. Modulation of extracellular matrix by nutritional hepatotrophic factors in thioacetamide-induced liver cirrhosis in the rat.
Figure 4. Photomicrographs of a cirrhotic liver from an untreated rat submitted to different techniques. a, High proliferation activity of bile duct epithelial cells is seen in the area of bile duct proliferation (arrows). b, Bile duct epithelial cells are strongly positive to progranulin (Pgrn) protein in a proliferation area (arrowhead). c, Progranulin in situ hybridization (ISH) in the area of bile duct proliferation. The bile duct epithelial cells are strongly positive. d, Non-parenchymal cells also show high proliferation activity in a fibrous septum area (arrows). e, Non-parenchymal cells show progranulin protein positivity (asterisk). f, Progranulin ISH in a fibrous septum area, showing high positivity of non-parenchymal cells (asterisk). Panels a and d are diaminobenzidine immunostaining and hematoxylin counterstaining. Panels b and e are diaminobenzidine immunostaining. BrdU = bromodeoxyuridine; IHC = immunohistochemistry. Bar: 100 μm.