Figure 20

Fig. 20: Western-blot of S-antigen, purified from bovine retina, recognized by serum samples of Calomys callosus, immunized with: lane 1, S-Antigen (50 mg); lane 2, S-Antigen (50 mg) and CFA (1 mg/ml); lane 3, S-Antigen (50 mg) and jacalin (100 mg); lane 4, S-Antigen (50 mg), jacalin (100 mg) and CFA (1 mg/ml); lane 5, jacalin (100 mg); lane 6, CFA (1 mg/ml); lane 7, CFA (1 mg/ml) and jacalin (100 mg); lane 8, 20 cysts of Toxoplasma gondii, strain ME-49,by oral route, after 47 days of infection; lane 9, 20 cysts of T. gondii, strain ME-49, by oral route, after 21 days of infection and jacalin (100 mg); lane 10, positive serum sample control; lane 11, negative serum sample control.