Abstract Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility. In goats four ureaplasma isolates were obtained from vulvovaginal mucus, but the semen samples were all negative. The isolates were submitted to Pulsed-field gel electrophoresis methodology and their 16S rRNA genes were sequenced. Fifty percent of ureaplasma recovered from sheep allowed for PFGE typing. Eleven isolates showed eight profiles genetically close to the bovine ureaplasmas. The 16S rRNA gene sequencing showed differences or similarities of isolates from sheep and goats, and the reference strains of bovine and human ureaplasma. Four clinical isolates from sheep were grouped separately. The studied ureaplasma isolates showed to be a diverse group of mollicutes.

OBJETIVO: Avaliar os efeitos do alongamento e/ou exercício resistido e destreinamento na performance funcional de idosos. MÉTODOS: Quarenta e cinco sujeitos foram divididos em 4 grupos: controle (CG; n=13; 66±6 anos), alongamento (SG; n=10; 69±6 anos), resistido (RG; n=13; 69±5 anos), e resistido e alongamento (RSG; n=9; 66±5 anos). O CG não realizou exercícios. Os grupos SG, RG e RSG realizaram aquecimento seguido de exercícios para membros inferiores 2 vezes por semana. O SG realizou 4 repetições de alongamento. O exercício resistido foi realizado com carga de 65% de 10 repetições máximas (RM) durante 5 semanas, 70% nas 4 semanas seguintes e 75% nas últimas 3 semanas. O RSG realizou ambos os exercícios. Antes, após 6, 12 semanas, e após 6 semanas de destreinamento, a aptidão cardiorrespiratória foi avaliada usando o teste dos seis minutos de caminhada (6MWT), a força muscular dos membros inferiores pelo teste de sentar e levantar de uma cadeira (SUCSD) e pressão arterial sistêmica pelo esfigmomanômetro e estetoscópio. Os resultados foram comparados pela ANOVA (p<0,05). RESULTADOS: Seis semanas de treinamento aumentaram a distância percorrida (6MWT) do RG e diminuíram o tempo do SUCSD no SG. No entanto, RG aumentou a pressão arterial sistólica (SBP) após o destreinamento comparada com o SG. A pressão arterial diastólica (DBP) diminuiu após 6 semanas para o RSG e 12 semanas para o SG. CONCLUSÕES: Seis semanas de alongamento ou treinamento resistido melhorou o desempenho funcional dos idosos. Porém, a DBP diminuiu após 6 semanas, com a associação do exercício resistido com o alongamento. Já o destreinamento, aumentou a SBP em idosos que treinaram exercício resistido isolado.

OBJECTIVE: To evaluate the effects of stretching and/or resistive exercise, followed by detraining, on the functional status of older people. METHODS: Forty-five subjects were divided into four groups: control (CG; n=13; 66±6 years), stretching (SG; n=10; 69±6 years), resistive exercise (RG; n=13; 69±5 years), and resistive exercise and stretching (RSG; n=9; 66±5 years). The CG did not perform any exercise. The SG, RG, and RSG had warm-up sessions prior to performing lower-body exercises twice a week. The SG performed 4 repetitions of stretching. Resistive exercise was performed at a load of 65% of 10 repetitions maximum (RM) for five weeks, 70% for the next four weeks, and 75% for the last three weeks of the program. The RSG performed both exercises. Cardiorespiratory capacity was evaluated using the 6-minute walk test (6MWT) at baseline, at the six- and 12-week follow-ups, and after a six-week period of detraining. Lower limb muscle strength was assessed using the stand up from a chair and sit down test (SUCSD), and blood pressure was measured using a sphygmomanometer and a stethoscope. The results were analyzed using ANOVA (p<0.05). RESULTS: Six weeks of training increased walking distance (6MWT) in the RG and decreased SUCSD time in the SG. However, detraining increased systolic blood pressure (SBP) in the RG compared to the SG. Diastolic blood pressure (DBP) decreased after six weeks in the RSG and 12 weeks in the SG. CONCLUSIONS: Six weeks of stretching or resistive training can improve the functional status of older people. Nevertheless, DBP decreased after six weeks with the combination of resistive exercise and stretching. Detraining increased SBP when resistive exercise alone was used.

Pesquisou-se Mycoplasma spp, Ureaplasma spp e Acholeplasma laidlawiii em amostras de muco vaginal de 60 ovinos, criados na região de Piedade no Estado de São Paulo, Brasil, que apresentavam ou não vulvovaginite no exame específico do sistema genital. A caracterização desses microrganismos baseou-se no cultivo e detecção do respectivo DNA pela Reação da Polimerase em Cadeia (PCR) com os primers para classe Mollicutes (GPO e MGSO), para o gênero Ureaplasma (UGPF e UGPS) e a espécie Acholeplasma laidlawii (UNI e ACH3). A presença de micoplasmas não foi associada com distúrbios do trato reprodutivo dos animais, entretanto todos os isolados obtidos de Ureaplasma spp foram provenientes de animais com distúrbios reprodutivos, sugerindo o possível envolvimento desse agente nas enfermidades da reprodução. A PCR para a espécie Acholeplasma laidlawii detectou somente uma amostra positiva.

It was evaluated the presence of Mycoplasma spp, Ureaplasma spp and Acholeplasma laidlawiii in 60 samples of ovine vaginal mucous with the presence or absence of vulvovaginitis in the specific exam of the reproductive tract. The microorganisms were characterized based on bacteriological culture and DNA detection by Polymerase Chain Reaction (PCR) with specific primers to Mollicutes (GPO and MGSO), Ureaplasma (UGPF and UGPS) and Acholeplasma laidlawii (UNI and ACH3). The presence of mycoplasmas could not be associated with reproductive disorders in animals. The PCR to Acholeplasma laidlawii detected only one positive sample. However, all isolations of Ureaplasma spp were from animals presenting reproductive disorders, suggesting a possible involvement of this agent in reproductive diseases.

Foram examinadas 713 vacas de três rebanhos leiteiros localizados na região norte do Estado do Paraná e sudoeste do Estado de São Paulo, das quais 137 apresentaram mastite. Nas três propriedades foram detectados oito animais (1,12%) com mastite clínica por Mycoplasma bovis. Destes animais, quatro tratados com oxitetraciclina e tilosina e três com enrofloxacina, não responderam ao tratamento e foram descartados no decorrer da lactação. Uma vaca medicada com enrofloxacina recuperou quase que totalmente a secreção láctea mas a eliminação de M. bovis persistiu por toda lactação. Esta vaca apresentou cura bacteriológica na lactação seguinte. O descarte dos animais positivos, monitora-mento bacteriológico e a aplicação correta das medidas de prevenção para as mastites contagiosas controlaram a disseminação de M. bovis nos rebanhos.

In this study 713 cows were examined. The animals were from three dairy farms in northern Paraná and the southwest of the State of São Paulo. From these cows, 137 had mastitis. On the three farms, 8 cows (1.12%) with Mycoplasma bovis mastitis were detected. Four were treated with tylosin and oxytetracyclin and three with enrofloxacin. There was no response to the treatments, and these animals were culled during the lactation period. One cow treated with enrofloxacin almost totally recovered milk production, but elimination of M. bovis continued during the lactation, and there was no bacteriological cure. This cow had a normal milk production in the next lactation period, without elimination of M. bovis. Culling of positive animals, the bacteriological study and correct application of preventive practices for contagious mastitis controlled the dissemination of M. bovis to other animals.

Foram analisadas 664 amostras de leite de 83 vacas primíparas da raça Holandesa. As amostras foram colhidas no primeiro e no sétimo dia após o parto. Das 664 amostras analisadas, 488 (73,50%) foram bacteriologicamente negativas e 176 (26,50%), positivas para microrganismos aeróbios. Foi observado um alto índice de mastite clínica (20,48%). Os agentes isolados com maior freqüência foram os Staphylococcus spp coagulase negativo (64,20%), Staphylococcus spp coagulase positivo (8,52%), Streptococcus spp (7,96%), Actinomyces pyogenes (4,55%), Mycoplasma bovigenitalium (3,40%) e Escherichia coli (2,84%). Foi observado um maior índice de isolamento de patógenos no primeiro dia (17,62%) em relação ao sétimo (8,88%).

A total of 664 foremilk samples from 83 Holstein cows were cultured. Quarter samples were collected at parturition and 7 days post the first parturition. From 664 milk samples examined, 488 (73.50%) were bacteriologically negative and 176 (26.50%) were positive. A high incidence (20.48%) of clinical mastitis was observed. The most frequently encountered isolates were coagulase negative staphylococci (64.20%), coagulase positive staphylococci (8.52%), streptococci (7.96%), Actinomyces pyogenes (4.55%), Mycoplasma bovigenitalium (3.40%) and Escherichia coli (2.84%). The frequency of mastitis pathogen isolation was highest at parturition (17.62%) and decreased markedly during the first week (8.88%).