ABSTRACT This study was carried out to evaluate the effect of a blend of additives (choline, methionine, selenium, and organic zinc) on performance, feed efficiency, rumen parameters, and carcass traits of Nellore heifers finished with roughage-free diet. Nellore heifers (n = 36; average BW = 244±24.1 kg; average 24 months of age) were maintained in a feedlot system for 86 d. Heifers were separated into two groups: control and additive. Heifers in control group were fed a based diet composed of 850 g kg−1 corn grain and 150 g kg−1 of a mineral-vitamin-protein pellet. The additive group was fed a diet supplemented with a blend of choline, methionine, selenium, and organic zinc at 1,667, 4,000, 1, and 24.37 mg kg−1 of the diet dry matter, respectively. The animals were allotted to 18 pens (two heifers/pen), with nine pens per treatment. Heifers were weighed, blood samples were collected, ultrasonography examinations were performed periodically, and hot carcass and papillae samples were taken at slaughter. Data were analyzed as a completely randomized design. The model included the fixed effect of treatments (control and additive). Additive supplementation did not change dry matter intake, performance, or feed efficiency. There was no effect of additives on muscle or fat tissue deposition. Consequently, no changes in hot carcass weight and dressing were found. Overall, additive inclusion did not alter blood parameters, blood electrolyte balance, and rumen traits. Nellore heifers finished with roughage-free diets have no improvement on production traits nor in their rumen health by supplementation with a blend of choline, methionine, selenium, and organic zinc.

ABSTRACT The objective of this study was to evaluate performance, hematological parameters, leukocyte counts, and respiratory microbiota of broilers under heat stress receiving eucalyptus oil supplementation. Cobb broilers (n = 1200) were randomly distributed on the first day of life in a factorial arrangement with additional treatment (2 × 2 + 1) with two periods of oil administration via drinking water (daily, from 18 to 35 days of life) and two periods of oil administration via spray (daily, from 18 to 35 days of age) plus a control treatment, consisting of twelve replications, with 20 animals each. The birds were kept in a controlled environment with constant temperature at 32 °C to induce heat stress by infrared heaters. Eucalyptus oil was supplied by drinking water (1 L of oil to 4000 L of water) or by spray applications with a pump (1 L of oil to 200 L of water). Bird weight was recorded at 42 days of age, along with remaining feed, to determine weight gain, feed intake, and feed conversion. In addition, one blood sample from each bird repetition was collected for hematological and leucocytic evaluation. To assess respiratory microbiota, a tracheal flushing was performed for bacteria counts. There was no difference in performance, hematological parameters, and leukocyte counts, except hematocrit, which was lower in birds that received eucalyptus oil after 18 days of age. No significant differences were observed in the respiratory microbiota comparing oil-treated and -untreated groups. Although it was not possible to verify statistical difference, the birds that received eucalyptus oil via spray plus spray from 18 days old showed lower bacterial counts and absence of isolation of Gram negatives, while the control group was the one with the highest number of Gram negatives. Therefore, eucalyptus oil can be used for heat-stressed broilers without impairing their development.

RESUMO: Os aditivos são amplamente utilizados em dietas com elevada proporção de concentrado a fim de prevenir distúrbios metabólicos em bovinos. Este estudo foi desenvolvido para avaliar o efeito das diferentes fontes de cálcio, com ou sem monensina sódica na dieta, no controle da acidose ruminal de bovinos Nelore, recebendo, de forma abrupta, uma dieta com elevada proporção (92,3%) de concentrado. Oito bovinos portadores de cânulas ruminais foram distribuídos em um delineamento quadrado latino (4x4) duplo contemporâneo. Os tratamentos foram a adição de diferentes fontes de cálcio, calcário calcítico (LI) ou produto a base de alga calcária (CA), com (WIMO) ou sem (WOMO) a presença de monensina, à dieta base. A alga calcária, Lithothamnium calcareum, é um produto natural, renovável e fonte de carbonato de cálcio, podendo ser uma alternativa no controle desses distúrbios. A inclusão de LI, CA e monensina foi de 7,1g kg-1, 7,4g kg-1 e 30mg kg-1 MS, respectivamente. Não houve efeito das fontes de cálcio e da monensina sobre o consumo alimentar e concentração total dos ácidos graxos de cadeia curta. Os tratamentos com AC resultaram em maior pH ruminal médio (P=0,039), menor tempo com pH ruminal abaixo de 5,2 (P<0,001) e um maior pH sanguíneo (P=0,006). Os tratamentos com monensina apresentaram menor tempo com pH ruminal abaixo de 5,2 (P=0,023). O produto a base de alga calcária foi eficiente no controle das mudanças adversas das variáveis ruminais e sanguíneas em bovinos Nelore submetidos a mudanças abruptas para dietas com elevada proporção de concentrado.

ABSTRACT: Additives are used in high concentrate diets to prevent metabolic disorders in cattle. This study was designed to evaluate the effect of calcium sources and monensin on the control of ruminal acidosis in Nellore cattle that were abruptly shifted to a high (92.3%) concentrate diet. Eight cannulated steers were randomly assigned to two contemporary 4x4 Latin square. Treatments involved the addition of a calcium source, either limestone (LI) or a product derived from calcareous algae (CA), to the basic diet with or without the presence of monensin. Calcareous alga (Lithothamnium calcareum) is a natural and renewable product and a source of calcium carbonate. The quantity of added limestone, calcareous algae and monensin was 7.1g kg-1, 7.4g kg-1 and 30mg kg-1 DM, respectively. There was no effect of calcium source (P=0.607) or monensin (P=0.294) on feed intake or on the concentration of short chain fatty acids. Treatments with calcareous algae resulted in a higher mean ruminal pH (P=0.039), a shorter amount of time with the ruminal pH under 5.2 (P<0.001) and a better control of blood pH (P=0.006). Treatments with monensin also resulted in a shorter amount of time with the ruminal pH below 5.2 (P=0.023). Calcareous algae were shown to be effective in controlling adverse changes in the rumen and in blood variables for Nellore cattle that were subjected to an abrupt change to a high concentrate diet.