In vivo and in vitro anti-inflammatory and anti-nociceptive activities of lovastatin in rodents. D.O. Gonçalves, I.B.F. Calou, R.P. Siqueira, A.A. Lopes, L.K.A. Leal, G.A.C. Brito, A.R. Tomé and G.S.B. Viana. Braz J Med Biol Res 2011; 44: 173-181.

Figure 4. Effects of lovastatin (LOV) on the release of human neutrophil myeloperoxidase (MPO) stimulated with phorbol myristate acetate (PMA). The cells were pre-incubated with LOV (0.01, 0.1 and 1 μg/mL) or indomethacin (INDO, 35.7 μg/mL) as the reference drug, prior to the addition of PMA. The suspension was centrifuged (10 min at 2000 g, 4°C). Supernatant aliquots (50 μL) were added to PBS (100 µL), phosphate buffer (50 µL, pH 7.0) and H2O2 (0.012%). After 5 min at 37°C, the substrate 3,3’,5,5’-tetramethylbenzidine (TMB, 1.5 mM, 20 µL) was added, the reaction stopped with 1.5 M sodium acetate, pH 3.0, and absorbance determined at 620 nm. Data are reported as percent inhibition of MPO release (mean ± SEM).