In Argentina, rabies is limited to some northern provinces. Availability of new vaccines abolishing the handling of the rabies virus and allowing disease control has regional and national strategic importance. Vaccines based on recombinant poxviruses have been successfully used as antirabic vaccines worldwide. Although these systems are not commercially available, the platform to obtain recombinant canarypox viruses (CNPV) has been previously set up in our laboratory. The aim of this work was the development and evaluation of an antirabic vaccine candidate based on recombinant CNPV expressing the rabies virus (RV) glycoprotein G (RG). A recombinant virus (CNPV-RG) expressing the RG coding sequence was designed. Inoculation of mice with this virus induced high RV seroneutralizing antibodies (3.58 and 9.76 lU/ml after 1 or 2 immunizations, respectively) and protected 78% of intracerebrally RV-challenged animals. In addition, it was determined that CNPV-RG has a relative potency of 3.5 lU/ml. The obtained results constituted the first stage of CNPV-RG evaluation as antirabic vaccine candidate. Further assays will be necessary to confirm its utility in species of veterinary Interest.

En la Argentina, la rabia está circunscripta a algunas provincias del norte. La disponibilidad de nuevas vacunas que eliminen la manipulación del virus rábico y que permitan el control de la enfermedad es de importancia estratégica nacional y regional. Las vacunas basadas en poxvirus recombinantes se han utilizado con éxito como vacunas antirrábicas a nivel mundial. SI bien estos sistemas no están disponibles comercialmente, la plataforma de obtención de virus canarypox (CNPV) recombinantes ya ha sido implementada en nuestro laboratorio. El objetivo de este trabajo fue obtener y evaluar un candidato a vacuna antirrábica basado en CNPV recombinantes que expresan la glicoproteína G (RG) del virus rábico (RV). Se construyó un virus recombinante que expresa la secuencia codificante de RG (CNPV-RG). La inoculación de ratones con este virus indujo altos títulos de anticuerpos seroneutralizantes de RV (3,58 y 9,76 Ul/ml después de una o dos inmunizaciones, respectivamente) y protegió al 78 % de los animales desafiados intracerebralmente con RV. Además, se determinó que el CNPV-RG posee una potencia relativa de 3,5 Ul/ml. Los resultados obtenidos constituyen la primera etapa en la evaluación del CNPV-RG como candidato a vacuna antirrábica. Se requerirán nuevos ensayos para confirmar su utilidad en especies de interés veterinario.

Este artigo revisa a trajetória intelectual de Rubén Darío, perfilando a configuração do artista no modernismo (1880-1910), primeiro movimento concreto entre os escritores hispano-americanos, que renova substancialmente a produção literária em língua espanhola. Incorporado ao mercado moderno como jornalista na condição de migrante, oriundo do contexto cultural arcaico da Nicarágua natal, até os mais destacados centros hispano-americanos (Buenos Aires) e europeus (Madri, Paris), são consideradas suas demandas de autonomia estética e sua constante afirmação da autoridade do poeta para intervir nas discussões sobre a sociedade e a cultura moderna. Aí se destaca sua aposta na criação de uma América Latina fundada em novos laços de solidariedade e união com a Espanha, diante do avanço ameaçador dos Estados Unidos.

This article reviews the intellectual career of Rubén Darío, profiling the artist's position in modernism (1880-1910), the first concrete movement among Hispano-American writers, which substantially renewed the literary output in Spanish. Incorporated into the modern market as a journalist and immigrant, the article follows Darío's intellectual life from the archaic cultural context of his native Nicaragua to some of the foremost Hispano-American (Buenos Aires) and European (Madrid, Paris) centres, and considers his demands for aesthetic autonomy and his constant affirmation of the poet's authority to intervene in discussions relating to modern society and culture. Here we see the emergence of his commitment to the creation of a Latin America founded on new ties of solidarity and union with Spain, in the face of the threatening advance of the United States.