Figure 1 - Construction of transfer vectors pAcOBtm and pAcOBt73. Plasmids pUCBtm and pUCBt73, containing the cry1Ab and cry1Ac genes, respectively, were digested with BamHI and the fragments containing the genes were cloned into plasmid pAcUW2B previously digested with BglII. The large arrows in pUCBtm, pUCBt73 and pAcUW2B represent the open reading frames of the cry1Ab, cry1Ac and polyhedrin (P) genes, respectively. The small arrow in pAcUW2B represents the p10 promoter.