Figure 3. The expression pattern of DDX26 in normal tissues and tumor cell lines. A 32P-labeled probe was hybridized under stringent conditions to a Human Master Blot (Clontech) containing normalized loadings of polyA+ RNA from 75 different human tissues (A) as well as to a Cancer Cell Lines Northern blot (Clontech) containing 2 µg of polyA+ RNA (C). B, Location of the various RNAs on the dot blot filter is shown schematically. C, For the Northern blot membrane, the cancer cell lines are: 1, melanoma, G-361; 2, lung carcinoma, A-549; 3, colorectal adenocarcinoma, SW480; 4, Burkitt's lymphoma, Raji; 5, lymphoblastic leukemia, MOLT-4; 6, chronic myelogenous leukemia, K-562; 7, HeLa S3, and 8, promyelocytic leukemia, HL-60. Molecular markers (kb) are indicated.