Siloxane-poly(methyl methacrylate) (PMMA) hybrid was synthesized based on the sol-gel method and applied to Ti-6Al-4V through dip-coating. The structural feature of the hybrid was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TG); its morphological properties were analyzed through scanning electron microscope (SEM), energy dispersive spectrometry (EDS), atomic force microscopy (AFM) and contact angle. The electrochemical behavior and corrosion resistance of both the hybrid coating and the uncoated substrate were investigated based on electrochemical impedance spectroscopy (EIS) and scanning vibrating electrode technique (SVET). Results showed homogeneous coating formation due to the rigid hybrid network formed during polymerization, hydrolysis and condensation reactions. This outcome suggested interaction between the organic (methyl methacrylate (MMA)) and inorganic (tetraethyl orthosilicate (TEOS)) phases, which was favored by the coupling agent (3-(trimethoxysilyl)propyl methacrylate (TMSM)). The S0M (PMMA-SiO2 hybrid Ti-6Al-4V coated alloy) film presented regular topography and adherence to the substrate. Electrochemical simulated body fluid (SBF) tests showed that the hybrid coating presented better barrier properties (168 h of the immersion time) than the uncoated substrate, fact that makes it an interesting material for anti-corrosive applications in biomaterials.

Formigas cortadoras de folhas são consideradas pragas para a agricultura devido à grande quantidade de material vegetal utilizado por elas para cultivar um fungo simbionte que lhes serve de alimento e enzimas. O mutualismo entre o fungo e as formigas é um ponto a ser explorado quando se considera sua possível aplicação em métodos alternativos para o controle desses insetos. Sabendo-se que algumas plantas são naturalmente resistentes aos insetos fitófagos, alguns produtos naturais (metabólitos secundários) devem ser avaliados em relação às suas propriedades inseticidas e/ou fungicidas. Neste trabalho foram isoladas oito cumarinas de quatro espécies de plantas e o efeito no desenvolvimento do fungo simbionte das formigas cortadeiras Atta sexdens foi determinado. Com exceção da clausarina, todas as outras cumarinas foram inibitórias de 64 µg mL-1 à 80 µg mL-1 sendo que a xantiletina inibiu o fungo na concentração de 25 µg mL-1.

Leaf-cutting ants are known to be a serious pest for agriculture due to the high amounts of vegetal matter from crops used by them in order to cultivate a symbiotic fungus on which they rely for food and enzymes. The mutualism between the fungus and the ants is a point to be explored when alternative methods of control are being thought of. Considering that some plants are naturally resistant to phytophagous insects, some natural products (secondary metabolites) should be evaluated with respect to their insecticide and/or fungicide properties. In this paper we isolated eight coumarins from four different plant species and we determined their effect on the development of the symbiotic fungus of the leaf-cutting ant Atta sexdens. With the exception of clausarin, all the other coumarins were inhibitory from 64 µg mL-1 through 80 µg mL-1 and xanthyletin inhibited the fungus at 25 µg mL-1

A incomparabilidade sempre foi uma limitação entre os estudos sobre atividade física, em função da utilização de vários instrumentos e pontos de corte inconsistentes. Comparou-se o nível de atividade física de adultos do Estado de São Paulo e da cidade de Pelotas, Rio Grande do Sul, no Brasil. Ambos os centros usaram delineamento transversal de base populacional, com amostragem em múltiplos estágios. A prática de atividade física foi avaliada com a versão curta do Questionário Internacional de Atividade Física. A prevalência de sedentarismo foi três vezes maior em Pelotas do que em São Paulo. Já a proporção de pessoas muito ativas foi significativamente maior em Pelotas. A prevalência de atividade física insuficiente (sedentários + irregularmente ativos) foi similar em ambos os centros. O nível sócio-econômico associou-se inversamente com o nível de atividade física nos dois centros. Entre as pessoas insuficientemente ativas, as de São Paulo estão ao menos praticando uma pequena quantidade de atividade física. Já entre os suficientemente ativos, as pessoas de Pelotas têm maior nível de atividade física.

Lack of comparability has been a major limitation in studies on physical activity, due to the utilization of different methodological instruments and inconsistent cut-off points. This study aims to compare the levels of physical activity in adults from two Brazilian areas: (a) São Paulo, the richest State in the country; (b) Pelotas, a medium-sized southern Brazilian city. Both sites used cross-sectional population-based designs, with multiple-stage sampling strategies. Level of physical activity was assessed with the short version of the International Physical Activity Questionnaire. Prevalence of sedentary lifestyle was three times higher in Pelotas than in São Paulo. On the other hand, the proportion of very active subjects was significantly higher in Pelotas. The proportion of insufficiently active individuals (sedentary + irregularly active) was almost identical between the sites. Socioeconomic status was inversely related to level of physical activity in both sites. Among the insufficiently active subjects, those living in São Paulo are at least engaged in a limited amount of activity. On the other hand, among sufficiently active people, those living in Pelotas are more active.

A incomparabilidade sempre foi uma limitação entre os estudos sobre atividade física, em função da utilização de vários instrumentos e pontos de corte inconsistentes. Comparou-se o nível de atividade física de adultos do Estado de São Paulo e da cidade de Pelotas, Rio Grande do Sul, no Brasil. Ambos os centros usaram delineamento transversal de base populacional, com amostragem em múltiplos estágios. A prática de atividade física foi avaliada com a versão curta do Questionário Internacional de Atividade Física. A prevalência de sedentarismo foi três vezes maior em Pelotas do que em São Paulo. Já a proporção de pessoas muito ativas foi significativamente maior em Pelotas. A prevalência de atividade física insuficiente (sedentários + irregularmente ativos) foi similar em ambos os centros. O nível sócio-econômico associou-se inversamente com o nível de atividade física nos dois centros. Entre as pessoas insuficientemente ativas, as de São Paulo estão ao menos praticando uma pequena quantidade de atividade física. Já entre os suficientemente ativos, as pessoas de Pelotas têm maior nível de atividade física.

Lack of comparability has been a major limitation in studies on physical activity, due to the utilization of different methodological instruments and inconsistent cut-off points. This study aims to compare the levels of physical activity in adults from two Brazilian areas: (a) São Paulo, the richest State in the country; (b) Pelotas, a medium-sized southern Brazilian city. Both sites used cross-sectional population-based designs, with multiple-stage sampling strategies. Level of physical activity was assessed with the short version of the International Physical Activity Questionnaire. Prevalence of sedentary lifestyle was three times higher in Pelotas than in São Paulo. On the other hand, the proportion of very active subjects was significantly higher in Pelotas. The proportion of insufficiently active individuals (sedentary + irregularly active) was almost identical between the sites. Socioeconomic status was inversely related to level of physical activity in both sites. Among the insufficiently active subjects, those living in São Paulo are at least engaged in a limited amount of activity. On the other hand, among sufficiently active people, those living in Pelotas are more active.

OBJECTIVES: To describe the prevalence, frequency, type, motivation for, and demographic and socioeconomic distribution of leisure-time physical activity (LTPA) among adults in Brazil. METHODS: The data source for our study was the Brazilian Living Standards Measurement Survey (LSMS) (Pesquisa sobre Padrões de Vida), which was conducted in 1996 and 1997. This survey studied a multistage stratified probabilistic sample of 4 893 households, which included 11 033 persons who were 20 years of age or older. The surveyed households were selected in the two most populous Brazilian regions, the Northeast and the Southeast, where in total 70% of all Brazilians live. The selected persons responded to a series of questions concerning their leisure-time physical activities. RESULTS: Only 13% of the Brazilians surveyed reported performing at least a minimum of 30 minutes of LTPA on one or more days of the week, and only 3.3% reported doing the recommended minimum of 30 minutes on 5 or more days of the week. In younger age groups, men were more active than were women. However, this difference sharply decreased with increasing age, and by the age range of 40 to 45 years the prevalence of LTPA was similarly low in both genders. Men reported engaging in more team sports, and women reported more walking/jogging activities, but walking/jogging was relatively more common in both genders when physical activity was performed on 5 or more days of the week. Recreation was by far the leading reason given by men to engage in LTPA, while recreation, health concerns, and even esthetic concerns were all relevant for women. In both genders, health concerns tended to be relatively more important for those exercising more days of the week. Also in both genders, increasing age was associated with more frequent LTPA, more walking/jogging than team sports, and more health concerns reasons than reasons related to recreation. Among both men and women there was a strong association between LTPA and socioeconomic status, measured either by income or schooling, independent of age, region, and urban or rural place of residence. CONCLUSIONS: The prevalence of adult LTPA in Brazil was much lower than the levels that have been reported for developed countries. However, the demographic and social distribution of LTPA in Brazil followed a pattern similar to the one usually observed in developed nations, where men tend to be more active than women, increasing age limits LTPA, and higher socioeconomic status is associated with more LTPA. Our data will provide a baseline to evaluate the impact on LTPA of "Agita Brasil" ("Move, Brazil"), an initiative to encourage physical activity that was implemented in the country after 1997.

OBJETIVOS: Describir la prevalencia, frecuencia, tipo, motivación y distribución demográfica y socioeconómica de la actividad física en horas libres (AFHL) entre adultos en el Brasil. MÉTODOS: La fuente de datos para nuestro estudio fue la Encuesta para la Medición del Nivel de Vida (EMNV) en el Brasil, que se llevó a cabo en 1996 y 1997. Mediante esta encuesta se estudió una muestra probabilística multietápica y estratificada de 4 893 hogares que abarcó a 11 033 personas de 20 años de edad o mayores. Los hogares encuestados se seleccionaron en la dos regiones más pobladas del Brasil, el Noroeste y el Sureste, donde viven 70% de todos los habitantes del Brasil. Las personas seleccionadas contestaron una serie de preguntas acerca de sus actividades físicas en horas libres. RESULTADOS: Sólo 13% de los brasileños encuestados indicaron que hacían un mínimo de 30 minutos de AFHL un día de la semana o más, y únicamente 3,3% indicó que lo hacía como mínimo 30 minutos durante 5 días de la semana o más, que es la cantidad recomendada. Entre las personas más jóvenes se encontró que los hombres eran más activos que las mujeres. No obstante, esta diferencia descendió notablemente a medida que aumentó la edad, de tal manera que en el grupo de 40 a 45 años la prevalencia de AFHL era igualmente baja en ambos sexos. Los hombres indicaron que participaban en deportes en equipo, mientras que las mujeres dijeron que participaban en actividades tales como caminar o trotar (jogging), pero estas últimas fueron las actividades más comunes en ambos sexos en aquellos casos en que la actividad física se practicaba 5 días a la semana o más. El recreo fue, con mucho, la razón que los hombres citaron con mayor frecuencia para explicar por qué practicaban AFHL, mientras que el recreo, la salud y hasta razones estéticas fueron citados como aspectos importantes por las mujeres. En ambos sexos la salud fue un motivo más importante entre las personas que practicaban alguna actividad física durante un mayor número de días a la semana. También se observó en ambos sexos un aumento de la frecuencia de AFHL conforme aumentaba la edad, con una mayor tendencia a caminar o a trotar que a practicar deportes y con una mayor preocupación por la salud que por el recreo. Tanto entre los hombres como entre las mujeres se observó una fuerte asociación entre la AFHL y el estado socioeconómico, medido ya sea en términos de ingresos o de escolaridad, independientemente de la edad, la religión, o la residencia en una zona urbana o rural. CONCLUSIONES: La prevalencia de AFHL en la población adulta del Brasil fue mucho más baja que la notificada en países en desarrollo. Sin embargo, la distribución demográfica y social de la AFHL en el Brasil tuvo un patrón similar al observado habitualmente en países desarrollados, donde los hombres tienden a ser más activos que las mujeres, la mayor edad limita la AFHL, y un mayor nivel socioeconómico se asocia con más AFHL. Nuestros datos servirán de base para evaluar los efectos de la AFHL de "Agita Brasil" ("Muévete, Brasil"), iniciativa en pro de la actividad física que se puso en marcha en el país después de 1997.

Seeds Citrus oils (C. sinensis, C. limon and C. reticulata) extraction with hexane in a soxhlet apparatus and through supercritical fluid (CO2) were done. Besides triglycerides, the oils obtained with hexane comprised volatile compounds such as terpenes and fatty alcohols, esters, and aldehydes. However, the oils obtained by extraction with supercritical fluid presented only triglycerides. These results indicate that the extraction using supercritical fluid presents better selectivity. The activity of the oils on the development of the ant symbiotic fungus, Leucoagaricus gongylophorus, showed week activity and the topic insecticide assay showed better activity for the tangerine seed oil.

A investigação fitoquímica dos extratos de caules e folhas da espécie endêmica Raulinoa echinata Cowan, Rutaceae, levou ao isolamento de alcalóides furoquinolínicos (esquimianina, kokusaginina, maculina e flindersiamina) e quinolônicos (1-metil-2-n-nonil-4-quinolona, 2-n-nonil-4-quinolona e 2-fenil-1-metil-4-quinolona). Os alcalóides isolados mostraram atividade antifúngica contra Leucoagaricus gongylophorus, o fungo simbionte de formigas cortadeiras (Atta spp). Quando testados in vitro contra formas tripomastigotas de Trypanosoma cruzi, os alcalóides foram inativos ou parcialmente ativos. Neste trabalho descrevem-se o isolamento dos alcalóides, a elucidação estrutural dos alcalóides quinolônicos e a estrutura obtida por difração de raio-X da flindersiamina, juntamente com os resultados dos bioensaios realizados.

Phytochemical survey of stems and leaves extracts of the South Brazilian endemic plant Raulinoa echinata Cowan, Rutaceae, led to the isolation of known furoquinoline alkaloids: the widespread skimmianine; kokusaginine, maculine, flindersiamine, and also quinolone derivatives: 1-methyl-2-n-nonyl-4-quinolone, 2-n-nonyl-4-quinolone and 1-methyl-2-phenyl-4-quinolone. These alkaloids showed antifungal activity against Leucoagaricus gongylophorus; the symbiotic fungus of leaf-cutting ants (Atta sexdens). They were inactive or displayed weak inhibitory activity when assayed in vitro against trypomastigote forms of Trypanosoma cruzi. In this paper, the isolation, structure elucidation and bioactivity results of these compounds are reported together with the X-ray structure of flindersiamine.

Da casca do caule de Picramnia teapensis Tul. foram isolados dois novos benzoatos triterpênicos, o 3alfa,7beta-dibenzoato do ácido lup-20(29)-en-28-óico e o 7beta-benzoato do ácido 3alfa-hidroxi-lup-20(29)-en-28-óico. A elucidação estrutural desses compostos foi fundamentada na análise dos dados espectroscópicos. Outros compostos conhecidos, beta-sitosterol, estigmasterol, lupeol e epilupeol, foram identificados em mistura por CG-EM. Nenhum dos triterpenos benzoilados mostrou efeito inibitório no crescimento in-vitro do Leucoagaricus gongylophorus (Fisher), também citado como Leucocoprinus gongylophorus (Heim), syn Rozites gongylophora (Möller), o fungo simbionte cultivado pelas formigas cortadeiras de folhas Atta sexdens L.

Two new benzoic acid esters of triterpene alcohols [lup-20(29)-en-28-oic acid 3alpha,7beta-dibenzoate and 3alpha-hydroxy-lup-20(29)-en-28-oic acid 7beta-benzoate] were isolated from the stem bark of Picramnia teapensis Tul. The structures of these compounds were established on the basis of spectral analyses. Other known compounds, beta-sitosterol, estigmasterol, lupeol and epilupeol, were identified in mixture by GC-MS. The triterpene esters have not shown in-vitro inhibitory effect on the growth of Leucoagaricus gongylophorus (Fisher), referred also as Leucocoprinus gongylophorus (Heim), syn Rozites gongylophora (Möller), the symbiotic fungus cultivated by the leaf-cutting ant Atta sexdens L.

O fungo simbionte Leucoagaricus gongylophorus (Möller) Singer, sin. de Rozites gongylophora Möller, cultivado pela formiga cortadeira Atta sexdens L. teve seu desenvolvimento fortemente inibido por frações obtidas das folhas de gergelim, Sesamum indicum L. (Pedaliaceae). Total inibição foi observada na concentração de 2,5 mg/ml, sendo verificada inibição de 50% com algumas frações na concentração de 1,25 mg/ml. A análise cromatográfica do extrato hexânico revelou a ocorrência de uma mistura de ácidos graxos, onde os componentes majoritários foram os ácidos tetradecanóico, hexadecanóico, octadecanóico, icosanóico, docosanóico e 9,12,15 octadecatrienóico. A separação desses compostos durante as várias etapas do fracionamento resultou em perda ou diminuição da atividade inibitória sobre o fungo, indicando que a inibição observada pode ser consequência da ação conjunta de alguns dos compostos presentes nas folhas e não de uma única substância.

The symbiotic fungus Leucoagaricus gongylophorus (Möller) Singer (syn. Rozites gongylophora Möller) cultivated by the leaf-cutting ant Atta sexdens L. had its development strongly inhibited by crude extracts obtained from leaves of sesame, Sesamum indicum L. (Pedaliaceae). With most of the fractions from these extracts total inhibition was observed at 2.5 mg/ml, whereas inhibition of 50% was observed with some fractions at 1.25 mg/ml. Chromatographic analysis of the hexane extract showed that it was composed by a mixture of fatty acids, of which tetradecanoic, hexadecanoic, octadecanoic, icosanoic, docosanoic and 9,12,15 octadecatrienoic acids were the major components. The separation of these compounds during the different steps of purification resulted in no or lower inhibitory effect for most of these fractions, indicating that the inhibitory activity observed could be due to the joint action of these compounds present in the leaf tissue, rather than to the action of a single substance.