Figure 1. SDS-PAGE of purified Tamm-Horsfall protein (THP). Proteins were loaded onto 10% polyacrylamide gels in the presence of a-mercaptoethanol and stained with silver nitrate. Lane A, Molecular mass standards of thyroglobulin (330 kDa), ferritin-half unit (220 kDa), bovine albumin (67 kDa), catalase (60 kDa), lactate dehydrogenase (36 kDa), and ferritin (18.5 kDa); lanes B-D, THP purified by gel filtration chromatography on a Sepharose CL-6B column; lane E, standard THP; lane F, molecular mass standards of phosphorylase B (94 kDa), bovine albumin (67 kDa), ovalbumin (43 kDa), carbonic anhydrase (30 kDa), trypsin (20.1 kDa), and a-lactalbumin (14.4 kDa).