Use of blends of bioabsorbable poly(L-lactic acid)/poly(hydroxybutyrate-co hydroxyvalerate) as surfaces for Vero cell culture. A.R. Santos Jr., B.M.P. Ferreira, E.A.R. Duek, H. Dolder and M.L.F. Wada. Brazilian Journal of Medical and Biological Research, 38 (11): 1623, 2005.
Figure 3. Scanning electron microscopy of Vero cells cultured on the polymers for 24 h. A, Cells cultured on a glass coverslip. The cells are either elongated or flattened. B, PLLA/PHBV (100/0) membrane with round cells growing in a pore, with microvilli and thin filaments. C, A non-porous region of the same polymer. In this area, the cells are flattened. D, Cells on a (60/40) PLLA/PHBV blend. Cells had a growth pattern similar to that of the previous samples, although in the porous regions they were more irregular in structure. E, Cells on a (50/50) PLLA/PHBV blend. Flattened cells with filaments grow in similar proportions on porous or non-porous areas. F, Cells on a (40/60) PLLA/PHBV blend. Flattened cells showed shrinkage at their edges and grew preferably on non-porous areas. G) Cells on a (0/100) PLLA/PHBV membrane. In this case, we found thin filaments linking the cells. H, Sample showing a flattened cell on a (0/100) PLLA/PHBV membrane at high magnification. In the figures, the porous area is represented by asterisk. Scale bar: 20 µm for figures A and G, 5 µm for B, C and H, and 10 µm for other figures. PLLA = poly(L-lactic acid); PHBV = poly(hydroxybutyrate-co-hydroxyvalerate).