Figure 2 - Expression of epitopes defined by mAbs 1D9, 2C2, 4B5 and 4B9 is variable among amastigotes obtained from different sources. Amastigotes from clone D11 were isolated from infected cells (Intra), LIT medium after 48 h (Extra) or infected Vero cell supernatants (TCA), and studied by ELISA. Bars indicate mean value plus one standard deviation for a quintuplicate determination. mAb anti-p30 was used as a negative control.