Figure 2 - SSCP-PCR analysis of mixtures of normal and mutated exon 6 of the p53 gene. Different amounts of recombinant pUC18 bearing normal or mutated (9 nucleotide deletion) sequences were submitted to the reaction mix, in a final DNA concentration of 50 ng, either alone (lane 1: mutant; lane 8: normal) or mixed (lane 2: 25 ng each; lane 3: 20 ng mutant, 30 ng normal; lane 4: 15 ng mutant, 35 ng normal; lane 5: 10 ng mutant, 40 ng normal; lane 6: 5 ng mutant, 45 ng normal; lane 7: 2.5 ng mutant, 47.5 ng normal). Arrows indicate the banding pattern of single-stranded (SS) DNA and heteroduplex.