Abstract Purpose To assess the feasibility of thoracoscopic transdiaphragmatic approach for biopsy of all lung lobes and to determine the optimal intercostal space (ICS) for biopsy of each lung lobe. Methods Ten rabbits were positioned in dorsal recumbency. Total thoracoscopy lung biopsy was made combined transdiaphragmatic approach and right ICS approaches. A camera port was made in the transdiaphragmatic approach and the instrument port was made of ICS 7 and ICS 9. A pre tied loop ligature was placed to performed a caudal lung lobe biopsy and to simulate biopsies of the others lung lobes. Results Biopsy of the cranial aspect of the right caudal lung lobe was performed at ICS 9. Simulated biopsy of the accessory lung lobe was performed at ICS 9. Simulated lung biopsy of the right cranial and middle lung lobes was performed at ICS 7. The caudal and dorsal aspect of the right caudal lung lobe was not visualized by telescope at transdiaphragmatic approach, and biopsy was not performed. Conclusions Thoracoscopic transdiaphragmatic approach for lung lobes biopsies was a feasible technique, except for the caudal aspect of the right caudal lung lobe. An ideal intercostal port for biopsy of each right lung lobe was determined.

RESUMO A qualidade de mudas de espécies arbóreas é de fundamental importância para se obter êxito em projeto de reflorestamento ou de exploração comercial. Este trabalho foi desenvolvido com o objetivo de avaliar a influência de dois ambientes (AMB-1, casa de vegetação; AMB-2, câmara plástica instalada dentro da casa de vegetação) e concentrações de nutrientes aplicados via fertirrigação (0, 50, 100 e 150% de uma solução nutritiva padrão) na produção de mudas de Tabebuia aurea, utilizando delineamento inteiramente casualizado, com quatro repetições. Foram avaliadas as seguintes características: altura (H), diâmetro do coleto (DC), número de folhas (NF), área foliar (AF), massa seca da parte aérea (MSPA), massa seca de raiz (MSR) e massa seca total (MST), relação H/DC, área foliar específica (AFE), razão de área foliar (ARF) e índice de qualidade de Dickson (IQD). A análise dos dados revelou que houve interação significativa entre os fatores para a maioria das variáveis. O uso da câmara plástica (AMB-2) proporcionou maior crescimento das mudas de Tabebuia aurea. Soluções de fertirrigação com concentração de nutrientes variando de 80 a 100% da solução padrão proporcionam mudas de Tabebuia aurea de melhor qualidade.

ABSTRACT The quality of seedlings of tree species is crucial to succeed in reforestation project or commercial exploitation. This work was to evaluate the influence of two environments (AMB-1, greenhouse; AMB-2, plastic camera installed inside the greenhouse) and concentrations of nutrients applied by fertigation (0; 50; 100 and 150% of a patterns nutrient solution) in the production of seedlings of Tabebuia aurea using design was completely randomized, with four replications. The following growth characteristics were evaluated: height, stem diameter, number of leaves, leaf area, dry matter aerial part, dry root matter, dry root weight, total dry matter, height/stem diameter ratio, specific leaf area, leaf area ratio and Dickson quality index (IQD). Data analysis revealed that there was significant interaction between the factors for most variables. The use of the plastic chamber (AMB-2) provided greater growth of Tabebuia aurea seedlings. Fertigation solutions with nutrient concentrations varying from 80 to 100% of the standard solution provide better quality Tabebuia aurea seedlings.

OBJETIVO: Avaliar a técnica mais promissora para a coleta de células tronco adultas de medula óssea de coelhos para a utilização do mesmo como modelo experimental na medicina regenerativa. MÉTODOS: Foram utilizados 30 coelhos divididos em 2 grupos: GRUPO A, n=8, onde realizamos a coleta de sangue de medula óssea (MO) da crista ilíaca e grupo B, n=22, onde realizamos a coleta de sangue da medula óssea da epífise do fêmur. Após as coletas, realizamos a separação das células mononucleadas através do gradiente de densidade (Ficoll-Hystopaque). Através da câmara de Neubauer realizamos a contagem das células mononucleadas por ml. Testamos a viabilidade celular através do método Tripan Blue. RESULTADOS: Na coleta de sangue de MO na crista ilíaca obtivemos a média de 1 ml durante 1 hora de procedimento cirúrgico, obtendo a quantidade de 3,6 .10(6) células/ml, enquanto que a punção na epífise do fêmur obtivemos a média de 3 ml durante 30 minutos de procedimento cirúrgico obtendo a quantidade de 11,79.10(6) cél./ml diminuindo o óbito dos animais. CONCLUSÃO: A análise para a obtenção de maior número de células mononucleadas/ml de sangue de medula óssea de coelho foi mais satisfatória na região da epífise do fêmur em comparação com a crista ilíaca.

PURPOSE: To assess the technique for the collection of rabbit bone marrow stem cells from different regions to be used as an experimental model in regenerative medicine. METHODS: Thirty rabbits were allocated into 2 groups: GROUP A, n=8, animals that underwent bone marrow blood (BMB) harvesting from the iliac crest; and GROUP B: including 22 rabbits that underwent BMB harvesting from the femur epiphysis. After harvesting, mononuclear cells were isolated by density gradient centrifugation (Ficoll - Histopaque). The number of mononuclear cells per ml was counted in a Neubauer chamber and cell viability was checked through Tripan Blue method. RESULTS: Harvesting from the iliac crest yielded an average of 1 ml of BMB and 3,6.10(6) cells/ml over 1 hour of surgery, whereas an average of 3ml of BMB and 11,79.10(6) cells./ml were obtained in 30 min from the femur epiphysis with a reduced animal death rate. CONCLUSION: The analysis for the obtention of a larger number of mononuclear cells/ml from rabbit bone marrow blood was more satisfactory in the femur epiphysis than in the iliac crest.