Figure 1. Effects of dose and volume on in vivo myotoxicity of Bothrops jararacussu venom in mice. A, The venom protein dose was increased from 0.5 to 2.0 µg/g in the same volume (50 µl). Plasma creatine kinase (CK) levels were measured 2 h after venom injection. B, Effect of injection volume. Venom, 0.5 µg/g was injected in increasing volumes, 12.5 to 100 µl, and plasma CK levels were measured 2 h after injection. In both protocols the control group received 50 µl of 0.9% NaCl. Data are reported as means ± SEM (N = 4). *P<0.05 compared to the control group (ANOVA).