Essential regulatory elements for NHE3 gene transcription in renal proximal tubule cells. E.A. Neri, C.N.A. Bezerra and N.A. Rebouças.
Figure 3. Promoter activity of various 5’-deletion constructs of the proximal promoter region of the rat NHE3 gene. Firefly luciferase reporter constructs containing various lengths of the proximal NHE3 promoter were generated as described. The chimeric promoter-Firefly luciferase gene with different 5’-ends and a common 3’-end (+31) was cotransfected with pRL into opossum kidney, clone P (OKP) cells. After 24 h, the cells were lysed and assayed for Firefly and Renilla luciferase activities. The promoter activity is expressed relative to the activity of the promoterless vector and is normalized for variations in transfection efficiency using Renilla luciferase activity. Data are reported as means ± SEM for at least three independent transfection experiments performed in triplicate. The spanning region is indicated on the left side of each bar. Statistical analysis was carried out by ANOVA followed by the Tukey test.