Figure 1 - Ap4A (A) and Ap5A (B) hydrolysis as a function of synaptosome protein concentration. The assay mixture contained 20 mM Tris/HCl, pH 7.5, 2.0 mM MgCl2, 10 mM glucose and 225 mM sucrose in a final volume of 200 Ál. The samples were incubated for 60 min at 37oC. The data are representative of three independent experiments with different synaptosome preparations.