Figure 4 - Inhibitory effect of indomethacin on the increased response of the second phase (10-60 min) of the 5% formalin test in cannulated (Cn) animals. Panels A and B show the effects of direct injections of indomethacin (IND, 10 µg/10 µl) administered 30 min before subcutaneous injection of formalin to naive (Nv) and Cn animals. Panel C shows the effect of IND administration through a cannula. The controls (C) received 10 µl of Tris-buffer. The bars represent the mean ± SEM number of flinches of the injected paw of 9-12 animals per group. The asterisks indicate significant differences (*P<0.01 and **P<0.001, unpaired Student t-test).