Figure 3 - Expression of epitopes defined by mAbs 1D9, 2C2, 4B5 and 4B9 is variable among amastigotes of different T. cruzi strains and clones. Extracellular amastigotes were obtained in LIT medium after a 48-h incubation and analyzed by ELISA. Bars indicate the mean subtracted from the background values obtained with mAb anti-p30 (always lower than 0.2) plus one standard deviation for a quintuplicate determination.