Rhein induces apoptosis of human gastric cancer SGC-7901 cells via an intrinsic mitochondrial pathway. Yiwen Li, Yuqing Xu, Bo Lei, Wenxiu Wang, Xin Ge and Jingrui Li. Braz J Med Biol Res 2012; 45: 1052-1059

Figure 3. Rhein-induced apoptosis in SGC-7901 cells was determined by flow cytometry using the annexin FITC-PI staining method. The cells were treated with rhein (0, 100, 200, or 300 µM) for 48 h. The lower right quadrant indicates the percentage of early apoptotic cells (FITC-stained cells) and the upper right quadrant indicates the percentage of late apoptotic cells (FITC-PI-stained cells) (A). The experiment was repeated three times and the percentage of apoptotic cells (means ± SEM) for each treatment group is shown in B. *P < 0.05 versus control group (0 µM) (one-way ANOVA followed by the Tukey post hoc test).