Figure 1 - Effect of MgCl2 (2.5, 5.0 or 7.5 mmol/kg, sc) either alone or in combination with diazepam (DZ) (MgCl2 + DZ, 5 mmol/kg and 10 mg/kg, ip, respectively) on hippocampal pyramidal cell loss after 15-min transient forebrain ischemia. The effect of 5.0 mmol/kg MgCl2 was tested after a single dose (1x, 2 h post-ischemia) or multiple (4x, 1, 2, 24 and 48 h post-ischemia) doses. The 2.5 and 7.5 mmol/kg MgCl2 doses were tested after multiple administration (4x) only. The animals treated with a single dose (1x) or multiple (4x) doses of MgCl2 (5.0 mmol/kg) were pooled, as were the groups treated with MgCl2 (1x) + DZ or MgCl2 (4x) + DZ. Diazepam (10 mg/kg), alone or in combination with MgCl2, was always given twice (1 and 2 h after reperfusion). Bars and vertical lines represent the mean and SEM. The numbers within brackets indicate the sample size. *P<0.0001 vs the sham-operated group.