Investigou-se a atividade antimicrobiana da lama termal (Complejo Termal Copahue, Argentina) sobre microorganismos da microbiota autóctone do homem, agentes infecciosos da comunidade, hospitalares e cepas AT CC. As cepas corresponderam a cocos Gram positivos, bacilos Gram negativos, leveduras e cepas AT CC. A lama foi obtida da Laguna Sulfurosa. A atividade inibitória se demonstrou utilizando fase líquida (FL) mediante a prova de difusão em ágar e por estudos de cinética bactericida. Com a primeira se observou halo inibitório perante Staphylococcus aureus e Cándida albicans, não se visualizando inibição para o resto dos microorganismos. O estudo dinâmico através do tempo sobre S. aureus, Enterococcus faecalis, Staphylococcus epidermis e C. albicans demonstrou ação inibitória antes das 6 hs de incubação, enquanto não se observou inibição perante os bacilos Gram negativos. É o primeiro trabalho que demonstra a atividade antimicrobiana da FL sobre as cepas ensaiadas.

The antimicrobial activity of thermal mud from Copahue Thermal Complex against microorganisms from human microbiota, community and hospital infections agents and AT CC strains was investigated. Thermal mud samples were obtained from Sulfurous Lagoon located within the Complex. To determine the antimicrobial properties of the liquid phase (LP) of Copahue thermal mud we used in vitro assays, agar diffusion test and bactericidal rate, using gram-positive cocci, gram-negative bacilli, yeast and AT CC strains. Inhibition zones around the disk against Staphylococcus aureus and Candida albicans was showed with the first technique. No inhibition zones were observed for the rest of the microorganisms assayed. With the killing curve technique, inhibitory action before 6 h of incubation was observed against S. aureus, E. faecalis, S. epidermidis and C. albicans while there was no inhibition against gram negative bacillus. This is the first study that demonstrates the antimicrobial activity of LP on certain assayed strains.

Se investigó la actividad antimicrobiana del fango termal (Complejo Termal Copahue, Argentina) sobre microorganismos de la microbiota autóctona del hombre, agentes infecciosos de la comunidad, hospitalarios y cepas AT CC. Las cepas correspondieron a cocos Gram positivos, bacilos Gram negativos, levaduras y cepas AT CC. El fango se obtuvo de la Laguna Sulfurosa. La actividad inhibitoria se demostró utilizando fase líquida (FL) mediante la prueba de difusión en agar y por estudios de cinética bactericida. Con la primera se observó halo inhibitorio frente a Staphylococcus aureus y Cándida albicans, no visualizándose inhibición para el resto de los microorganismos. El estudio dinámico a través del tiempo sobre S. aureus, Enterococcus faecalis, Staphylococcus epidermis y C. albicans demostró acción inhibitoria antes de las 6 hs de incubación, mientras que no se observó inhibición frente a los bacilos Gram negativos. Es el primer trabajo que demuestra la actividad antimicrobiana de la FL sobre las cepas ensayadas.

The aim of the present paper was to evaluate the larvicidal effect of Enterococcus faecalis CECT7121 (Ef7121) on the Toxocara canis cycle both in vitro and in vivo. For the in vitro experiments, T. canis larvae were incubated with the supernatants of Ef7121 (EI) and mutant Ef7121 (EIm), in a pre-culture of Ef7121 (EII) and in a fresh culture with Ef7121 (EIII) and the Ef7121 mutant strain (EIIIm). The viability of the larvae was calculated after a 48 h incubation. A significant reduction of the viability of T. canis larvae was observed in EI, EII and EIII. A decrease of this inhibitory effect was observed in EIm and EIIIm (p = 0.008). In the in vivo experiments, mice were orally inoculated with three doses of Ef7121. To study the probiotic persistence in the intestine, the animals were sacrificed every four days and their intestines were dissected. The initial average bacterial levels were 9.7 x 10(4) for Ef7121 (colony forming units/g). At the end of the assay the levels were 1.46 x 10(4). No bacterial translocation was detected in mesenteric lymphatic nodules and spleen. Ef7121 interference with the biological cycle was evaluated in mice challenged with T. canis. The interference was significant when the mice were challenged with probiotic and T. canis simultaneously (p = 0.001), but it was not significant when the challenge was performed 15 days after administration of the bacterial inoculum (p = 0.06). In conclusion, Ef7121 possessed in vitro and in vivo larvicidal activity.