Lima-Leopoldo, A.P.; Sugizaki, M.M.; Leopoldo, A.S.; Carvalho, R.F.; Nogueira, C.R.; Nascimento, A.F.; Martinez, P.F.; Luvizotto, R.A.M.; Padovani, C.R.; Cicogna, A.C. Obesity induces upregulation of genes involved in myocardial Ca2+ handling. Braz J Med Biol Res 2008; 41: 615-620.
Figure 2. Effect of high-fat diet-induced obesity on the expression of genes related to Ca2+ transport in the rat. RT-PCR was carried out for L-type Ca2+ channel (Cacna1c; 604 bp, 28 cycles), Na+/Ca2+ exchanger (NCX; 634 bp, 28 cycles), Ca2+ ATPase (SERCA2a; 653 bp, 28 cycles), ryanodine receptor (RyR2; 635 bp, 28 cycles), and phospholamban (PLB; 140 bp, 30 cycles). Gene expression was normalized to cyclophilin expression (440 bp). Experiments on control (C, N = 13) and obese (Ob, N = 13) rats were performed in triplicate and PCR quantification was by densitometry analysis. *P < 0.05 vs control (Student t-test for independent samples).