Figure 4 - Size exclusion in HPLC of trypsinogen urea denaturation. The column (Shodex - Protein KW 803 (0.8 x 30 cm), Showa Denko & Shoko) was loaded with 50 g of protein per run and equilibrated with 100 mM MES, pH 7.0, 20 mM CaCl2 and 81 mM NaNO3, pH 7.0, containing urea at different concentrations. Trypsinogen concentration was 1 mM in each sample. The flow rate was 0.75 ml/min and elution was monitored by absorbance at 280 nm. Profiles present the retention times in minutes for each urea concentration. The Stokes radii were calculated according to Uversky (21).