Figure 4. Distribution of Sec61a using the Western blotting technique with enhanced chemiluminescence following retinoic acid (RA) and heat shock (HS) treatment. F9 cells were either incubated at 37ºC (control cells, lanes 1 and 2) or at 43ºC (heat-shocked cells, lanes 3 and 4) for 90 min. In some cases, F9 cells were treated with all-trans retinoic acid for 4 days (lanes 2 and 4). Antibodies against Sec61a were added and the nitrocellulose paper was then exposed to radiographic film.