Figure 2. Immunoprecipitation with anti-Hsp47 and anti-Sec61a antibodies and collagenase digestion of retinoic acid-treated membrane-bound collagen IV microsomes following treatment with DSP. Microsomes were immunoprecipitated with anti-Hsp47 (lanes 1 and 3) and anti-Sec61a (lanes 2 and 4) antibodies. Collagenase digestion completely disrupted collagen microsomes (lanes 3 and 4).