardo A. A.

; Borges, Fabio C.

; Pina, Jeferson R. S.

; Ferreira, Leila R. S.

; Cordeiro, Jorgeffson S.

; Carvalho, Josiwander M.

; Feitosa, André O.

; Campos, Francinete R.

; Barison, Andersson

; Souza, Afonso D. L.

; Marinho, Patrícia S. B.

; Marinho, Andrey M. R.

Journal of the Brazilian Chemical Society

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Ago 2016, Volume 27 Nº 8 Páginas 1432 - 1436

Resumo: En | Texto: En | PDF: En

The paper describes the isolation of ergosterol peroxide, monocerin, annularin I and annularin J compounds of Exserohilum rostratum biomass extracts. Compounds were isolated by silica gel column chromatography in polarity gradient and the structures were determined by nuclear magnetic resonance (NMR) and mass spectrometry (MS) methods. Monocerin, annularin I and annularin J compounds were tested against Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis bacteria and showed moderate activity. Annularin I and annularin J compounds are new natural products.

https://doi.org/10.5935/0103-5053.20160140 931 downloads

Studies on thermal and viscoelastic properties of vinyl ester resin and its composites with glass fiber
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Garay, André C.

; Paese, Lucas T.

; Souza, Jeferson A.

; Amico, Sandro C.

Matéria (Rio de Janeiro)

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Mar 2015, Volume 20 Nº 1 Páginas 64 - 71

Resumo: En | Texto: En | PDF: En

Vinyl ester resins are widely used in sandwich composite structures. Because of their good resistance to chemicals, flexibility and easy processing, these sandwich are extensively applied in the marine sector. These composites are typically manufactured by liquid molding processes, especially infusion. In this study, RTM light was used to inject the polymeric resin into the mold cavity, flowing in the space between the impermeable core and the mold walls, where the fibrous medium was. In this process, viscosity, gel time and curing time of the resin are very important parameters. This work addressed the curing and post-curing characteristics of a vinyl ester resin, and also the characteristics of neat and reinforced vinyl ester using dynamic mechanical analysis (DMA). The increase in shear rate did not significantly influence resin viscosity within the studied range. Differential scanning calorimetry showed the efficiency of the post-curing stage, with the decrease in residual enthalpy. With DMA, it was possible to determine gel time and gel temperature, which yielded similar values to those found by the SPI (Society of the Plastics Industry) method, indicating that the simpler SPI method can be reliably used for that.

https://doi.org/10.1590/S1517-707620150001.0009 3950 downloads

Mating disruption with low density diffusers for the management of oriental fruit moths (Lepidoptera: Tortricidae) in apple orchards under subtropical climate in southern Brazil
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Monteiro, Lino Bittencourt

; Souza, Alexander

; Argenton, Jeferson

Revista Brasileira de Fruticultura

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Dez 2013, Volume 35 Nº 4 Páginas 1007 - 1016

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Mariposa-oriental, Grapholita molesta Busk, e moscas-das-frutas, Anastrepha fraterculus L., são importantes pragas de macieira em clima Subtropical no sul do Brasil e o controle é normalmente realizado com inseticidas. Uma estratégia alternativa para o controle de G. molesta é confusão sexual, por meio de feromônios. Estratégias de confusão sexual, usando uma baixa densidade de dispersores (20) por hectare, foram testados em comparação com pesticidas convencionais para o controle de G. molesta em pomares comerciais de maçã Gala em Fraiburgo, SC, por um período de cinco anos. O período médio de eficiência em campo da formulação na interrupção de acasalamento, durante cinco anos, foi de 113 dias. Neste período, o índice de interrupção de acasalamento em parcelas de confusão sexual foi de 84,8 %. Danos à Gala por larvas de mariposa-oriental foi baixa (<0,1%) em parcelas de confusão sexual, mas não diferiram das parcelas convencionais, exceto no terceiro ano. O uso da formulação de confusão sexual permitiu uma redução média de 5,2 tratamentos com inseticidas por ano em pomares de Gala durante o período de eficiência em campo. Foi necessário aplicar 1,0 e 1,2 aplicações de inseticidas para o controle de G. molesta e A. fraterculus, respectivamente. Confusão sexual com uma baixa densidade de difusores provou ser uma alternativa eficaz aos métodos convencionais para o controle de G. molesta em pomares de maçã Gala em clima Subtropical no sul do Brasil .

The oriental fruit moth, Grapholita molesta Busck, and fruit flies, Anastrepha fraterculus L., are the important apple pests under Subtropical climate in Southern Brazil, and control is normally accomplished with insecticides. An alternative strategy for the control of G. molesta is mating disruption, through the use of pheromones. Mating disruption strategies using a low density of dispensers (20) per hectare were tested in comparison with conventional pesticides for control of G. molesta in commercial Gala apple orchards in Fraiburgo, SC, for a period of five years. The average field efficiency period of mating disruption formulation over five years was 113 days. In this period the mating interruption index on mating disruption plots was 84.8% over five years. Damage to Gala apples by oriental moth larvae was low (<0.1%) in mating disruption plots but did not differ from conventional plots, except in the third year. The use of mating disruption allowed for an average reduction of 5.2 insecticide treatments per year in Gala orchards during field efficiency period. It was necessary to apply 1.0 and 1.2 applications of insecticide to control of G. molesta and A. fraterculus, respectively. Mating disruption with a low density of diffusers proved to be an effective alternative to conventional methods for control of G. molesta in Gala apple orchards in subtropical climate in southern Brazil.

1832 downloads Citado 2 vezes em SciELO

Three-dimensional numerical modeling of RTM and LRTM processes
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Isoldi, Liércio A.

; Oliveira, Cristiano P.

; Rocha, Luiz A. O.

; Souza, Jeferson A.

; Amico, Sandro C.

Journal of the Brazilian Society of Mechanical Sciences and Engineering

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Jun 2012, Volume 34 Nº 2 Páginas 105 - 111

Resumo: En | Texto: En | PDF: En

Resin Transfer Molding (RTM) is a manufacturing process in which a liquid resin is injected into a closed mold pre-loaded with a porous fibrous preform, producing complex composite parts with good surface finishing. Resin flow is a critical step in the process. In this work, the numerical study of the resin flow in RTM applications was performed employing a general Computational Fluid Dynamics software which does not have a specific RTM module, making it necessary to use the Volume of Fluid method for the filling problem solution. Examples were presented and compared with analytical, experimental and numerical results showing the validity and effectiveness of the present study, with maximum difference among these solutions of around 8%. Besides, based on the computational model for the RTM process, a new computational methodology was developed to simulate Light Resin Transfer Molding (LRTM). In this process, resin is injected into the mold through an empty injection channel (without porous medium) which runs all around the perimeter of the mold. The ability of FLUENT® package to simulate geometries which combine porous media regions with open (empty) regions was used. Two specific cases were simulated, showing the differences in time and behavior between RTM and LRTM processes.

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10.

Viabilidade celular de nanofibras de polímeros biodegradáveis e seus nanocompósitos com argila montmorilonita
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Goes, Alfredo M.

; Carvalho, Sandhra

; Oréfice, Rodrigo L.

; Avérous, Luc

; Custódio, Tassiana A.

; Pimenta, Jeferson G.

; Souza, Matheus de B.

; Branciforti, Marcia C.

; Bretas, Rosario E. S.

Polímeros

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2012, Volume 22 Nº 1 Páginas 34 - 41

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Mantas não tecidas de nanofibras de três polímeros biodegradáveis poli(ácido láctico), PDLLA, poli(Ε-caprolactona), PCL, e poli(butileno adipato-co-tereftalato), PBAT e seus nanocompósitos com uma nanoargila montmorilonita (MMT) foram produzidas por eletrofiação. A morfologia, o comportamento térmico e a estrutura interna das nanofibras foram analisados por microscopia eletrônica de varredura e transmissão, calorimetria diferencial de varredura e difração de raios X, respectivamente. Observou-se que as nanofibras dos nanocompósitos possuíam diâmetros menores do que os correspondentes polímeros puros e que as nanofibras de PBAT puro e de PBAT/MMT apresentavam a menor cristalinidade de todas as mantas. A viabilidade celular de todas as nanofibras foi analisada pela técnica de redução do sal de tetrazolium pelo complexo enzimático piruvato desidrogenase presente na matriz de mitocôndrias (teste MTT). Os resultados mostraram que nenhuma manta nanofibrílica apresentou toxicidade às células e que as nanofibras de PBAT puro e seu nanocompósito propiciaram ainda um ambiente mais favorável ao desenvolvimento celular de fibroblastos de cardiomiócitos do que as condições oferecidas pelo controles, provavelmente por apresentarem menores diâmetros e baixa cristalinidade em relação às demais nanofibras. Estes resultados mostram o potencial de uso destas mantas nanofibrílicas como suportes de crescimento celular.

Non-woven mats of nanofibers of three biodegradable polymers, viz. poly(lactic acid), PDLLA, poly(Ε-caprolactone), PCL, and poly(butylene adipate-co-terephthalate), PBAT, and their nanocomposites with montmorillonite nanoclay (MMT) were produced by electrospinning. The morphology, thermal behavior and internal structure of the nanofibers were analyzed by scanning and transmission electron microscopy, differential scanning calorimetry and wide angle X-ray diffraction, respectively. The nanofibers of the nanocomposites had lower diameters than the nanofibers of the corresponding neat polymers, while the nanofibers from PBAT and PBAT/MMT were the least crystalline. The cell viability of all the nanofibers was analyzed by reduction of the tetrazolium salt by the pyruvate dehydrogenase enzymatic complex present in the mitochondria (MTT test). None of the nanofibers was toxic to the cells and the PBAT and PBAT/MMT nanofibers exhibited a more favorable environment for developing fibroblasts from cardiomyocytes than the control, probably due to their low crystallinity. These results demonstrated the potential use of nanofibers´ mats as scaffolds for cell growth.

6428 downloads

11.

Efeito da época do ano e período do dia sobre os parâmetros fisiológicos de reprodutores caprinos no semi-árido paraibano
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Silva, Gustavo de A.

; Souza, Bonifácio B. de

; Alfaro, Carlos E. P.

; Silva, Elisângela M. N. da

; Azevedo, Solange A.

; Azevedo Neto, Jéferson

; Silva, Rosangela M. N. da

Revista Brasileira de Engenharia Agrícola e Ambiental

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Dez 2006, Volume 10 Nº 4 Páginas 903 - 909

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Objetivou-se, com este trabalho, verificar o efeito da época do ano e período do dia sobre os parâmetros fisiológicos de reprodutores caprinos mestiços de Anglo-Nubiana com caprinos sem padrão racial definido, no Semi-árido paraibano. Utilizaram-se 20 caprinos machos, distribuídos em um delineamento inteiramente casualizado no esquema fatorial 2 x 2, duas épocas: E1, maio a agosto e E2, setembro a dezembro, e dois turnos, manhã e tarde, repetidos no tempo, totalizando 80 parcelas. Para os parâmetros hematológicos estudou-se apenas o efeito da época do ano. Os parâmetros fisiológicos, temperatura retal e freqüência respiratória, foram influenciados (P < 0,01) pelo turno, enquanto a temperatura superficial sofreu influência (P < 0,01) de ambos os fatores, turno do dia e época do ano. Os parâmetros hematológicos, hemácias, hematócrito e volume globular médio, foram influenciados (P < 0,05) pela época do ano. A pouca variação dos parâmetros fisiológicos de caprinos mestiços de Anglo-Nubiana com sem padrão racial definido, demonstra alto grau de adaptação à temperaturas elevadas. No período de maior temperatura a perda de calor através da forma evaporativa, respiração, determina a elevação do hematócrito e do volume globular médio.

The objective of this work was to verify the effect of time of year and period of day on physiological parameters of reproductive Anglo-Nubiana crossbred goats with goats without a defined racial pattern race, in the semi-arid Paraiba. 20 male goats were used, allocated into a completely randomized design in a 2 x 2 factorial scheme, two periods: E1, May to August, and E2, September to December, and two periods, morning and afternoon, repeated in time, totaling 80 plots. For the hematological parameters only the effect of time of year was studied. The physiological parameters, rectal temperature and respiratory frequency were influenced (P < 0.01) by the period, while the surface temperature (ST) suffered influence (P < 0.01) of both factors, day period and time of year. The parameters hematological, erythrocyte (HE), hematocrit (HT) and medium globular volume (MGV) were influenced (P < 0.05) by the time of the year. The little variation of the physiological parameters of Anglo-Nubiana crossbred goats without defined racial patlern demonstrates a high adaptation degree to high temperatures. During the period of higher temperature, the heat loss in the form of evaporation and respiration determines the elevation of the hematócrit and medium globular volume.

3639 downloads

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ti:dengue and publication_year:2015 and aff_country:Brasil

Veja abaixo a lista completa de índices de pesquisa que podem ser usados:

Cód. do Índice	Elemento
ti	título do artigo
au	autor
kw	palavras-chave do artigo
subject	assunto (palavras do título, resumo e palavras-chave)
ab	resumo
ta	título abreviado da revista (ex. Cad. Saúde Pública)
journal_title	título completo da revista (ex. Cadernos de Saúde Pública)
la	código do idioma da publicação (ex. pt - Português, es - Espanhol)
type	tipo do documento
pid	identificador da publicação
publication_year	ano de publicação do artigo
sponsor	financiador
aff_country	código do país de afiliação do autor
aff_institution	instituição de afiliação do autor
volume	volume do artigo
issue	número do artigo
elocation	elocation
doi	número DOI
issn	ISSN da revista
in	código da coleção SciELO (ex. scl - Brasil, col - Colômbia)
use_license	código da licença de uso do artigo

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