T. Fernandes, N.Y. Hashimoto and E.M. Oliveira. Characterization of angiotensin-converting enzymes 1 and 2 in the soleus and plantaris muscles of rats. Braz J Med Biol Res 2010; 43: 837-842.

Figure 1. ACE1 in the skeletal muscle. A, Skeletal muscle ACE1 activity reported as means ± SEM for 8 rats in each group. B, Representative blots of plantaris ACE1, soleus ACE1, and α-tubulin. C, Representative figure of soleus and plantaris ACE1 protein expression in Wistar rats. Note that the plantaris muscle displayed higher activity than the soleus and that both muscles presented a dose-response curve for ACE1 protein expression due to an increase in protein (30, 50, 80, and 100 µg protein). Also, the plantaris muscle presented increased ACE1 protein expression compared to the soleus muscle. The rat lung was used as positive control (Ct+) for ACE1 activity and protein expression. ACE = angiotensin-converting enzyme. *P < 0.05 compared to soleus muscle; #P < 0.0001 compared to soleus and plantaris muscles; P < 0.01 compared to soleus muscle (Student t-test).