Sulfated proteoglycans as modulators of neuronal migration and axonal decussation in the developing midbrain. L.A. Cavalcante, J. Garcia-Abreu, F.A. Mendes, V. Moura Neto, L.C.F. Silva, G. Onofre, G. Weissmüller and S.L. Carvalho. Brazilian Journal of Medical and Biological Research, 36 (8): 993, 2003.

Figure 3. Photomicrographs showing predominant morphologies of MAP2-stained E-14 midbrain neurons after culturing for 24 h onto lateral (A, B) and medial (C, D) astrocytes. A and C, Neurons grown onto a substrate of lateral (A) or medial astrocytes (C) without enzymatic treatment; B and D, Neurons in co-cultures treated with heparitinase 1 (hept). Notice that hept treatment reverses the inhibitory properties of medial astrocytes (D) as also shown by ß3 tubulin staining (cf. Figure 5 in Ref. 40). Scale bar: 50 µm. L = lateral; M = medial. Reproduced from Ref. 36, courtesy of Academia Brasileira de Ciências.