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The association between body mass index and varicocele: A meta-analysis
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Xiao-Bin, Guo

; Fang-Lei, Wu

; Hui, Xia

; Cheng, Yang

; Zhi-Xuan, Cai

; Zhi-Peng, Huang

; Cun-Dong, Liu

; Wen-Bin, Guo

International braz j urol

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Fev 2021, Volume 47 Nº 1 Páginas 8 - 19

Resumo: En | Texto: En | PDF: En

ABSTRACT Objective: Recently, several studies have found that obesity had a protective effect against varicocele, but no meta-analysis has confirmed this finding. Therefore, we conducted this meta-analysis to investigate the association between body mass index (BMI) and varicocele. Material and Methods: We searched for studies in PubMed, Science Direct and the Cochrane Library from inception until February 2018. The association between BMI and varicocele was assessed by pooling the odds ratios (ORs). Results: Eleven eligible studies with a total study population of 1.376.658 participants were included in our analysis. According to BMI, the subjects were defined as belonging to the obese, overweight and underweight groups. Our results showed that the obese group had a lower risk of varicocele when compared with the normal weight group (odds ratio [OR] 0.46, 95% confidence intervals [CIs] 0.37-0.58). Additionally, an overweight BMI had a protective effect against varicocele (OR 0.70, 95% CIs, 0.56-0.86). However, underweight patients had a more than 30% higher risk of varicocele (OR 1.31, 95% CI, 1.04-1.64). Furthermore, there was no publication bias in any of the analyses. Conclusions: Our study demonstrates that BMI is negatively associated with the presence of varicocele.

https://doi.org/10.1590/s1677-5538.ibju.2019.0210 588 downloads

Effects of Heat Stress on Expression of Heat Shock Proteins in the Small Intestine of Wenchang Chicks
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Yu, ZQ

; Tian, JY

; Wen, J

; Chen, Z

Brazilian Journal of Poultry Science

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2021, Volume 23 Nº 3 elocation eRBCA-2020-1430

Resumo: En | Texto: En | PDF: En

ABSTRACT In this study, immunohistochemistry and real-time fluorescent-based quantitative PCR were used to evaluate the expression of heat shock protein (HSP) 60, HSP70, and HSP90 in the small intestine of Wenchang chicks. Compared with the control group (CK), the positive expression of HSP60 and HSP60 mRNA in the heat stress group (HS) were initially higher and subsequently lower in the duodenum (p<0.01). In the HS jejunum, the levels of HSP60 were higher (p<0.01) and the HSP60 mRNA was lower (p<0.05). Whereas the levels of HSP60 in the HS ileum were higher (p<0.05) and then lower (p<0.01), and the HSP60 mRNA was higher (p<0.01). The levels of HSP70 were higher (p<0.01) and the HSP70 mRNA was lower in the duodenum (p<0.05), while the expression of both HSP70 and HSP70 mRNA was higher in the jejunum (p<0.01) and the ileum (p<0.05) of the HS. In the HS duodenum the levels of HSP90 were lower and then higher (p<0.01), and the HSP90 mRNA was higher (p<0.01). The expression of both HSP90 and HSP90 mRNA was higher in the HS jejunum (p<0.05). The levels of HSP90 were lower while the HSP90 mRNA was higher in the HS ileum (p<0.01). These results indicate that heat stress disturbed the expression of HSP60, HSP70, and HSP90 in the small intestine of chicks.

https://doi.org/10.1590/1806-9061-2020-1430 44 downloads

Kidney displaced by giant retroperitoneal liposarcoma in HIV patient
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Wen, Sheng-Chen

; Lin, Chunhsuan

International braz j urol

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Ago 2020, Volume 46 Nº 4 Páginas 673 - 675

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1590/s1677-5538.ibju.2019.0515 257 downloads

Diminutive Incision Acromioplasty Assisted with Arthroscopy in the Treatment of Chinese Patients with Subacromial Impingement Syndrome
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Wen, Z-Q

; Pan, J

; Chen, Z

; Du, J-Y

; Gu, P-C

; Lin, X-J

West Indian Medical Journal

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Jun 2019, Volume 68 Nº 2 Páginas 165 - 170

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT Background: Many causes can lead to shoulder pain and subacromial impingement syndrome (SIS) is the most frequently recorded disorders. The aim of this study was to evaluate the clinical effects of diminutive incision acromioplasty assisted with arthroscopy for the treatment of Chinese patients with subacromial impingement syndrome. Subject and Methods: Twenty-two patients with 24-painful shoulders subacromial impingement syndrome were enrolled. All painful shoulders were in Grades II (8) and III (16) according to Neer's classification. Detailed physical examination was performed. Conventional radiography and subsequent magnetic resonance imaging (MRI) of the shoulder region of all patients were done. The University of California at Los Angeles Shoulder (UCLA) score system was used for all patients to evaluate their satisfaction after surgery. The preoperative recordings of the UCLA scores were collected and all enrolled cases including 24-painful shoulders were available for follow-up in 1, 3, 6, 12 months after surgery. Results: According to the UCLA scoring system, the symptom of all painful shoulders were improved after one year postoperatively. The average score before surgery from 15.4 points increased to 31.2 points postoperatively, showing a statistical difference (p < 0.05). Conclusions: A diminutive incision acromioplasty assisted with arthroscopy is a reliable approach to treat Chinese patients with subacromial impingement syndrome. All painful shoulders were obviously improved in one year after surgery.

ABSTRACT Antecedentes: Muchas causas pueden provocar dolor de hombro y síndrome de compresión subacromial (SIS) es el trastorno más frecuentemente registrado. El objetivo de este estudio fue evaluar la clínica. Efectos de la acromioplastia con incisión diminuta asistida con artroscopia para el tratamiento de Pacientes chinos con síndrome de pinzamiento subacromial. Sujeto y métodos: Se incluyeron veintidós pacientes con síndrome de afectación subacromial de 24-hombros dolorosos. Todos los hombros dolorosos estaban en Grados II (8) y III (16) de acuerdo con la clasificación de Neer. Se realizó examen físico detallado. Se realizaron radiografías convencionales y, posteriormente, imágenes de resonancia magnética (IRM) de la región del hombro de todos los pacientes. El sistema de puntuación de la Universidad de California en Los Angeles Shoulder (UCLA) se utilizó para que todos los pacientes evaluaran su satisfacción después de la cirugía. Los registros preoperatorios de las puntuaciones de UCLA se recopilaron y todos los casos incluidos, incluidos 24-hombros dolorosos, estaban disponibles para el seguimiento en 1, 3, 6 y 12 meses después de la cirugía. Resultados: De acuerdo con el sistema de puntuación de UCLA, el síntoma de todos los hombros dolorosos mejoró después de un año después de la operación. La puntuación promedio antes de la cirugía de 15.4 puntos aumentó a 31.2 puntos después de la operación, mostrando una diferencia estadística (p < 0.05) Conclusiones: Una acromioplastia de incisión diminuta asistida con artroscopia es un enfoque confiable para tratar a pacientes chinos con síndrome de pinzamiento subacromial. Todas las lesiones dolorosas se mejoraron obviamente en un año después de la cirugía.

https://doi.org/10.7727/wimj.2014.308

Balloon dilation for failed pyeloplasty in children?
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Duan, Haifeng

; Zhu, Wei

; Zhong, Wen

; Li, Xiaohang

; Zeng, Guohua

International braz j urol

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Jun 2019, Volume 45 Nº 3 Páginas 617 - 620

Resumo: En | Texto: En | PDF: En

ABSTRACT Objective: Pyeloplasty is considered the gold standard treatment for ureteropelvic junction obstruction (UPJO). However, the failure rate of pyeloplasty is as high as 10% and repeat pyeloplasty is more difficult. This study aimed to evaluate the efficacy of balloon dilatation for failed pyeloplasty in children. Materials and Methods: Between 2011 and 2017, 15 patients, aged 6 months to 14 years, were treated with balloon dilation for restenosis of UPJO after a failed pyeloplasty. Ultrasound and intravenous urography were used to evaluate the primary outcome. Success was defined as the relief of symptoms and improvement of hydronephrosis, which was identified by ultrasound at the last follow-up. Results: All patients successfully completed the operation, 13 patients by retrograde approach and 2 patients by antegrade approach. Thirteen patients were followed for a median of 15 (4 to 57) months and 2 patients were lost to follow-up. Resolution of the hydronephrosis was observed in 5 cases. The anteroposterior diameter (APD) of the pelvis decreased by an average of 12.4 ± 14.4mm. Eight patients needed another surgery. The average postoperative hospital stay was 1.78 ± 1.4 days. Two patients experienced fever after balloon dilation. No other complications were found. Conclusions: Balloon dilatation surgery is safe for children, but it is not recommended for failed pyeloplasty in that group of patients, owing to the low success rate.

https://doi.org/10.1590/s1677-5538.ibju.2018.0407 679 downloads

Prognostic significance of Body Mass index in patients with localized renal cell carcinoma
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Wang, Chengtao

; Chen, Zebin

; Dong, Jun

; Wen, Bixiu

; Fang, Yong

International braz j urol

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Dez 2018, Volume 44 Nº 6 Páginas 1147 - 1155

Resumo: En | Texto: En | PDF: En

ABSTRACT Objective: To investigate the relationship between the pretreatment body mass index (BMI) and the clinical outcomes in patients with localized stage I - III renal cell carcinoma (RCC) surgically treated. Materials and Methods: From January 2000 to December 2012, 798 patients with stage I - III RCC were recruited from First Affiliated Hospital and Cancer Center of Sun Yat - Sen University. Patients were divided into two groups of BMI < 25 kg / m2 or BMI ≥ 25 kg / m2 according to the World Health Organization classifications for Asian populations. The differences in the long-term survival of these two BMI groups were analyzed. Results: The 5 - year failure - free survival rates for BMI < 25 kg / m2 and BMI ≥ 25 kg / m2 groups were 81.3% and 93.3%, respectively (P = 0.002), and the 5 - year overall survival rates were 82.5% and 93.8%, respectively (P = 0.003). BMI was a favored prognostic factor of overall survival and failure - free survival in a Cox regression model. Conclusions: Pretreatment body mass index was an independent prognostic factor for Chinese patients surgically treated, localized stage I - III RCC.

https://doi.org/10.1590/s1677-5538.ibju.2017.0629 466 downloads

Re: Gaseous bladder tamponade secondary to emphysematous cystitis
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Chen, Yu-Chen

; Chen, Hao-Wei

; Juan, Yung-Shun

; Wu, Wen-Jeng

; Tsai, Chia-Chun

International braz j urol

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Jun 2018, Volume 44 Nº 3 Páginas 653 - 654

Resumo: En | Texto: En | PDF: En

https://doi.org/10.1590/s1677-5538.ibju.2017.0701 747 downloads

Thermal Properties and Kinetics of Al/α-MnO2 Nanostructure Thermite
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Song, Jia-Xing

; Fang, Xiang

; Guo, Tao

; Bei, Feng-Li

; Ding, Wen

; Zhang, Xiao-Nan

; Yao, Miao

; Yu, Hong-Jun

Journal of the Brazilian Chemical Society

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Fev 2018, Volume 29 Nº 2 Páginas 404 - 411

Resumo: En | Texto: En | PDF: En

In this work, thermal properties and kinetics of Al-nanoparticles/α-MnO2 nanorods thermite were reported. The α-MnO2 nanorods were synthesized using a hydrothermal method and were characterized by X-ray powder diffraction (XRD) and X-ray photoelectron spectra (XPS), then combined with Al nanoparticles based on the ultrasonic mixing method to prepare the nanostructure thermite. Besides, both pure components and mixture were characterized by field emission scanning electron microscopy (FE-SEM) to observe their morphologies and structures. Subsequently, the thermal properties of Al/α-MnO2 nanostructure thermite were studied on the basis of thermogravimetric-differential scanning calorimetry (TG-DSC). According to the TG-DSC tests, the calculation results of activation energy for kinetics of Al/α-MnO2 thermite were obtained using different isoconversional methods. It was found that Al/α-MnO2 nanostructure thermite has high heat release and low onset temperature, and the heat release of the nanostructure thermite was approximately 1146.6 J g -1.

https://doi.org/10.21577/0103-5053.20170154 966 downloads

Emergency percutaneous nephrostomy versus emergency percutaneous nephrolithotomy in patients with sepsis associated with large uretero-pelvic junction stone impaction: a randomized controlled trial
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Hsu, Chi-Sen

; Wang, Chung-Jing

; Chang, Chien-Hsing

; Tsai, Po-Chao

; Chen, Hung-Wen

; Su, Yi-Chun

International braz j urol

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Jun 2017, Volume 43 Nº 3 Páginas 481 - 488

Resumo: En | Texto: En | PDF: En

ABSTRACT Introduction A randomized trial was conducted prospectively to evaluate the efficacy, related complications, and convalescence of emergency percutaneous nephrolithotomy compared to percutaneous nephrostomy for decompression of the collecting system in cases of sepsis associated with large uretero-pelvic junction stone impaction. Materials and Methods The inclusion criteria included a WBC count of 10.000/mm3 or more and/or a temperature of 38°C or higher. Besides, all enrolled patients should maintain stable hemodynamic status and proper organ perfusions. A total of 113 patients with large, obstructive uretero-pelvic junction stones and clinical signs of sepsis completed the study protocol. Of those, 56 patients were placed in the emergency percutaneous nephrostomy group, while the other 57 patients were part of the percutaneous nephrolithotomy group. The primary end point was the time until normalization of white blood cells (WBC) at a count of 10.000/mm3 or less, and a temperature of 37.4°C or lower. The secondary end points included the comparison of analgesic consumption, length of stay, and related complications. Statistical analysis was performed using SPSS® version 14.0.1. The Mann-Whitney U test, chi-square test, and Fisher’s exact test were used as appropriate. Results The length of hospital stays (in days) was 10.09±3.43 for the emergency percutaneous nephrostomy group and 8.18±2.72 for the percutaneous nephrolithotomy group. This set of data noted a significant difference between groups. There was no difference between groups in regard to white blood cell count (in mm3), time to normalization of white blood cell count (in days), body temperature (in ºC), time to normalization of body temperature (in days), C-reactive proteins (in mg/dL), time taken for C-reactive proteins to decrease over 25% (in days), procalcitonin (in ng/mL), or complication rates. Conclusions This study confirms that emergency percutaneous nephrolithotomy may be as safe as early percutaneous nephrolithotomy in a selected low risk patients with sepsis-associated large, obstructive stone.

https://doi.org/10.1590/s1677-5538.ibju.2015.0643 1286 downloads

10.

The Expression of Can and Camk is Associated with Lipogenesis in the Muscle of Chicken
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Yang, Y

; Song, J

; Fu, R

; Sun, Y

; Wen, J

Brazilian Journal of Poultry Science

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Set 2015, Volume 17 Nº 3 Páginas 287 - 292

Resumo: En | Texto: En | PDF: En

ABSTRACTIntramuscular fat (IMF) content in chickens significantly contributes to meat quality. The main objective of this study was to assess the expression of calcineurin (CaN) and Ca2+/calmodulin-dependent protein kinase (CaMK) in lipogenesis in chicken muscle. Chickens were slaughtered and sampled at 4, 8, and 16 weeks of age. IMF content and the expression of CaN subunits and CaMK isoforms were measured in the thigh muscle tissue. The results showed that the IMF contents were greater at 16 weeks compared with those at 4 and 8 weeks (p<0.05). Transcription of fatty acid synthase (FAS) and fatty acid translocase CD36 (FAT/CD36) mRNA significantly increased with age, from four to 16 weeks (p<0.05). The mRNA levels of CaNB and CaMK IV were significantly lower at 16 weeks than at four weeks (p<0.05), but CaMK II mRNA levels were significantly higher than at four weeks (p<0.05). In order to evaluate the role of CaMK and CaN in adipogenesis, SV cells were incubated in standard adipogenic medium for 24 h and treated with specific inhibitor of CaMK and CaN. The expressions of CCAAT/enhancer binding protein b (C/EBPb, sterol regulatory element-binding protein 1 (SREBP1),and peroxisome proliferation-activated receptor g (PPARγ)were dramatically enhanced by the CsA, CaN inhibitor (p<0.05). KN93, CaMK II inhibitor, dramatically repressed the expression of those lipogenic gene (p<0.05). These results indicated that CaN and CaMK had different effects on adipogenesis in the muscle of chickens.

https://doi.org/10.1590/1516-635X1703287-292 1186 downloads

11.

Utilization and perioperative complications of laparoscopic cryoablation vs. robotic partial nephrectomy for localized renal tumors
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Weinberg, Aaron C.

; Woldu, Solomon L.

; Wen, Timothy

; Deibert, Christopher M.

; Korets, Ruslan

; Badani, Ketan K.

International braz j urol

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Jun 2015, Volume 41 Nº 3 Páginas 473 - 485

Resumo: En | Texto: En | PDF: En

<title>ABSTRACT</title><sec><title>Objective:</title>To compare the utilization, perioperative complications and predictors of LCA versus RPN in the treatment of localized renal tumors.</sec><sec><title>Methods:</title>From the Nationwide Inpatient Sample we identified patients undergoing RPN or LCA for the treatment of localized renal tumors from October 2008 through 2010. Patient and hospital-specific factors which predict postoperative complications and use of LCA were investigated.</sec><sec><title>Results:</title>14,275 patients with localized renal tumors were identified: 70.3% had RPN and 29.7% had LCA. LCA was more common in older patient and at hospitals without robotic consoles. No difference was identified in perioperative complications (0.2% vs. 0.2%), transfusion (5.1% vs. 6.2%), length of stay (2.9 vs. 3.0 days) or median cost ($41,753 vs. $44,618) between the groups, LCA vs. RPN. On multivariate analysis sicker patients were more likely to have LCA (OR 1.34, p=0.048) and sicker patients had greater postoperative complications (OR 3.30, p<0.001); LCA did not predict more complications (OR 1.63, p=0.138) and LCA was performed at hospitals without RCs (OR 0.02, p<0.001). Limitations include observational study design, inability to assess disease severity, operative time, or body mass index, which may affect patient selection and outcomes.</sec><sec><title>Conclusions:</title>More patients had RPN vs. LCA; surgical technique was not predictive of postoperative complications. As technology develops to treat localized renal tumors, it will be important to continue to track outcomes and costs for procedures including RPN and LCA.</sec>

https://doi.org/10.1590/S1677-5538.IBJU.2014.0294 1133 downloads

12.

Assessment of survival of patients with metastatic clear cell renal cell carcinoma after radical cytoreductive nephrectomy versus no surgery: a SEER analysis
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Xiao, Wen-Jun

; Zhu, Yao

; Dai, Bo

; Zhang, Hai-Liang

; Ye, Ding-Wei

International braz j urol

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Abr 2015, Volume 41 Nº 2 Páginas 288 - 295

Resumo: En | Texto: En | PDF: En

Purposes To examine the factors related to the choice of cytoreductive nephrectomy (CN) for patients with metastatic clear cell renal cell carcinoma (mCCRCC), and compare the population-based survival rates of patients treated with or without surgery in the modern targeted therapy era. Materials and Methods From 2006 to 2009, patients with mCCRCC were identified from SEER database. The factors that affected patients to be submitted to CN were examined and propensity scores for each patient were calculated. Then patients were matched based upon propensity scores. Univariable and multivariable cox regression models were used to compare survival rates of patients treated with or without surgery. Finally, sensitivity analysis for the cox model on a hazard ratio scale was performed. Results Age, race, tumor size, T stage and N stage were associated with nephrectomy univariablely. After the match based upon propensity scores, the 1-, 2-, and 3-year cancer-specific survival rate estimates were 45.1%, 27.9%, and 21.7% for the no-surgery group vs 70.6%, 52.2%, and 41.7% for the surgery group, respectively (hazard ratio 0.42, 95%CI: 0.35-0.52, log-rank P<0.001). In multivariable Cox proportional hazard regression model, race, T stage, N stage and median household income were significantly associated with survival. Sensitivity analysis on a hazard ratio scale indicated that the hazard ratio might be above 1.00 only when the unknown factor had an opposite effect on survival which was 3-fold than CN. Conclusion The results of our study showed that CN significantly improves the survival of patients with metastatic CCRCC even in the targeted therapy era.

https://doi.org/10.1590/S1677-5538.IBJU.2015.02.15 1556 downloads

13.

LOS GRANDES GRUPOS EMPRESARIALES CHINOS: ENTENDIENDO LOS MECANISMOS DEL CAPITALISMO DE ESTADO EN CHINA
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Lin, Li-Wen

; Milhaupt, Curtís J

Revista chilena de derecho

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Set 2013, Volume 40 Nº 3 Páginas 801 - 858

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

China now has the second largest number of Global Fortune 500 Companies in the world. Most of these are state-owned enterprises (SOEs) organized into massive corporate groups with a central government agency known as SASAC as their ultimate controlling shareholder. Despite their importance to China's domestic economy and outward investment strategy, many features of the SOE sector -particularly the organizational structure and governance characteristics of the SOE groups- remain a black box. Unpacking the black box requires moving away from the standard focus on agency costs in listed firms that predominates in the corporate governance literature. Instead, we examine the relational ecology in which the SOE groups exist, with a focus on institutionalized mechanisms linking the business groups with other organs of the party-state. We argue that through these linkages, Chinese managerial elites in the economy have assembled what Mancur Olson called an "encompassing organization" -a coalition whose members own so much of society that they have important incentives to be actively concerned about how productive it is. EXPOSING the mechanisms of Chinese state capitalism in this way raises many questions for scholars and policymakers, the salience of which increases as the global interaction of Chinese firms expands: For example, is the rise of Chinese SOEs adequately explained by prevailing theories in the comparative corporate governance literature? How might the increased operation of Chinese SOEs in foreign markets change the institutional trajectory of corporate capitalism in China? Do the institutions of market and investment regulation in the U.S. adequately contemplate hybrid business-political actors like Chinese SOEs? By examining the organizational structure of SOEs, the Article provides a foundation for future research on a pivotal aspect of China's contemporary political economy.

China ahora es el segundo país que más empresas Global Fortune 500 tiene en el mundo. Casi todas ellas son empresas estatales (SOEs, acrónimo en inglés) organizadas dentro de grandes grupos empresariales, con un organismo central gubernamental denominado SASAC como accionista controlador final. A pesar de la importancia de estos grupos para la economía doméstica china y su estrategia de invertir en el exterior, muchas de las particularidades del sector SOE -especialmente su estructura organizacional y las características de gobierno corporativo de los distintos grupos empresariales- permanecen en la penumbra. Para salir de esa oscuridad se requiere dejar el análisis habitual basado en los costos de agencia de las empresas que cotizan en bolsa, predominante en la bibliografía sobre gobierno corporativo. Por el contrario, examinamos la ecología relacional en la que se mueven los grupos SOE, con atención en los mecanismos institucionalizados que unen los grupos empresariales con otros órganos del Estado-partido. Pensamos que a través de estos enlaces, las élites administradoras de la economía china han ensamblado lo que Mancur Olson llamó una "coalición englobante total" -una organización cuyos miembros deben tanto a la sociedad, lo que hace que tengan grandes incentivos para estar muy pendientes de la productividad del grupo SOE. Exponiendo de esta manera los mecanismos del capitalismo de Estado chino surgen muchas interrogantes para los investigadores y para los responsables de políticas públicas, las que se incrementan a medida que se expande la interacción global de las empresas chinas. Por ejemplo, ¿se explica adecuadamente el ascenso de las SOEs chinas mediante las teorías ordinarias de la bibliografía comparada de gobierno corporativo? ¿Cómo podría transformar la trayectoria institucional del capitalismo corporativo en China la cada vez mayor operación de las SOEs en el extranjero? ¿Las instituciones regulatorias del mercado y de las inversiones en Estados Unidos consideran adecuadamente a actores híbridos de negocios y política como son las SOEs chinas? Examinando la estructura organizativa de las SOEs, este artículo entrega los cimientos para futuras investigaciones sobre un aspecto esencial de la política económica contemporánea de China.

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14.

I Diretriz de Ressuscitação Cardiopulmonar e Cuidados Cardiovasculares de Emergência da Sociedade Brasileira de Cardiologia
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Gonzalez, MM

; Timerman, S

; Gianotto-Oliveira, R

; Polastri, TF

; Canesin, MF

; Schimidt, A

; Siqueira, AW

; Pispico, A

; Longo, A

; Pieri, A

; Reis, A

; Tanaka, ACS

; Santos, AM

; Quilici, AP

; Ribeiro, ACL

; Barreto, ACP

; Pazin-Filho, A

; Timerman, A

; Machado, CA

; Franchin Neto, C

; Miranda, CH

; Medeiros, CR

; Malaque, CMS

; Bernoche, C

; Gonçalves, DM

; Sant'Ana, DG

; Osawa, EA

; Peixoto, E

; Arfelli, E

; Evaristo, EF

; Azeka, E

; Gomes, EP

; Wen, FH

; Ferreira, FG

; Lima, FG

; Mattos, FR

; Galas, FG

; Marques, FRB

; Tarasoutchi, F

; Mancuso, FJN

; Freitas, GR

; Feitosa-Filho, GS

; Barbosa, GC

; Giovanini, GR

; Miotto, HC

; Guimarães, HP

; Andrade, JP

; Oliveira-Filho, J

; Fernandes, JG

; Moraes Junior, JBMX

; Carvalho, JJF

; Ramires, JAF

; Cavalini, JF

; Teles, JMM

; Lopes, JL

; Lopes, LNGD

; Piegas, LS

; Hajjar, LA

; Brunório, L

; Dallan, LAP

; Cardoso, LF

; Rabelo, MMN

; Almeida, MFB

; Souza, MFS

; Favarato, MH

; Pavão, MLRC

; Shimoda, MS

; Oliveira Junior, MT

; Miura, N

; Filgueiras Filho, NM

; Pontes-Neto, OM

; Pinheiro, PAPC

; Farsky, OS

; Lopes, RD

; Silva, RCG

; Kalil Filho, R

; Gonçalves, RM

; Gagliardi, RJ

; Guinsburg, R

; Lisak, S

; Araújo, S

; Martins, SCO

; Lage, SG

; Franchi, SM

; Shimoda, T

; Accorsi, TD

; Barral, TCN

; Machado, TAO

; Scudeler, TL

; Lima, VC

; Guimarães, VA

; Sallai, VS

; Xavier, WS

; Nazima, W

; Sako, YK

Arquivos Brasileiros de Cardiologia

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Ago 2013, Volume 101 Nº 2 Páginas 1 - 221

Resumo: Pt | Texto: Pt | PDF: Pt

https://doi.org/10.5935/abc.2013S006 554620 downloads

15.

Differentially expressed genes in a flock of Chinese local-breed chickens infected with a subgroup J avian leukosis virus using suppression subtractive hybridization
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Zhao, Guiping

; Zheng, Maiqing

; Chen, Jilan

; Wen, Jie

; Wu, Chunmei

; Li, Wenjuan

; Liu, Libo

; Zhang, Yuan

Genetics and Molecular Biology

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2010, Volume 33 Nº 1 Páginas 44 - 50

Resumo: En | Texto: En | PDF: En

Avian leukosis virus subgroup J (ALV-J) is a new type of virus that mainly induces myeloid leukosis (ML) in chickens. To further elucidate the pathogenesis of ALV-J infection and tumor development, expression profiles from the bone marrow tissue of 15 infected and 18 non-infected birds from a local-breed poultry-farm under naturally infected conditions, were analyzed by suppression-subtractive hybridization. The birds were diagnosed as ML+ (or ML-) by specific ALV-J detection methods, involving serological tests for antigens and antibodies, and RT-PCR to detect viral RNA. A total of 59 partial gene sequences were revealed by differential screening of 496 forward and 384 reverse subtracted cDNA clones. Of these, 22 identified genes, including 8 up-regulated and 14 down-regulated, were related to immune functions, these genes being, MHC B-G antigen, translationally-controlled tumor protein (TPT1/TPTC), transferrin and ferritin, hemoglobin and Carbonic anhydrase. Four of the down-regulated genes were selected for further analysis, in view of their predicted roles in infection and immunity by real-time qRT-PCR, using RNA collected from the same birds as those used for SSH. The four genes were expressed at significantly lower levels (p < 0.001) in ALV-J infected birds than in non-infected ones.

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