Two snapshot surveys to establish the diversity and ecological preferences of mosquitoes (Diptera: Culicidae) in the terra firme primary rain forest surrounding the Tiputini Biodiversity Station in the UNESCO Yasuní Biosphere Reserve of eastern Amazonian Ecuador were carried out in November 1998 and May 1999. The mosquito fauna of this region is poorly known; the focus of this study was to obtain high quality link-reared specimens that could be used to unequivocally confirm species level diversity through integrated systematic study of all life stages and DNA sequences. A total of 2,284 specimens were preserved; 1,671 specimens were link-reared with associated immature exuviae, all but 108 of which are slide mounted. This study identified 68 unique taxa belonging to 17 genera and 27 subgenera. Of these, 12 are new to science and 37 comprise new country records. DNA barcodes [658-bp of the mtDNA cytochrome c oxidase ( COI ) I gene] are presented for 58 individuals representing 20 species and nine genera. DNA barcoding proved useful in uncovering and confirming new species and we advocate an integrated systematics approach to biodiversity studies in future. Associated bionomics of all species collected are discussed. An updated systematic checklist of the mosquitoes of Ecuador (n = 179) is presented for the first time in 60 years.

Revisão do Grupo Leucosphyrus de Anopheles (Cellia) (Diptera, Culicidae). Apresenta-se a revisão taxonômica de 20 espécies do Grupo Leucosphyrus da Série Neomyzomyia de Anopheles (Cellia). Apresentam-se as descrições morfológicas dos adultos, machos e fêmeas, genitália masculina, pupa e larva de quarto estádio, bem como dados de bionomia, distribuição e discussão sistemática para cada espécie, incluindo caracteres diagnósticos. Foram acrescentadas chaves dicotômicas de identificação para as espécies, utilizando caracteres dos adultos fêmeas e larvas de quarto estádio. Quando possível, foram incluídos dados sobre a importância médica de cada espécie. Foram também apresentadas ilustrações dos adultos, das larvas de quarto estádio e das pupas. Os mapas de distribuição das espécies foram elaborados com base no material examinado, no entanto, quando possível utilizou-se dados adicionais publicados na literatura. Fornecem-se tabelas com as variações dos caracteres dos adultos, das cerdas das larvas de quarto estádio das pupas. São designados os neótipos de An. takasagoensis Morishita e An. sulawesi Koesoemawinangoen, e o lectótipo de An. balabacensis Baisas. O nome do autor de An. sulawesi previamente citado como sendo Waktoedi foi corrigido para Koesoemawinangoen.

Revision of the Leucosphyrus Group of Anopheles (Cellia) (Diptera, Culicidae). This is a comprehensive revision of 20 species of the Leucosphyrus Group of the Neomyzomyia Series of Anopheles (Cellia). Morphological description of the adults, male and female, male genitalia, pupa and fourth-instar larva are provided for each taxon in addition to bionomics, distribution data and systematic discussion for each species, including diagnostic characters. Identification keys for females and fourth-instar larvae are provided. When possible medical importance of each species is included. Illustrations of of the adults, fourth-instar larvae and pupae are provided. Distribution maps for each species are mainly based on the material examined; however, when possible published data were also used. Tables on adult character variations, fourth-instar larval and pupal setal branching are included as appendices. A neotype for An. takasagoensis Morishita and An. sulawesi Koesoemawinangoen, and a lectotype for An. balabacensis Baisas, are designated. The authorship of An. sulawesi previously cited as Waktoedi is corrected to Koesoemawinangoen.