Cultivation and identification of colon cancer stem cell-derived spheres from the Colo205 cell line Ying-fei Li, Bing Xiao, San-fang Tu, Yuan-yuan Wang and Xiao-lang Zhang.
Figure 3. Flow cytometry dot plots show that cancer sphere cells expressed high levels of CD133 and CD44 in serum-free medium. Left, Isotype control. Right, Cancer sphere cells incubated with FITC-conjugated anti-CD44 and PE-conjugated anti-CD133 monoclonal antibodies. FITC = fluorescein isothyocyanate; PE = phycoerythrin.