Figure 1 - Immunolocalization of the glucose transporter GLUT2 in the plasma membrane of B-cells from cultured neonatal rat islets. The presence of GLUT2 was revealed in sections (10 µm) of rat islets by indirect immunofluorescence using a polyclonal rabbit GLUT2 antiserum (dilution 1:1000) and a fluorescein isothiocyanate (FITC)-conjugated specific second antibody (dilution 1:100). Fluorescence was detected by confocal laser scanning microscopy. Note the bright reaction at the cell membrane level following GLUT2 immunolocalization (arrow).