The effect of microgravity on tissue structure and function of rat testis. Ye Ding, Jin Tang, Jun Zou, Ruiping She, Yinghua Wang, Zhuo Yue, Jijing Tian, Kangkang Xia, Jun Yin and Desheng Wang. Braz J Med Biol Res 2011; 44: 1243-1250.

Figure 4. Localization and quantification of HSP70 by immunostaining. HSP70 in the control (CON) groups (A, control group for 7 days; C, control group for 14 days) and tail-suspended (TS) groups (B, TS group for 7 days; D, TS group for 14 days) was detected using rabbit anti-HSP70 antiserum, biotinylated second antibody and horseradish peroxidase-labeled avidin chain working fluid. E, The area density of positive substance was obtained by dividing the area of positive signals by the area of one field under the microscope with combined magnification of 400X. *P < 0.01 compared to control (one-way ANOVA and Tukey test).