Figure 2 - Total glycosaminoglycans (A) and ³⁵S-labeled glycosaminoglycans (B) extracted from explants of intact or epithelium-debrided human corneas. Total glycosaminoglycans were stained with toluidine blue and quantified by densitometry. The ³⁵S-glycosaminoglycans were visualized by radioautography and scraped off the gels, and radioactivity was measured with a liquid scintillation spectrophotometer. Data are reported as means ± SD of 14 experiments. GAG, Total glycosaminoglycan; DS, dermatan sulfate; KS, keratan sulfate; HS, heparan sulfate.