M.C.S. Brum, R. Weiblen, E.F. Flores and S.I. Chowdhury. Construction and growth properties of bovine herpesvirus type 5 recombinants defective in the glycoprotein E or thymidine kinase gene or both.
Figure 3. PCR amplification of the BoHV-5 TK gene internal region (285 bp). Madin Darby bovine kidney cells were mock infected or inoculated with wild-type (wt) or recombinant viruses and total DNA was extracted and submitted to PCR amplification of the BoHV-5 TK gene. A positive reaction indicates the presence of the entire TK gene. Five microliters of each reaction was resolved on 1.5% agarose gel and stained with ethidium bromide. Lane 1, Molecular weight marker; lane 2, MDBK mock; lane 3, BoHV-5 wt; lane 4, BoHV-5 TK∆, and lane 5, BoHV-5 gE/TK∆.