Decorin is one of the proteoglycans expressed in Walker 256 rat mammary carcinoma. S.M. Oba-Shinjo, A.G.A. Berto, C.C. Passerotti, C.D. Barbosa and L.O. Sampaio. Brazilian Journal of Medical and Biological Research, 36 (8): 1079, 2003.
Figure 3. Sepharose CL-4B elution profiles of proteoglycans from Walker 256 carcinoma. Fractions Oc-1M and Oc-6M from Octyl-Sepharose CL-4B were chromatographed on Sepharose CL-4B, which was equilibrated and eluted with 4 M GuaHCl, 50 mM sodium acetate, pH 5.8. Protein (20 µl) and sulfated glycosaminoglycans (30 µl) were determined by absorbance and radioactivity, respectively, in the 2 ml fractions collected. Fractions containing proteoglycans were pooled as indicated by the upper horizontal bar in the lower panel, dialyzed and lyophilized for further purification. V0 = void volume; Vt = totally included volume.