Figure 2 - Size exclusion in HPLC of trypsinogen GdnHCl unfolding. The column (Shodex - Protein KW 803 (0.8 x 30 cm), Showa Denko & Shoko) was loaded with 50 µg of protein per run and equilibrated with 100 mM MES, pH 7.0, 20 mM CaCl2 and 81 mM NaNO3 buffer, pH 7.0, containing GdnHCl from 0 M to 3 M, respectively. Trypsinogen concentration was 1 mM in each sample. The flow rate was 1 ml/min and elution was monitored by absorbance at 280 nm. The profiles are shown with their respective retention times in minutes and the concentration of GdnHCl in molar concentration. The Stokes radii were calculated according to Uversky (21).