The effect of microgravity on tissue structure and function of rat testis. Ye Ding, Jin Tang, Jun Zou, Ruiping She, Yinghua Wang, Zhuo Yue, Jijing Tian, Kangkang Xia, Jun Yin and Desheng Wang. Braz J Med Biol Res 2011; 44: 1243-1250.

Figure 3. Localization and quantitative analysis of androgen receptor (AR) with immunohistochemical staining. AR in the control (CON) groups (A, not treated for 7 days; C, not treated for 14 days) and tail-suspended (TS) groups (B, TS group for 7 days; D, TS group for 14 days). AR was detected using rabbit anti-AR antisera, biotinylated second antibody and horseradish peroxidase-labeled avidin chain working fluid. The immunohistochemical staining of AR resulted in brown or yellow color of the areas containing AR, which can be used to trace and measure AR. E, Relative signal intensity of AR in rat testis. *P < 0.01 compared to control (one-way ANOVA and Tukey test).