Fig. 2: frequency of cytokine and iNOS producing cells in lesions from localized cutaneous leishmaniasis (LCL) and disseminated cutaneous leishmaniasis (DL) patients. Expression of IFN-g, TNF-a or iNOS was quantified by immunohistochemistry using anti-IFN-g, anti-TNF-a or anti-iNOS antibodies. Negative no positive cells; low up to 20% of positive cells; moderate 21% to 50% of positive cells; or high if more than 50% of positive cells. Mean is indicated by bars and standard deviation by lines above bars. Significance levels are indicated.