Figure 2 - Effect of glucose and prolactin (PRL) treatment on GLUT2 protein content measured by immunoblotting. Neonatal rat islets were cultured for 7 days in medium containing or not 2 µg/ml PRL and glucose at different concentrations. Neonatal islet protein (50 µg/lane) was analyzed by immunoblotting with antiserum to GLUT2. This is a representative blot of two.