Figure 3. Distribution of translocating chain-associating membrane protein (TRAM) and Sec61a using the Western blotting technique with enhanced chemiluminescence following retinoic acid (RA) treatment. F9 cells were incubated at 37ºC with (lanes 2 and 4) or without (lanes 1 and 3) retinoic acid for 4 days. Antibodies against TRAM (lanes 1 and 2) and Sec61a (lanes 3 and 4) were applied and the nitrocellulose paper was then exposed to radiographic film.