Rhein induces apoptosis of human gastric cancer SGC-7901 cells via an intrinsic mitochondrial pathway. Yiwen Li, Yuqing Xu, Bo Lei, Wenxiu Wang, Xin Ge and Jingrui Li. Braz J Med Biol Res 2012; 45: 1052-1059

Figure 5. Rhein decreased the expression of Bcl-2, Bcl-xL and pro-caspase-3 but increased the expression of the pro-apoptotic protein Bax in SGC-7901 cells. SGC-7901 cells were treated with rhein (0, 100, 200, or 300 µM) for 48 h and the expression of proteins in treated cells was determined by Western blot analysis. A, Rhein-induced changes in Bcl-2 and Bax expression and Bax:Bcl-2 ratio. B, Rhein decreased Bcl-xL. C, Rhein
decreased pro-caspase-3. Data are reported as the means ± SEM of at least three experiments. *P < 0.05 versus control group (0 µM) (two-way ANOVA followed by the Tukey post hoc test).