Rhein induces apoptosis of human gastric cancer SGC-7901 cells via an intrinsic mitochondrial pathway. Yiwen Li, Yuqing Xu, Bo Lei, Wenxiu Wang, Xin Ge and Jingrui Li. Braz J Med Biol Res 2012; 45: 1052-1059

Figure 4. Rhein increased gene expression of caspase-9 and -3 in SGC-7901 cells in a dose-dependent manner. SGC-7901 cells were treated with rhein (0, 100, 200, or 300 µM) for 48 h. The expression of mRNAs was analyzed by real-time quantitative PCR and normalized by GAPDH expression. *P < 0.05 versus control group (0 µM) (two-way ANOVA followed by the Tukey post hoc test).